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Addgene/pAAV-CAG-GFP/1unit/37825-AAV1.T

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Addgene

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37825-AAV1.T

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Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	37825	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart
AAV1	37825-AAV1	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV1 trial size	37825-AAV1.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV2	37825-AAV2	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV2 trial size	37825-AAV2.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV5	37825-AAV5	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV5 trial size	37825-AAV5.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV8	37825-AAV8	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV8 trial size	37825-AAV8.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV9	37825-AAV9	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV9 trial size	37825-AAV9.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV Retrograde	37825-AAVrg	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV Retrograde trial size	37825-AAVrg.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV PHP.eB	37825-PHPeB	Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information		$380	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
AAV with CAG promoter
(Search Vector Database)
Backbone manufacturer
Scott Sternson
Backbone sizew/o insert(bp)4722
Vector type
Mammalian Expression, AAV ; Adeno-associated virus

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
30°C
Growth Strain(s)
NEB Stable
Growth instructions
Use recombinase-free E. coli (Stbl3, XL-10, Sure2 and DH5a et.al), grow at 30C
Copy number
Low Copy

Gene/Insert

Gene/Insert name
GFP
Alt name
Green fluorescent protein
Species
A. victoria
Insert Size (bp)
720
Mutation
N/A
PromoterCAG

Cloning Information

Cloning methodRestriction Enzyme
5′ cloning siteBamHI(not destroyed)
3′ cloning siteEcoRI(not destroyed)
5′ sequencing primeraaagagacagcaaccagg
3′ sequencing primercggcttctggcgtgtgac

Resource Information

Supplemental Documents
- pAAV-CAG-GFP.gb
Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
Industry Terms
- Not Available to Industry
Articles Citing this Plasmid
- 12 References

Depositor Comments

PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Information for AAV1 (Catalog # 37825-AAV1)(Back to top)

Purpose

Ready-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV1
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV1 trial size (Catalog # 37825-AAV1.T)(Back to top)

Purpose

Ready-to-use AAV1 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV1
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 (Catalog # 37825-AAV2)(Back to top)

Purpose

Ready-to-use AAV2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV2
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 trial size (Catalog # 37825-AAV2.T)(Back to top)

Purpose

Ready-to-use AAV2 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV2
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 (Catalog # 37825-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 trial size (Catalog # 37825-AAV5.T)(Back to top)

Purpose

Ready-to-use AAV5 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 (Catalog # 37825-AAV8)(Back to top)

Purpose

Ready-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 trial size (Catalog # 37825-AAV8.T)(Back to top)

Purpose

Ready-to-use AAV8 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 (Catalog # 37825-AAV9)(Back to top)

Purpose

Ready-to-use AAV9 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 trial size (Catalog # 37825-AAV9.T)(Back to top)

Purpose

Ready-to-use AAV9 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV Retrograde (Catalog # 37825-AAVrg)(Back to top)

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Data submitted about 37825-AAVrg by requesting scientist(s):

Data 1: Guinea pig, Brain parenchyma

Information for AAV Retrograde trial size (Catalog # 37825-AAVrg.T)(Back to top)

Purpose

Ready-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Information for AAV PHP.eB (Catalog # 37825-PHPeB)(Back to top)

Purpose

Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
TiterEnter the titer here≥ 1×10¹³ vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, PHP.eB cap gene pUCmini-iCAP-PHP.eB (plasmid #103005)
BufferPBS + 0.001% Pluronic F-68
SerotypePHPeB (plasmid #103005)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Citation Information: When using the PHP.eB serotype in future publications, please acknowledge Viviana Gradinaru and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.

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稳定细胞株筛选

2018-12-26

一、目的要求了解愈伤组织再分化原理，学习诱导愈伤组织分化的方法。二、基本原理愈伤组织在离体培养过程中，组织和细胞的潜在发育能力可以在某种程度上得到表达，伴随着反复的细胞分裂，又开始新的分化。将脱分化的细胞团或组织经重新分化而产生出新的具有特定结构和功能的组织或器官的一查看更多>

