| Product Name | Congo Red *UltraPure Grade* |
| Size | 1 g |
| Catalog # | AS-83016 |
| US$ | $68 |
Early diagnosis and classification of amyloid deposition and differentiation from other glomerular fibrillar deposits rely on routine Congo red (CR) histochemistry. CR binding, monitored by characteristic yellow-green birefringence under crossed polarization has been used as a diagnostic test for the presence of amyloid in tissue sections for several decades. This assay is also widely used for the characterization of in vitro amyloid fibrils. CR is sandwiched between two protein molecules causing protein oligomerization. Congo red fluorescence (CRF) is an alternative method based on examination of the CR-stained section by ultraviolet (UV) light. Congo red fluorescence is simple to perform and more pronounced, therefore easier to evaluate than CR in bright light. Congo red, when combined with immunohistochemistry, is still visible under UV whereas CR is masked in bright light. Although not widely used, the CRF method for detecting amyloid is simple to use with a high specificity and sensitivity, and may be applied successfully to frozen sections.
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| Detailed Information |  Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| References | 1. Tzankov A, et al. (2003). Acta Med Austriaca 30, 29-32.2. Roterman I, et al. (2001).Med Sci Monit 7, 771-84.3. Khurana R, et al. (2001).J Biol Chem 276, 22715-21. |
| Molecular Weight | 696.665 |
| Molecular Formula | C32H22N6Na2O6S2 |
| Spectral Properties | Abs/Em = 497/NA nm |
| Solvent System | water |
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我刚开始做转染，悬浮细胞，分别做过表达和敲减，看了很多文献，大都没有提及转染后是用转染的这同一批细胞同时做pcr，wb，cck8，凋亡，细胞周期；还是说这次转染只做pcr或wb，再转染一次做cck8或细胞周期。剩下的功能试验均同前，转染一次做一次？我养的是悬浮细胞，转染后做cck8这些功能试验前需要离心换液吗？跪谢解答！
我转的是7901、7901/DDP两种细胞，前者7901细胞很容易就转上，并且转后，状态良好，可是7901/DDP一转就死，我用的是吉玛慢病毒，转24小时后换液，刚开始一两天，没有异常，但后来细胞慢慢就死了，并且不是漂浮的，很多是贴着壁死，像是瓦解了一样
这是未转时细胞的样子
这是细胞转后，死亡的样子
并且即使是有些细胞未死，细胞后来也变得很脏，感觉有很破碎的细胞碎片
本人实验小白，**园子里大神指点，急，实在不知道怎么回事
前辈们好，近期老板布置任务需要分离小鼠肝脏细胞，查了好多文献，都没有写胶原酶的cat#,只写sigma订购，想请教下，是否有人做过，可以提供下货号和使用方法吗？十分感谢。另外，文中还提到digitonin，如果有一起用到的话麻烦帮我查看下，谢谢。
减数分裂也是真核生物细胞增殖的方式之一。
真核生物细胞增殖的方式有：有丝分裂、无丝分裂和减数分裂。
想用二步酶消法来分离毛乳头细胞，一个毛囊大概有多少个毛乳头细胞？处于生长期的毛囊在显微镜下有什么特点？先谢谢大神们指点
我最近跑电泳NF-kB信号通路，跑了p65和IkB-α两个蛋白，p65显影的时候有结果，但是IkB-α显示的时候是没有条带，空白一片，我跑电泳的条件不变、蛋白样品也是相同的，但是一个有结果，一个没有结果，请问原因是什么，请大神帮忙解答一下，谢谢啦~~
