Hito Verhoeff OptimStain™ Kit is designed based on the Van Gieson and Verhoeff staining method with improved and simplified procedures. This kit can be used for simultaneously demonstrating the morphological details of collagen and elastic fibers.One example application of this kit is to determine the extent of atherosclerosis. The atherosclerotic plaque contains lipids, inflammatory cells, smooth muscle cells, connective tissue (eg, collagen, glycosaminoglycans, elastic fibers), thrombi, and calcium deposits. Fibrillar collagen is a critical component of atherosclerotic lesions. Uncontrolled collagen accumulation leads to arterial stenosis, while excessive collagen breakdown combined with inadequate synthesis weakens plaques thereby making them prone to rupture. With its special design, Hito Verhoeff OptimStain™ Kit will allow localization and visualization of the collagen and damage of elastic structure, therefore, enables a good understanding of the extent of atherosclerosis. This kit can also be used to demonstrate changes in elastic tissues in case of emphysema and other vascular diseases.Hito Verhoeff OptimStain™ Kit is made in a ready-to-use format and provides high quality, reliable and sensitive staining of collagen and elastic fibers.Hito Verhoeff OptimStain™ Kit has been tested extensively on the hearts and arteries from several species of animals and it is a simple solution for your research.
| Kit Contents (for >150 slides) | |
| Solution-1A | 125 ml |
| Solution-1B | 55 ml |
| Solution-1C | 55 ml |
| Solution-2 | 225 ml |
| Solution-3 | 225 ml |
| Solution-4 | 225 ml |
| Solution-5 | 225 ml |
| Staining jars | 5 |
| User Manual and MSDS | 1 |
Before using Hito Verhoeff OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Cryostat or Microtome, Light microscopeParaffin embedding equipment (or paraffin sections)Hito Bouin’s Plus Solution (Cat# HTSH0102, for paraffin sections preparation)Dry ice, isopentane, O.C.T. compound (for frozen sections), 4% PFA (Cat# HTSH0101), ethanol, xylene, double distilled or deionized waterSlides, coverslipsStaining jars for slides washResinous mounting medium  Hito Verhoeff OptimStain™ Kit Manual and MSDS | |
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希望有大神赐教!谢谢！
红细胞压积，是指红细胞占血液体积的比例，您仅仅是比参考范围略低，同时红细胞数量，和血红蛋白并没有下降，这可能是血液有所稀释所致。
白细胞计数3.9，正常的话 4-10之间，你仅仅是比参考范围略下降一丁点，本身并无意义，参考范围是大致的。而且血液还可能存在稀释可能性
中性粒细胞绝对值和百分比略低一点，同时淋巴细胞百分比略有上升，那这本身就只是略高一点点，而一般比如感冒以后，也往往可以升高，其他的红细胞分布宽度等是派生指标，没大意义的。
总之单单看这一个血常规检验，本身没有什么大的异常发现，当然也可以稍后复查，比如过2周以后复查
我是厉清，更多医疗知识，请看 厉清就是我 的新浪博客
G418浓度之前师兄已经摸索出来，这次筛选直接用师兄浓度。
1、6孔板转染后多长时间开始进行G418筛选？
2、G418筛选是在6孔板中继续筛选还是转到24/96孔？大皿？
3、G418浓度什么时候减量
4、？筛选过程中荧光观察一小部分克隆不错，如何挑选出来？
5、整个筛选过程大约需要多长时间？
lipo3000筛选
谢谢大家！
病情描述(发病时间、主要症状等)：
体检血常规中血小板计数343.血小板比积0.39.
尿检中白细胞计数26.2.上皮细胞计数10.30
心电图下写窦性心律，p-r间期偏短
想得到怎样的帮助：
有没有什么大问题
