The CELLvo Matrix is an Extracellular Matrix (ECM) of proteins synthesized in vitro by bone marrow stromal cells. This product is composed of more than 150 proteins that were secreted and assembled by bone marrow cells during the production of the CELLvo Matrix. The final product is cell free with only the ECM attached to the surface of the culture vessel. This cell culture substrate provides a native three-dimensional microenvironment, which can be used for rapid and reliable cell expansion of high quality Mesenchymal Stem Cells (MSCs).
CELLvo Matrix Spec Sheet
Frequently Asked Questions
Technical Bulletin: Optimized Isolation and Growth of Mesenchymal Stem Cells - The CELLvo Matrix
National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria thatgrow aerobically,5th ed. A 查看更多>
Analysis of ES Cell Clones by Mini-SouthernInitial Cloning1.Aspirate selective media off the plates containing colonies of interest. Wash the plates twice wit 查看更多>
Media and Solutions Required for Routine ES Cell Culture: Version 1 (PMCI) Media and Solutions Required for Routine ES Cell Culture: Version 2 (PMCI) Media for 查看更多>
What you need: E11-12 mouse embryos DMEM with 5% fetal bovine serum petri dishes for dissections and washes Tyrode-Ringer"s solution, pH 7.6-7.7 (recipe below) 查看更多>
Preparation of Broth and Plates, etc.[Use ddH2O 17 m* or better ]1) LB Broth Make 16 gm of LB Broth Base (Gibco #M27800C) up to 800 ml in ddH2O. Swirl to disso 查看更多>
This document assumes that you have a mouse IgG monoclonal antibody directed to the antigen you wish to quantitate and that you have fluorescein labeled and fu 查看更多>
Cell are normally passaged every 2-3 days, this is important to avoid differentiation.Signs of differentiation are:-i) colonies are surrounded by flattened dif 查看更多>