BSA reagent pH7 grade is a highly purified, lyophilized powder, it has a pH of ≈7 when dissolved in water.
| Specification - Bovine Serum Albumin - Reagent Grade pH7 (BSA-7) | |
| Product | Bovine Serum Albumin- Reagent Grade pH 7 |
| Catalogue No. | BSA7 |
| Certificate of Suitability | Australia: R1-CEP 2005-191-Rev 00New Zealand: R1-CEP 2003-205-Rev 00 |
| Source | Bovine plasma from healthy animals inspected ante and post mortem by New Zealand or Australian Government veterinarians and passed as fit for human consumption |
| Collection/Processing Method | Cohn Fraction V Purified by heat shock treatment with charcoal treatment and diafiltrarion. |
| Description | Off white to pale green / yellow lyophilised powder |
| Stability | Stable for a minimum of 5 years under correct storage conditions |
| Storage | Store in sealed containers with a desiccant under dry conditions between 2 - 8°C |
| Analysis | Method | Range |
| pH | pH meter | 6.5 - 7.2 |
| Albumin | Gel Electrophoresis | ≥ 98% |
| Protein | LECO CF003.1 (dry basis) | ≥ 96% |
| Moisture Content | Oven Moisture Content | ≤ 5.0% |
| Chloride | Coulometric | ≤ 0.15% |
| Calcium | ICP-OES | ≤ 0.05% |
| Iron | ICP-OES | ≤ 0.002% |
| Magnesium | ICP-OES | ≤ 0.005% |
| Phosphorus, inorganic | Coulometric | ≤ 0.005% |
| Potassium | ICP-OES | ≤ 0.015% |
| Sodium | ICP-OES | ≤ 0.08% |
| Lead | ICP-OES | ≤ 0.003% |
| Ash Content | Gravimetric | ≤ 2.5% |
| Optical Density | Abs 405nm at 60mg/ml sol | ≤ 0.2 |
| Solubility | 60mg/ml sol. | Soluble |
| Bioburden | Heterotrophic Colony count - pour plate | ≤ 100 CFU/g |
EDQM
The European Directorate for the Quality of Medicines (EDQM) has originated from the European Pharmacopoeia Secretariat which, with the addition of new responsibilities changed its name to the European Department for the Quality of Medicines (EDQM). The EDQM sits alongside the European Medicines Agency (EMEA).
Amongst other activities, the EDQM is responsible for the European Pharmacopeia and the issuing of Certificates of Suitability (CEP).
Certificates of Suitability (CEP) are recognised by all signatory states of the European Pharmacopoeia Convention and by the European Union. Other countries have also chosen to recognise them. In the case of Fetal Bovine Serum and other Bovine Serum a CEP can be used by the manufacturers of Fetal Bovine Serum and other Bovine Serum which is intended for use in the manufacture of pharmaceutical products to demonstrate compliance with the Bovine Serum monographs of the European Pharmacopoeia and the EDQM requirements for substances concerned by TSE risk.
The discovery of Bovine Spongiform Encephalopathy (BSE), which is one of a group of similar infections now referred to as Transmissible Spongiform Encephalopathies (TSEs), and it’s spread over many countries of the European Union as well as its discovery in Canada, the USA and Japan is well known. The European events provided the impetus for the Council of Europe Public Health Committee (CEPHC) to pass Resolution AP-CSP (99) 5 addressing the TSE concerns and creating a Certificate of Suitability (COS) pursuant to Directive 75/318/EEC.
The granting of a CoS to a manufacturer, for a particular product, certifies that the product in question has been assessed for the level of risk of transmission of TSEs, and that the risk level is considered low enough that the product is certified as suitable for use in the manufacture of medicinal products in the European Union.
The process of approval requires the manufacturer to submit a dossier that covers all relevant aspects of the collection of raw material and further processing that is performed to reach the product that is offered to end users. This dossier is detailed and covers the specific material that is collected, the collection method, process validation, testing, traceability, quality systems and an expert review.
Only after this dossier has been examined and approved by two rapporteurs and, if necessary, by a panel of experts appointed by the European Directorate for the Quality of Medicines (EDQM) is the manufacturer granted a COS.
