- SynonymIL-17A,Interleukin-17A,CTLA-8,IL-17
- SourceMouse IL-17A, His Tag (CT8-M5240) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1).Predicted N-terminus: HisRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 15.8 kDa. The protein migrates as 18 kDa, 20-23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mouse IL-17A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Mouse IL-17A, His Tag (Cat. No. CT8-M5240) was more than 85% and around 40-55 kDa verified by SEC-MALS.
Immobilized Mouse IL-17A, His Tag (Cat. No. CT8-M5240) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 4-125 ng/mL (QC tested).
Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Mouse IL-17A, His Tag (Cat. No. CT8-M5240) with an affinity constant of 20.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Mouse IL-17A, His Tag (Cat. No. CT8-M5240) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 2.132-5.005 ng/mL.
- BackgroundInterleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
- References
- (1)Yao Z., et al.,1995, J. Immunol. 155:5483-5486.
- (2)Gerhardt S., et al., 2009, J. Mol. Biol. 394:905-921.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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用于酵母繁殖和筛选的培养基
SD是含有盐类、微量元素、维生素、氮源(不含氨基酸的酵母氮碱)和葡萄糖的合成基本培养基。
注意：氮源(不含氨基酸的酵母氮碱)是这种培养基用于筛选的关键，LB，YPD这种复合培养基因为用到了酵母提取物，蛋白胨，基本是氮源全营养的，肯定是不能用来做氮源筛选的
HT培养基添加剂(HT Supplement)为粉剂，经照射灭菌。每个包装可配制成10ml浓缩液（50X），用于杂交瘤细胞的筛选。
要培养293A细胞，请教各位大神，配制好的完全培养基4度能放置多久？
各位大神你们好：
我们是医疗器械工厂，只需配制环境监测和水检测的培养基，但是根据2015版药典规定，1105，141页有一句
而在9203，289页
“商品化的成品培养基除了应附有处方和使用说明外，还应注明有效期、贮藏条件、适用性检查试验的质控菌和用途”。
是否可以理解为只要供应商提供以上信息，使用厂家就可以不用做培养基适用性试验了？
如果购买的商品化的成品培养基不做培养基适用性试验，那么医疗器械的微生物检验将大大减少工作量了。
