

Anti-GSK3-α, total Antibody
Glycogen Synthase Kinase 3 (GSK3) belongs to the serine/threonine family of protein kinases thatare involved in intermediary metabolism. GSK3 serine kinase is encoded by two related genes called GSK3A (51kD) and GSK3B (46kD). GSK3 plays a role in intracellular signaling pathways. GSK3 is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors. It also plays a role in the WNT and PI3K signaling pathways.
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可以用移液器将细胞吸出来并高速离心,沉淀重悬于PBS中洗涤,接着就可以裂解提取蛋白了。可以用超声,酶解等等,裂解后离心收集上清。
做的比较好的,一般都是上海地区的,你可以看下基尔顿生物。
我刚开始做转染,悬浮细胞,分别做过表达和敲减,看了很多文献,大都没有提及转染后是用转染的这同一批细胞同时做pcr,wb,cck8,凋亡,细胞周期;还是说这次转染只做pcr或wb,再转染一次做cck8或细胞周期。剩下的功能试验均同前,转染一次做一次?我养的是悬浮细胞,转染后做cck8这些功能试验前需要离心换液吗?跪谢解答!
细胞状态与密度;转染试剂的类型、用量;DNA的品质、用量;转染复合物的品质、作用细胞的时间长度。