Moregate Biotech has been granted a COS for:
- Fetal Bovine Serum – Australian Origin – CEP2000-187
- Fetal Bovine Serum – New Zealand Origin – CEP2000-188
- Adult Bovine Serum - New Zealand Origin – CEP2001-093
- Adult Bovine Serum (Defibrinated) - New Zealand Origin – CEP2000-174
- Bovine Plasma – New Zealand Origin – CEP2003-199
- Bovine Serum Albumin – manufactured in Australia from New Zealand origin Bovine Plasma – CEP2003-205
- Bovine Plasma – Australian Origin - CEP 2005-192
- Bovine Serum Albumin – manufactured in Australia from Australian origin Bovine Plasma - CEP 2005-191
Copies of the Certificates of Suitability are available upon request
Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
- Not hazardous.
First Aid Measures
Get Medical attention immediately.
Inhalation
If inhaled, remove to fresh air. If breathing becomes difficult, call a physician
Ingestion
If swallowed, give several glasses of water to drink to dilute.
Skin Contact
Wash skin with soap and copious amounts of water.
Eye Contact
Flush with water for at least 15 minutes, lifting upper and lower eyelids occasionally.
Procedures for Personal Precaution
Exercise appropriate precautions to minimize direct contact with skin or eyes.
Methods for Cleaning Up
Mop up
Ventilate area and wash spill site after material pickup is complete
Fire fighting Measures
Extinguishing Media
Water spray. Carbon dioxide, dry chemical powder, or appropriate foam.
Special Risks
Emits toxic fumes under fire conditions
Special Protective Equipment for Firefighters
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
Accidental Release
Procedures for Personal Precaution
Exercise appropriate precautions to minimize direct contact with skin or eyes.
Methods for Cleaning Up
Mop up
Ventilate area and wash spill site after material pickup is complete
Handling and Storage
Handling
Avoid contact with eyes, skin and clothing.
Storage
Keep tightly closed. Store at 2 - 8 °C.
Exposure Controls / Personal Protection
- Wash thoroughly after handling.
- Wear dust mask
- Protective gloves
- Chemical safety goggles
Disposal Considerations
Contact a licensed professional waste disposal service to dispose of this material.
Transport Information
- Non-hazardous for road transport
- Non-hazardous for sea transport
- Non-hazardous for air transport
Note: The above information is believed to be correct, but shall be used as a guide only.
Disclaimer: For pharmaceutical use only.
BOVINE SERUM ALBUMIN
Moregate Bovine Serum Albumin BSA is manufactured from Bovine Plasma of Australian or New Zealand origin in a well equipped facility located in Bulimba, Australia. The manufacturing process is a modified Heat Shock method.
The process includes ultrafiltration, micro filtration and freeze-drying.
