![SMOBIO/[DM3260] FluoroBand™ 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder, 500 μl/500 μl/DM3260](images/SMOBIO/image.jpg)
Description
The DM3260 FluoroBand™ 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM3260 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 10,000 bp
Concentration
87 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light Room temperature for 6 months4°C for 12 months -20°C for 24 months
Specification
Cat. No. | DM3260 |
Series Name | FluoroBand™ |
Product Size | 500 μl |
Size Range | 100 – 10000 bp |
Band Number | 19 |
Tracking Dye | Orange G, Bromophenol blue and Xylene cyanol FF |
Enhanced Band | 500, 1500 and 3000 bp |
Manual
Manual_DM3260_FluoroBand™ 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder
SDS
SDS_DM3260
Are the DNA markers/ladders produced by SMOBIO sufficient in quantity?
Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA.
Can I combine non-fluorescent markers and fluorescent loading dye (ex. DL5000) to replace FluoroBand™ fluorescent DNA ladders?
SMOBIO’s fluorescent DNA ladders is better in intensity and accuracy than a fluorescent marker produced by mixing non-fluorescent one with reagents containing fluorescent DNA dyes such as DL5000. In another aspect, DL5000 is designed for quick screening, not intended for preparation of DNA ladders which needs careful calibration. Therefore we suggest using our fluorescent DNA ladders directly.
Will FluoroBand™ fluorescent DNA ladders gradually lose fluorescent intensity?
The fluorescent signals of a fluorescent DNA ladder might be reduced if frequently exposed to light for a long term. Therefore, we suggest keeping fluorescent DNA ladders from exposure to light.
Can fluorescent DNA markers be visualized when illuminated with UV light?
Yes, it is possible to view the fluorescent signals under blue light and UV light.
The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins
Chih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971
PMCID: PMC5790263
Transposable elements generate population-specific insertional patterns and allelic variation in genes of wild emmer wheat (Triticum turgidum ssp. dicoccoides)
Katherine Domb, Danielle Keidar, Beery Yaakov, Vadim Khasdan, Khalil Kashkush BMC Plant Biol. 2017; 17: 175. Published online 2017 Oct 27. doi: 10.1186/s12870-017-1134-z
PMCID: PMC5659041
FluoroBand™ DNA Ladder series
ExcelBand™ DNA Ladder series
FluoroDye™ DNA Fluorescent Loading Dye