骨髓间充质干细胞向肝细胞的体外诱导分化

2018-12-26

1.常用的体外诱导方法是在体外培养的成体干细胞中加人入诱导因子。诱导因子包括各种能影响细胞分化的物质，如血清、糖分、维生素、以及各种蛋白因子等。2.日本学者Oh等首先报道，高浓度肝细胞生长因子(HGF)体外诱导大鼠骨髓细胞分化为肝细胞，并表达CK8, CK18, ALB等。3.2002年，Schwartz等将大鼠、小查看更多>

kementec代理_自产产品

2021-09-03

　　长生不老一直是人类追求的理想目标，但是无论用什么办法，生老病死仍然无情地降临到每个人身上。随着年龄增长，体内组织器官会发生退行性变化，表现为机体活动能力下降、环境适应能力降低、体内代谢平衡失调等一系列衰老现象。衰老是不可抗拒的自然规律，任何人都不能幸免。你知道衰老是怎么发生的吗？　　有关衰老发生的机制，细胞学说认为，人体衰老首先是因为细胞形态结构发生改变，然后引起机体生理功能逐渐衰退。衰老细胞通常会出现水分减少、脂褐素积累、酶活性降... 查看更多>

有没有有了解greenfield这个牌子吉他的童鞋啊_吉他吧_

2021-07-22

据美国CDC数据显示，在美国每隔43秒就会有人心脏病发作；如今，人群中心肌梗死和心力衰竭的高发病率和临床疗效的有限性催生着人们对干细胞疗法的厚望，目前全球已有数千名患者接受了成体干细胞的治疗。日前，来自日本的科学家使用一只猕猴干细胞培育出的心肌细胞成功修复了其它五只猴子的破损心脏，这一研究突破就表明未来科学家或许能够借助捐赠的干细胞让心脏病患者的器官得以再生，而且这种方法还能够明显减少个体干细胞治疗所浪费的时间和费用。那么干细胞疗法查看更多>

日本NIPPONMUKI无机防虫过滤器 DS60031REA20BC0 新品推荐

2017-12-12

2018年6月7日-苏州蚂蚁淘实验试剂有限公司amsbio专业代理,AMSBIO成立于1987年,公司由艾伦和亚历克斯联合创立,总部位于西班牙马德里,AMSBIO在意大利,瑞士... 查看更多>

10x Genomics 单细胞转录组测序_研究

2017-09-11

导读本研究基于10x Genomics微流体芯片技术，采用单细胞转录组测序，较好的在转录水平上描述了多能性细胞培养中体现出的细胞态异质性。根据细胞不同的多能状态，研究人员将18,787个WTC CRISPRi人诱导多能干细胞分为四个亚群，并确定了定义每个亚群的新基因和通路，开发出一个用于单细胞精确亚群分类的多基因预测模型。该研究为我们提供了目前最大的关于未分化的人诱导多能干细胞的单细胞转录表达谱数据库，包含5个生物复制子的18,787个... 查看更多>

多聚甲醛戊二醛固定液说明书 Coolaber

2018-01-11

这一创新研究由美国北卡州立大学／北卡教堂山分校的程柯教授带领团队完成。他们研发出血小板纳米颗粒，用于修饰心脏干细胞，指引干细胞靶向受损心脏，并停留在损伤区域，从而增强干细胞的修复能力。相关研究成果发表在《Nature Biomedical Engineering》期刊。利用血小板的靶向功能程柯表示：“血小板就是一把双刃剑。当损伤发生时，血小板可以聚集在损伤部位，有些研究发现血小板甚至可以诱导内源性干细胞募集在损伤区域。但是，血小板又是引查看更多>

谁在做这个实验:流式细胞术间接免疫荧光标记的样品制备实验实验方 ...

2021-07-20

澳大利亚沃尔特和伊莱扎学院（WEHI）的研究人员利用先进的细胞学、生物信息学和图像技术揭示了乳房中一种长寿命的干细胞类型，这种干细胞与孕期乳腺生长有关。这种新发现的干细胞能够应答卵巢激素——孕激素和雌激素，还与一种乳腺癌的发生风险有关。相关研究结果发表在国际学术期刊Natue Cell Biology上。文章作者Dr. Nai Yang Fu表示他们基于之前关于乳腺干细胞的一些研究，发现了具有不同功能的干细胞亚群。“我们观察了在这些干细查看更多>

阿伯丁大学_

2018-07-11

广州创赛生物医用材料有限公司在发布的胚胎干细胞体外培养与定向诱导分化供应信息，浏览与胚胎干细胞体外培养与定向诱导分化相关的产品或在搜索更多与胚胎干细胞体外培养与定向诱导分化相关的内容。查看更多>