Trained personnel following Standard Operating Procedures perform all operations. Each batch has a Manufacturing Record, which is reviewed after batch completion.
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前段时间有个同学因为细胞状态不好，培养基里有小黑点，怀疑是支原体污染。于是检测了一下，果真是的，就买了支原体清除剂。别说效果还真好，3天后细胞状态就好了。
既然细胞状态好了，就停用了，没想到一停用，细胞状态就又差了。把培养箱、细胞房都清理一遍还是不行，就问到了我，既然这些因素都排除了，最后只有一个可能了，刚买的Gibco的胎牛血清，本来心里还想，同学实验室真是土豪啊，我就问了价格，南美胎牛1500……澳州胎牛血清3000……，简直惊讶到了，现在Gibco的血清简直是一货难求，澳州胎牛血清已经炒到了8000+了。
就断定这个血清肯定是假的，用检测试剂盒检测了一下，果然是这个问题，假血清真是害人啊，这些无良奸商……由于支原体比较小，滤器过滤不掉，所以大家买血清的时候一定要先试用，检测一下支原体（现在好多大牌子都会有支原体检测报告）
这里来分享一下支原体检测方法：
1，PCR检测法。
2支原体检测跑胶图-20084539882.jpg
这个Primers是依据支原体基因组中高度保守的16SrRNA编码域而设计的，只需简单的PCR反应就可检测M.Arginini,M.Fermentans,M.Hyorhinis,M.Orale,M.Salivarium,M.Hominis,M.Pneumonia等常见的支原体。
2，DNA萤光染色法
利用萤光染剂（bisbenzimide,Hoechst33258）侦测支原体污染。此染剂会结合到DNA之Adenosine-Thymidine(A-T)rich区域，因为支原体之DNA中A-T含量占多数（55～80%），所以可将其染色而侦测。被支原体污染之细胞经染色后，在细胞核外与细胞周围可看到许多大小均一之萤光小点，即为支原体之DNA，证明有支原体之污染。
Cell+细胞卫士__MycoTestKit(支原体检测试剂盒)：
支原体：黑色的，好象多为多形，培养液一般会浑浊，原体感染，国内血清很多都没有做支原体阴性检测，而支原体是牛血清中最常见的微生物之一。而且它不能用过滤的办法除去。支原体感染细胞以后，细胞病变不很明显，只是慢慢死去。
MycoTestKit是利用降落聚合酶链式反应技术（TD-PCR）对支原体16SrRNA基因高度保守区域特异性片段进行扩增检测。该方法灵敏度高，特异性强，可用于各种生物材料（如细胞培养基、实验动物分泌物、动物血清等）支原体感染的检测。
Cell+细胞卫士__NocardTreatment(支原体清除剂)：
（1）推荐NocardTreatment的稀释比例为1:1000，例如：1mL培养基加入1μL的NocardTreatment；
（2）弃去旧的培养基，用PBS将细胞清洗干净，再加入新鲜的含有NocardTreatment的培养基，1天1次，连续处理3-6天。
血清的基本成分是水，水中溶有蛋白质、脂肪、糖、无机盐、维生素等营养成分，也溶有人体代谢产物。血清中有抗体，这是被称作免疫球蛋白的蛋白质。
你说的应该称抗毒血清。
因制作时是将蛇毒、病原菌产的毒等小量多次地注射到免子、马血管内，每日慢慢加大注射量，一定时间后，因该动物体内产生抗体，经检测，达到一定效价后，就可以抽血。血液分离血清后再经提纯，就成了抗毒血清。
如蛇、白喉、破伤风、狂犬病抗毒血清等都是如此制备，用来治疗相应毒素反应。
该生物制剂是异种血清，对人会有过敏反应，注射前均要做皮试。阴性可注射，阳性必需作脱敏疗法才行。
热门血清品牌：
1.Gibco
【特点】
已通过ISO9001注册认证
通过最高水平的USP无菌性测试，并经过三重0.1μm过滤
具有ISIA可追溯性认证，血清的生产符合最高的可追溯性标准
澳洲来源特别适用于要求低风险的研究应用
购买推荐：http://www.biomart.cn/infosupply/27094281.htm
2.Hyclone
购买推荐：http://www.biomart.cn/infosupply/2520990.htm
【特点】
Sciencell的每一批胎牛血清均经过严格的质控和测试，包括近300种人类原代细胞筛选。胎牛血清经无菌过滤，pH值在7左右，内毒素不超过0.625EU/ml，且无病毒成分。
购买推荐：http://www.biomart.cn/infosupply/8505015.htm
国产血清推荐：
1.杭州四季青
购买推荐：http://www.biomart.cn/infosupply/33827963.htm
2.金源康
【特点】
质控标准：采自健康新生牛，两次0.1um过滤，无支原体；产品质量符合《中国药典》2015版标准。
产品特点：低lgG，低内毒素，促细胞生长优良
应用范围：娇贵细胞培养，种细胞的继代等。
推荐购买：
以下产品限时买二送一
新生牛血清（超级）http://www.biomart.cn/infosupply/20221746.htm
胎牛血清（优极）http://www.biomart.cn/infosupply/20323300.htm
胎牛血清（超级）http://www.biomart.cn/infosupply/25018976.htm
3.CellMax
推荐购买：
限时暑期特惠
优级胎牛血清http://www.biomart.cn/infosupply/14975942.htm
标准胎牛血清http://www.biomart.cn/infosupply/14975944.htm
澳洲特级胎牛血清http://www.biomart.cn/infosupply/14975935.htm
谁的答案详细、真实分就给谁...(勿灌水，否则必究之)
本人做动物实验，每组设7只，需要测定小鼠的相关指标，初次接触有几点不明白
1、测定指标时，是否可以每组7只，每只测定一次，一共测定7次？
2、是否可以每组选择3只，每只测定一次，共3次？
3、是否可以每组选择3只，每只自身平行测定3次，共9次？
4、测定指标时，是否可以前3只鼠测定A指标，后4只鼠测定B指标吗？
请问1、2、3中的三个方法哪种方法是否具有可行性？4中的测定方法是否可以？
想做临床的血标本的一些代谢组学研究，不知道应该用血浆还是血清，大家的说法好像也不太一致，请大神指导啊，最好有些参考文献，谢谢！