Excellent for premix with DNA sample
Sensitivity up to 0.14 ng DNA
Safety dye
Convenience - monitor the electrophoresis in real-time
FluoroVue™ Nucleic Acid Gel Stain
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
B-BOX™ Blue Light LED Epi-illuminator
470 nm long wavelength
Improved cloning efficiency
Compact, light-weight, and portable (less than 1 kg)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation
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（一）按成分不同划分
1、天然 培养基 (complex medium) 这类培养基含有化学成分还不清楚或化学成分不恒定的天然有机物，也称非化学限定培养基(chemically undefined medium)。牛肉膏蛋白胨培养基和麦芽汁培养基就属于此类。基因克隆技术中常用的LB(Luria—Bertani)培养基也是一种天然培养基，其组成见表5.9。
牛肉浸膏、蛋白胨及酵母浸膏的来源及主要成分
营养物质 牛肉浸膏
来 源 瘦牛肉组织浸出汁浓缩而成的膏状物质
主要成分 富含水溶性糖类、有机氮化合物、维生素、盐等
营养物质 蛋白胨
来 源 将肉、酪素或明胶用酸或蛋白酶水解后干燥而成
主要成分 富含有机氮化合物、也含有一些维生素和糖类的粉末状物质
营养物质 酵母浸膏
来 源 酵母细胞的水溶性提取物浓缩而成的膏状物质
主要成分 富含B类维生素，也含有有机氮化合物和糖类
常用的天然有机营养物质包括牛肉浸膏、蛋白胨、酵母浸膏(表5.10)、豆芽汁、玉米粉、土壤浸液、麸皮、牛奶、血清、稻草浸汁、羽毛浸汁、胡萝卜汁、椰子汁等，嗜粪微生物(coprophilous microorganisms)可以利用粪水作为营养物质。天然培养基成本较低，除在实验室经常使用外，也适于用来进行工业上大规模的微生物发酵生产。
2、合成培养基(synthic medium)是由化学成分完全了解的物质配制而成的培养基，也称化学限定培养基(chemically defined medium)，高氏I号培养基和查氏培养基就属于此种类型。配制合成培养基时重复性强，但与天然培养基相比其成本较高，微生物在其中生长速度较慢，一般适于在实验室用来进行有关微 生物营养需求、代谢、分类鉴定、生物量测定、菌种选育及遗传分析等方面的研究工作。
（二）根据物理状态划分
根据培养基中凝固剂的有无及含量的多少，可将培养基划分为固体培养基、半固体培养基和液体培养基三种类型。
1、固体培养基(so1id medium)
在液体培养基中加入一定量凝固剂，使其成为固体状态即为固体培养基。理想的凝固剂应具备以下条件：①不被所培养的微生物分解利用；②在微生物生长的温度范围内保持固体状态，在培养嗜热细菌时，由于高温容易引起培养基液化，通常在培养基中适当增加凝固剂来解决这一问题；③凝固剂凝固点温度不能太低，否则将不利于微生物的生长；④凝固剂对所培养的微生物无毒害作用；⑤凝固剂在灭菌过程中不会被破坏；⑥透明度好，粘着力强；⑦配制方便且价格低廉。常用的凝固剂有琼脂(agar)、明胶(gelatain)和硅胶(silica gel)。表5.11列出琼脂和明胶的一些主要特征。
对绝大多数微生物而言，琼脂是最理想的凝固剂，琼脂是由藻类(海产石花菜)中提取的一种高度分支的复杂多糖；明胶是由胶原蛋白制备得到的产物，是最早用来作为凝固剂的物质，但由于其凝固点太低，而且某些细菌和许多真菌产生的非特异性胞外蛋白酶以及梭菌产生的特异性胶原酶都能液化明胶，目前已较少作为凝固剂；硅胶是由无机的硅酸钠(Na2SO3)及硅酸钾(K2SiO3)被盐酸及硫酸中和时凝聚而成的胶体，它不含有机物，适合配制分离与培养自养型微生物的培养基。
HT培养基添加剂(HT Supplement)为粉剂，经照射灭菌。每个包装可配制成10ml浓缩液（50X），用于杂交瘤细胞的筛选。
大家好，我是一个质粒小菜鸟，最近遇到个奇怪的事情，希望大家帮忙看看，究竟出了什么问题。
我构建了一个氨苄抗性的质粒，这个质粒在固体培养基里长出了单菌落，但是我挑菌在液体培养基里无法扩增出来。固体培养基和液体培养基一起配置的，氨苄浓度一致，唯一不同的是固体培养基里加入了琼脂。为了防止是氨苄的问题，我把这个固体培养基上的菌落挑划了新的板，依然能长出菌落。而同一次配置的液体培养基，也能扩增其他质粒，所以我实在想不出是什么其他问题，希望大家帮帮忙。
各位大神你们好：
我们是医疗器械工厂，只需配制环境监测和水检测的培养基，但是根据2015版药典规定，1105，141页有一句
而在9203，289页
“商品化的成品培养基除了应附有处方和使用说明外，还应注明有效期、贮藏条件、适用性检查试验的质控菌和用途”。
是否可以理解为只要供应商提供以上信息，使用厂家就可以不用做培养基适用性试验了？
如果购买的商品化的成品培养基不做培养基适用性试验，那么医疗器械的微生物检验将大大减少工作量了。
用于酵母繁殖和筛选的培养基
SD是含有盐类、微量元素、维生素、氮源(不含氨基酸的酵母氮碱)和葡萄糖的合成基本培养基。
注意：氮源(不含氨基酸的酵母氮碱)是这种培养基用于筛选的关键，LB，YPD这种复合培养基因为用到了酵母提取物，蛋白胨，基本是氮源全营养的，肯定是不能用来做氮源筛选的