我国关于抗生素的政策规划兽药常识

2017-07-28

　　整形外科从本质上说是对外形的追求。而外形意味着有(或者没有)体积。如果体积不足，就必须寻求其他方法来弥补：或者在缺损区域填充材料，或者通过转移身体他处的组织修复患处。如果体积过剩，可选择去除一部分组织。　　同医学界的其他许多领域一样，整形外科学也应用同样的理念，即去除多余的组织，修复或再生缺失的组织，尤其是后者，整形外科医生一直在探寻利用自身机体组织作为修复材料来源，补其所缺的可能性。　　当壁虎躲避捕猎者而自断其尾，逃之夭夭，其断尾... 查看更多>

兔成骨细胞与骨髓基质干细胞混合培养的实验研究

2018-12-26

对骨髓基质干细胞进行改造,使其在保持快速生长的同时向成骨细胞方向分化是目前国内外研究的热点。实验发现,成熟的成骨细胞可以通过细胞间的直接接触作用促进骨髓基质干细胞增殖[ 1 ] ,此外成骨细胞可产生多种生长因子促进其骨髓基质干细胞的增殖、分化[ 2 ] 。由于骨髓基质干细胞通过诱导向成骨查看更多>

最新干细胞临床试验研究进展

2017-08-22

近年来，科学家们在干细胞临床试验研究上取得了多项重磅级研究成果，本文中小编就对相关研究进展进行了整理盘点，分享给各位！与大家一起学习！查看更多>

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小鼠胚胎干细胞培养几天后贴壁不牢换液容易飘走怎么办? 123

实验先呆2017-08-30

第一次养胚胎干细胞，复苏的时候复苏到六孔板中的其中一个孔中，结果发现细胞很多，于是换了两个6cm皿养，结果越养越少。很纳闷。请教了几位蚂蚁淘的前辈们，于是乎有几个问题还是在心中，希望还能在这里能得到答复。

1。培养细胞的时候是一定要在六孔板中吗？那一般复苏细胞的时候复苏到几个孔中呢?

2.细胞的密度是大概多少呢?

3.我用的基质胶，说明书上是1;80，结果在孵箱放了一小时后，发现没有想象的胶凝固的样子，没有像果冻那样。反而还是液体状的，这是能用还是不能用呢？

4.如果基质胶一开始就铺好，等到传代的时候再铺，中间每天都在换液，不容易污染吗?

5。刚复苏的细胞，一直漂浮在培养液里，是慢慢地细胞才会贴在基质胶上么，然后才会慢慢在基质胶上生长么？

脐带间充质干细胞可以直接从干细胞公司买来自己移植吗123

BSHT_4562017-08-20

各位大神，我将培养后的间充质干先液氮冻存了，现在想提rna，可以直接离心做吗？我看有人说要在冰上融化，融化后立即四度离心，离心之后就可以按正常步骤提取了。可是我冻存细胞的时候里面加入了dmso血清和培养基，不需要pbs洗一下再提吗？

实验室里长出的干细胞培育“人造肉”,你敢吃吗? 中国江苏网123

hgx08092017-08-21

小弟养脂肪干细胞，不知道那一步出问题长出的毛骨悚然的东西，求各位大神鉴定一下，给予一些指导。不甚感激

干细胞的名词解释 123

2017-09-30

干细胞是一种尚未分化、尚不成熟、具有自我复制能力、具有再生各种组织器官、具有人体的潜在功能的多潜能细胞。增殖和分化潜能是干细胞的主要潜能，干细胞可以分为全能干细胞、多能干细胞和单能干细胞。
全能干细胞具有能发育成各种组织器官的完整个体的潜能的细胞，如胚胎干细胞。多能干细胞没有发育成完整个体的能力，但具有分化出多种细胞组织的能力，主要在器官再生、修复和疾病治疗方面极具应用价值，多能干细胞还在研究当中，过去认为多能干细胞只能从人胚胎中获得，现在已能用体细胞转化为多能干细胞。
单能干细胞只能向一种类型或密切相关的两种类型的细胞分化，如上皮组织基底层的干细胞、肌肉中的成肌细胞。

