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Adipogen/UBE2W Isoform 2 (human) (rec.) (His)/AG-40T-0448-C100/100 µg

  
  2024-05-07
  
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Ubiquitin-conjugating Enzyme E2 W; Ubc16; N-terminus-conjugating E2; Ubiquitin-protein Ligase W
Protein
E. coli
Human UBE2W Isoform 2 (Accession Nr. Q96B02-2) fused to a N-terminal His-tag.
Human
Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.
Use: Member of the ubiquitin­conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin­activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1­-1μM.
Manufactured by Boston Biochem
DRY ICE
-20°C
-80°C
Aliquot to avoid freeze/thaw cycles.
Stable for at least 1 year after receipt when stored at -80°C.
No
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Ubiquitin-conjugating Enzyme E2W (UBE2W), also known as ubiquitin-conjugating enzyme 16 (UBC16), is a member of the ubiquitin-conjugating (E2) enzyme family. It has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with ubiquitin. There are two isoforms of UBE2W, isoform 1 and isoform 2, with predicted molecular weights of 18.1 kDa and 19.5 kDa, respectively. UBE2W localizes to the nucleus where it promotes the mono-ubiquitination of BRCA1 and FANCD2, both of which contribute to DNA repair pathways. Mono-ubiquitination of the CHIP ubiquitin ligase (E3), which has been reported to enhance its E3 ligase activity, is also mediated by UBE2W.

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