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Description
Details
Description: Mouse monoclonal antibody to human Tumor Necrosis Factor alpha (TNF-α)
Purification: Protein G affinity purified
Product Type:Primary antibody
Target Protein: Human TNF-α
Immunogen: Purified recombinant human TNF-α
Fusion Myeloma: Sp2/0-Ag14
Specificity:This antibody recognizes recombinant human TNF-a. Reactivity with natural TNF-a has not been tested.
Species Reactivity: Human, other species not tested
Host / Isotype: Mouse, IgG1 Kappa
Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Storage: Store at -20oC
Research Area: Cytokine, inflammation
Background:
Tumor Necrosis Factor-alpha (TNF-α) is a multifunctional pro-inflammatory cytokine, mainly secreted by activated macrophages. It is implicated with a variety of biological procedures including systemic inflammation, cell proliferation, apoptosis, lipid metabolism, and coagulation. The pleiotropic attribute of TNF-α regulation is associated with its ability to trigger multiple signalling pathways through its receptors, TNFR1 (p55) and TNFR2 (p75).
TNF-α not only contributes to the immune response to bacterial, fungal, viral and parasitic invasions, but also functions in tissue remodeling, autoimmune-diseases and the necrosis of specific tumors. TNF-α hyper-expression in response to some bacterial components such as LPS can cause life threatening septic shock. Recombinant TNF-α, in combination with chemotherapy, has been applied for treatment of soft sarcomas, melanomas and other irresectable tumors. Anti-TNF-α therapy has been used for treatment of rheumatoid arthritis.
Applications:
ELISA:React with recombinant human TNF-α.
Western Blotting
Figure:Western blot analysis of recombinant Human TNF-a using anti-human TNF-a mAb C4C7.
References: If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Human TNF-α, C4C7 |
|---|---|
| Application | EIA, WB |
| Size | 0.1 mg |
ebiomall.com
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看到之前的一个朋友也发了IBC会议的PPT内容，不过好像没有这个PPT（不确定，要是重复了请见谅）。
因为会议上投影的问题，当时有些内容贾老师没介绍太多。不过内容相当不错，主要是国内比较缺乏这方面的信息和经验。可以算得上是干货吧，不过里面主要是一些规则性或方向性的内容，需要具体细节，只能花钱请她做顾问了。
AudreyJia是美籍华人，在FDA做CMCReviewer之前也一直是从事细胞开发及细胞培养的工作。她应该也是目前唯一一个生物大分子部门中，唯一从FDA离职出来从事顾问工作的。可能很多国内单抗药企大佬都认识她，可能很多园子里面的人也见过她了。
谁能赏几个积分啊？新人，没积分，一些东西想看或者下载都不行。
版主jackieustc留言:
感谢分享，鼓励新人，我给你打赏，多交流自然有收获！
Monoclonality_JIA1.pdf(952.57k)
单纯从你这个尿常规结果来看：你的上皮细胞数量在正常女性的尿液中算是正常的，镜检白细胞：15——20个/HP，它表示你的泌尿系统可能存在感染。但是，因为没有你的其他资料和其他检查结果，单凭一个结果不能说明太多问题。一般情况下不可能是肾的不好，请不用担心。
我的建议是：
1、去医院复查，但要注意留尿的时候先清洁外阴，留取中段尿。及时化验。
2、如果检查结果仍然有白细胞，可去妇科或泌尿科看医生，一般的感染的话吃点药马上就好了。
3、放松心情，注意个人卫生即可。
指导意见：
毕竟阴道上皮是鳞状上皮,而输尿管,膀胱,尿道的上皮是柱状上皮,形态上是不一样的.所以您要是对您的结果出怀疑态度,首先：重新留尿,做一个复检（留尿时注意防止阴道分泌物污染）；或者让值班医生分析一下是那种类型的上皮（鳞状还是柱状）.所以不要担心这个结果.一般情况下污染的比较多.