哪位前辈可以上传一下C57小鼠骨髓间充质干细胞的具体培养步骤 ...123

晴天娃娃96072017-08-27

我是个实验新手，看了很多园子里关于小鼠骨髓间充质干细胞的帖子，好多人都说不好养，希望哪位仁兄能上传一下具体实验步骤，真的万分感谢

干细胞的分类及应用 123

2017-09-30

干细胞的含义：干细胞是一类具有自我更新能力的多潜能细胞，即干细胞保持未定向分化状态和具有增殖能力，在合适的条件或给予合适的信号，它可以分化成多种功能细胞或组织器官，医学界称其为“万用细胞”，也有人通俗而形象地称其为“干什么都行的细胞”。在一定条件下，它可以分化成多种功能细胞。
　　干细胞的分类：
　　一）根据个体发育过程中出现的先后次序不同，干细胞又可分为胚胎干细胞和成体干细胞。
　　(1)胚胎干细胞，它是一种高度未分化细胞。它具有发育的全能性，能分化出成体动物的所有组织和器官，包括生殖细胞。
　　(2)成体干细胞，它是存在于成年动物的许多组织和器官，比如表皮和造血系统，具有修复和再生的能力的细胞。在特定条件下，成体干细胞或者产生新的干细胞，或者按一定的程序分化，形成新的功能细胞，从而使组织和器官保持生长和衰退的动态平衡。
　　二）按分化潜能的大小，干细胞还可分为全能性干细胞、多能性干细胞、单能干细胞等三种类型：
　　(1)全能性干细胞，它具有形成完整个体的分化潜能。如胚胎干细胞(简称ES细胞)，具有与早期胚胎细胞相似的形态特征和很强的分化能力，可以无限增殖并分化成为全身200多种细胞类型，进一步形成机体的所有组织、器官。人类的全能干细胞可以分化成人体的各种细胞，这些分化出的细胞构成人体的各种组织和器官，最终发育成一个完整的人。人类的精子和卵子结合后形成受精卵，这个受精卵就是一个最初始的全能干细胞。
　　(2)多能性干细胞，这种干细胞具有分化出多种细胞组织的潜能，但却失去了发育成完整个体的能力，发育潜能受到一定的限制，骨髓多能造血干细胞是典型的例子，它可分化出至少十二种血细胞，但不能分化出造血系统以外的其它细胞。
　　(3)单能干细胞(也称专能、偏能干细胞)，这类干细胞只能向一种类型或密切相关的两种类型的细胞分化，如上皮组织基底层的干细胞、肌肉中的成肌细胞或叫卫星细胞。

国内外干细胞研究现状及趋势综述123

伯伯你好1232017-09-30

由于干细胞研究的关键技术获得突破仅有十几年时间，而中国本身的研究水平并不差，所以中国和西方国家几乎处在同一起跑线上，在这一领域，中国最有可能完成科学上的原创性贡献。事实上，中国的干细胞研究和应用确实具备了一定的基础，早在上世纪60年代，中国就开始了骨髓移植研究；到上世纪70年代末80年代初，临床骨髓移植治疗血液病陆续开展；上世纪90年代以来，除骨髓移植外，外周血和脐血干细胞移植也逐步普及应用于治疗血液病和肿瘤。2009年，国家生物产业基地——干细胞与再生医学产业化项目在江苏省泰州市中国医药城正式开工建设，江苏省干细胞与生物治疗公共技术服务平台，江苏省干细胞库脐带间充质干细胞储存业务同时启动。该产业化项目由深圳市北科生物科技有限公司全资子公司——江苏省北科生物科技有限公司承建并负责全面运营。国家生物产业基地——干细胞与再生医学产业化项目，是在江苏省干细胞库的基础上进行产业化的扩大建设，包括干细胞技术转化中心、检测中心、江苏省干细胞库二期工程、干细胞临床技术服务中心四大中心，是集干细胞技术转化、干细胞储存和应用于一体的综合性干细胞产业化基地。其中江苏省干细胞库二期工程将严格按照 GMP 及美国 GTP 标准进行建设，建成后设计储存量将达到100万份，成为亚洲最大、世界一流的综合性干细胞库。该库将会进一步扩大脐带间充质干细胞等多种来源的干细胞保存业务，并在iPS细胞方面持续投入大量的研发力量，为干细胞应用于临床、治疗多种难治性疾病提供高质量的干细胞源，为广大民众的健康提供强大的保障。

[经验分享]人牙周膜干细胞培养口腔论坛123

nadiaa2021-07-25

上了研究生就开始养牙周膜干细胞，经历了小半年无数次的失败，可能有快100颗牙齿了吧，那些个无助和绝望...

