IDestroyyeast 1.AspiratemediumandwashcellinPBS. 2.Incubatecellsat37oCfor5mininnon-dilutedantibiotic-antimycotic. 3.Incubatecellsat37oCfor5minintrypsin.Spinthemdown(100g,5min).DONOTEXCEED100g. IISecurecellsfromcrosscontamination 4.Slowlyandcarefullyaspiratesupernatant.ResUSPendcellsin0.5mlofregularmedium. NB!Weassumethatyourregularmediumcontains1xantibiotic. 5Transfercellstoanewtubeanddilutethemwithregularmediumto6ml. IIIPreparecellculturesforregularculturing 6.Seedcellson30mmPetridishes:1:1,1:3,1:7,1:15,1:31,1:63and1:127(cellsuspension/regularmedium). 7.Changethemediumbeforeyouleaveforhome. 8.Nextday:checkforresults