- Description
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Description
Details
Description:Mouse monoclonal antibody to human interleukin 8 (IL-8)
Purification: Protein G affinity purified
Product Type:Primary antibody
Target Protein:Human IL-8
Immunogen:Purified recombinant human IL-8
Fusion Myeloma:P3X63Ag8.653
Specificity:This antibody recognizes both recombinant and native human IL-8.
Species Reactivity: Human, others not tested.
Cross-reactivity:This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Host / Isotype: Mouse, IgG1 Kappa
Formulation: Lyophilized in 0.01M PBS, pH 7.0.
Reconstitution:Double distilled water is recommended and to adjust the final concentration to 1.00 mg/mL.
Storage: Store at -20oC
Research Area:Cytokine, interleukin and chemotaxis
Background:
Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8"s primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.
Applications:
ELISA: React with human IL-8.
Neutralizing: In chemotaxis assay, the antibody inhibited 97 % of the chemotactic activity of IL-8 on RB/293 cell using 5mg/ml of MAb and 10ng/ml of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, and had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating that the neutralizing activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.
Western Blot: The antibody when used at 0.02-0.1mg/ml concentration, will allow visualization of 100ng/lane of recombinant human IL-8. Figure (see image): Western blot analysis of recombinant human IL-8 using clone I8-61.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Human IL-8, I8-61 |
|---|---|
| Application | EIA, NT, WB |
| Size | 0.1 mg |
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由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株，就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞
3.收集病毒液
4.用病毒液感染靶细胞
5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法
6.获得稳转株
建立稳定细胞株,一般是根据不同基因载体中所含有的抗性标志选用相应的药物对靶...专注整体实验·服务生命科学已开展了数千个实验外包项目
如果经费充足的话可以找公司包装病毒，如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。