肿瘤干细胞培养遇到问题了,望众多虫友不吝赐教! 生物科学...123

Anya-蘇2021-07-21

低黏附培养板，每孔接种5000个细胞，无血清培养，但是第二天去看，感觉细胞都抱在一起死了，求哪位前辈帮忙看看，这是怎么回事

干细胞的特征是什么?_39健康问答_123

奶茶kx2弴2017-09-30

干细胞有以下特点：
1. 干细胞本身不是终末分化细胞(即干细胞不是处于分化途径的终端)；
2. 干细胞能无限增殖分裂；
3. 干细胞可连续分裂几代，也可在较长时间内处于静止状态；
4. 干细胞分裂产生的于细胞只能在两种途径中迭择其一或保持亲代特征，仍作为干细胞；或不可逆地向终末分化。
由
于细胞质中的调节分化蛋白不均匀地分配，使得一个子细胞不可逆地走向分化的终端成为功能专一的分化细胞；另一个保持亲代的特征，仍作为干细胞保留下来。分
化细胞的数目受分化前干细胞的数目和分裂次数的控制，可以说，干细胞是具多向潜能和自我更新特点的增殖速度较缓慢的细胞

多能干细胞的来源 123

2017-09-30

多能干细胞的简单获得人类多能性干细胞系的建立有两个来源，其方法与以往在动物模型中建立的方法相同。（1）在Dr. Thomson进行的工作中，他从人类胚胎的囊胚期内细胞群中直接分离多能干细胞。Dr. Thomson从IVF（体外受精）临床实验室得到胚胎，这些胚胎是不育症临床治疗不需要的，用于繁殖，而非研究目的。从捐献者夫妇处获得知情同意书后，Dr. Thomson分离了内细胞群，将这些细胞进行培养，产生一个多能性干细胞系。
（2）与此相反，Dr. Gearheart从终止妊娠的胎儿组织中分离出多能性干细胞。捐献者自行决定了终止妊娠，从他们那儿获得了知情同意书后，Dr. Gearheart从原本要发育成睾丸或卵巢的胎儿部位取得细胞。尽管Dr. Thomson 实验室和Dr. Gearheart实验室使用的细胞系来源不同，但发育成熟的细胞看起来非常相似。
体细胞核转移（SCNT）是得到多能性干细胞的另一种途径。在SCNT的动物研究中，研究者将一个正常的动物卵细胞去除细胞核（含染色体的细胞结构）。存留在卵细胞内的物质含营养成分和对胚胎发育非常重要的能量物质。而后，在非常精细调控的实验室条件下，将单个体细胞——除卵细胞或精子细胞之外的任一种细胞——与除去核卵细胞放在一起，使两者相融合。融合细胞以及其子细胞具有发育成一个完整个体的潜能，因此是全能性的。正如图I所示，这些全能性细胞不久将形成胚囊，从理论上来说，可利用胚囊的内细胞群来建立多能性干细胞系。实际上，任何一种可生成人类胚囊细胞的方法都有可能成为人体多能性干细胞的来源。

DNA转染(Entranster)与脂肪来源干细胞和心肌梗死研究【生命科学吧】_百...123

ding2000yj2021-07-26

心肌梗死（Myocardialinfarction，MI）特征是心脏供血减少和心肌功能减弱，成人心脏的自我再生能力有限，使得心肌梗死的状况雪上加霜。由于干细胞具有自我更新复制能力和分化潜能，因此干细胞移植是使心脏组织再生和增强心脏功能的新的方法。最近的研究表明脂肪组织来源干细胞（ADSCs），即从脂肪组织分离的多能干细胞，具有广泛的分化潜能，可分化为心肌细胞。现分享一篇DNA转染（Entranster-H4000）与脂肪来源干细胞和心肌梗死研究的文献，以供参考。

Real-timetrackingofADIposetissue-derivedstemcellswithinjectablescaffoldsintheinfarctedheart.pdf(866.54k)