ProductOverview:
Anewrobustandversatileserum-freemediumformulationforhighandlowdensityNHEK-NormalHumanEpidermalKeratinocytecultures
KGM-Gold™KeratinocyteGrowthMediumisdesignedtosupportbothclonalgrowthandhighdensitykeratinocyteproliferation.ThemediumsupportssuperiorgrowthandmorphologyofkeratinocytesisolatedinKGM-2™(datanotshown),KGM™(datanotshown)andCompetitorA"sMedium(datashownbelow).
FurThermore,qualitycontrolatLonzarequiresthatkeratinocytespassstrictmorphologicalcriteriaspecifyingtheminimalpresenceofenlargedandvacuolatedcells,enablingLonzakeratinocytestobeusedbytheresearcherfor≥15PopulationDoublings.
Adultandneonatalkeratinocytesarealsoavailable.
RecommendedSubculturingReagents: ReagentPackTMSubcultureReagents
Inaside-by-sidecomparisonwithamajorcompetitor’sformulation,culturesmaintainedinKGM-Gold™KeratinocyteGrowthMediumhadamorehomogeneousphenotypeconsistingofsmallrefractilecellswithminimalenlargementandvacuolization.TheABIlityofKGM-Gold™MediumtopromotethehealthofNHEK-NormalHumanEpidermalKeratinocytecellspositivelyinfluenceskeratinocyteLifespan.
Figure4A: GrowthofLonza’sadultkeratinocytesgrowninKGM-Gold™GrowthMediumcomparedtoCompetitorA’sadultkeratinocytesintheirmedium.
 | Lonza’sadultkeratinocytesgrowninKGM-Gold™outperformedcompetitorA’sadultkeratinocytesgrownintheirownmediumingrowthrate(Figure-4A),andmorphology(Figure-4B)uptoandevenbeyond15populationdoublings.ThisdatashowsthatalthoughcompetitorAclaimsextendedlifespanoftheirkeratinocytesintheirownmedium,thehealthandmorphologyoftheseadultkeratinocytesispoorbeyond13-15populationdoublings,renderingtheculturesunusableforfurtherexperimentation. |
Figure4B: MorphologyofLonza’sadultkeratinocytesgrowninKGM-Gold™GrowthMediumcomparedtoCompetitorA’sadultkeratinocytesintheirmedium.
| Lonza"sAdultKeratinocytes in KGM-Gold™Medium | CompetiorA"sAdultKeratinocytes inTheirMedium | ||
 Day13,15TPD |
Day25,17TPD | Findmoredatainourwhitepaper:"ASerum-freeMediumforNormalHumanEpidermalKeratinocytes(NHEK)"
Registertodownload | |
Day20,19TPD |
Day32,19TPD | ||
ThegrowthperformanceandlongevityofCompetitorA"sadultkeratinocytesgrowninCompetitorA"sMediumwerecomparedwithLonza’sadultkeratinocytesgrowninKGM-Gold™GrowthMedium. AlthoughCompetitorAclaims≥25totalpopulationdoublingsfortheiradultkeratinocytes,actualresultsshowthatthislotbarelyobtained19PD. NotethepresenceofseveralenlargedcellsinPassage2inCompetitor’sMedium. Attheendofpassage4(15PD)Lonza’sadultkeratinocytesconsistofmostlysmallproliferativecells.
New!PurchaseourAdultKeratinocyteCellCultureKit(catalogno.192627B)andgetadiscountedpriceoncellsandmedia. Thekitconsistsofacryopreservedampuleofadultkeratinocytes(catalogno.192627)andKGM™-GoldBulletKit™GrowthMedia(catalogno.192060)
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用于酵母繁殖和筛选的培养基
SD是含有盐类、微量元素、维生素、氮源(不含氨基酸的酵母氮碱)和葡萄糖的合成基本培养基。
注意：氮源(不含氨基酸的酵母氮碱)是这种培养基用于筛选的关键，LB，YPD这种复合培养基因为用到了酵母提取物，蛋白胨，基本是氮源全营养的，肯定是不能用来做氮源筛选的
HT培养基添加剂(HT Supplement)为粉剂，经照射灭菌。每个包装可配制成10ml浓缩液（50X），用于杂交瘤细胞的筛选。
要培养293A细胞，请教各位大神，配制好的完全培养基4度能放置多久？
各位大神你们好：
我们是医疗器械工厂，只需配制环境监测和水检测的培养基，但是根据2015版药典规定，1105，141页有一句
而在9203，289页
“商品化的成品培养基除了应附有处方和使用说明外，还应注明有效期、贮藏条件、适用性检查试验的质控菌和用途”。
是否可以理解为只要供应商提供以上信息，使用厂家就可以不用做培养基适用性试验了？
如果购买的商品化的成品培养基不做培养基适用性试验，那么医疗器械的微生物检验将大大减少工作量了。
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