AmplideX® PCR/CE SMN1 Kit
The AmplideX PCR/CE SMN1 Kit* provides a rapid, robust, and reliable method for the quantification of SMN1 exon 7 copy number from whole blood and buccal samples. With its streamlined PCR/CE workflow, rapid turnaround time, and automated results reporting software, the assay provides a simple and scalable SMN1 solution for all laboratories.
Features & Benefits
AmplideX technology has become widely established for the detection and quantification of repeat expansions in a number of genes, including FMR1, C9orf72, and DMPK. With the AmplideX PCR/CE SMN1 Kit, this approach is applied to copy number resolution, allowing SMN1 exon 7 copy number to be estimated in under four hours using readily available laboratory equipment.
Reduced Complexity
- Similar workflow to the AmplideX FMR1 kit*† eases implementation and testing
- Scalable design allows for high-throughput testing
- Automated analysis objectively reports copy number
Optimized Workflow
- A simplified workflow compared to alternative methods
- Fully kitted solutions with fewer QC and pipetting steps
- DNA to data in under four hours with only 45 minutes of hands-on-time
Quality Performance
- Ability to differentiate between 0, 1, 2, 3 and > 3 copies
- Robust, reliable results with non-overlapping ratios for each copy number output
- Concordance with previously characterized samples
*For Research Use Only. Not for use in Diagnostic procedures.†CE-IVD for US Export Only.
Analytical Characteristics
Analytical characteristics of AmplideX PCR/CE SMN1 Kit:
- Excellent concordance with previously characterized clinical samples (Figure 1)
- Consistent results compared to other commercially available methods (Figure 2)
- Automated results reporting via AmplideX Reporter software (Figure 3)
Figure 1. Excellent concordance with previously characterized clinical samples.
Figure 2: Consistent results compared to other commercially available methods.
Figure 3: Automated results reporting via AmplideX Reporter software.
Figure 4: Workflow for the AmplideX PCR/CE SMN1 Kit
Ordering Information
| Product Name | Number of Reactions | Catalog Number |
|---|---|---|
| AmplideX® PCR/CE SMN1 Kit (RUO) | 96 | 49660 |
| AmplideX® PCR/CE SMN1/2 Kit (RUO) | 50 | A00001 |
T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com
AmplideX® PCR/CE SMN1 Kit
The AmplideX PCR/CE SMN1 Kit* provides a rapid, robust, and reliable method for the quantification of SMN1 exon 7 copy number from whole blood and buccal samples. With its streamlined PCR/CE workflow, rapid turnaround time, and automated results reporting software, the assay provides a simple and scalable SMN1 solution for all laboratories.
Analytical Characteristics
Ordering Information
| Product Name | Number of Reactions | Catalog Number |
|---|---|---|
| AmplideX® PCR/CE SMN1 Kit (RUO) | 96 | 49660 |
| AmplideX® PCR/CE SMN1/2 Kit (RUO) | 50 | A00001 |
T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com
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能不能用kappa检验，我看了论坛上的帖子，好像是不行的。但是看到一篇文章，计数资料却用了kappa检验，请问能否这样使用，如果能的话，应该用什么软件，怎么计算。谢谢
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Na、K离子的通透性直接受膜电位的调节，如果指定某一电位(膜片钳钳制为某一电位)时，跨膜离子通透性一定，当然此时是可以通过测定电流来算出电导性， 其应该是某一不变的值；而膜片钳测定电导性的变化是怎么实现的？
我的上述思维里，肯定是有某个地方出现错误，苦于一直想不明白，求指点，谢谢！
平常都是用超声破清洗器水浴超声破碎大约20分钟，然后上样的
但是一方面，常温超声不知道会不会造成蛋白降解破坏，因为发现加冰以后超声会变得非常弱，所以都么加冰。另一方面，因为水浴超声声强是不均匀的，如果样品多的话，会发现有些已经超碎DNA，但有些还是一团。
不知道是否有其他更好的方法？
需要做脂肪代谢实验，打算通过在心肌新鲜组织匀浆中加入核素标记的脂肪酸代谢底物（[14C]-Palmitate），通过液体闪烁计数仪测定氧化产物[14C]CO2的放射性计数来反应脂肪酸的氧化代谢率。但是实验室只有γ放射免疫计数仪，请问能否用其测量“软β射线”14C；仪器需要作何调整吗？？？
