Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. CD45 plays an essential role in lymphocyte activation (2).
Isotype: Murine IgG2a kappa
Immunogen: Peripheral blood mononuclear cells
Specificity: Antibody C11 recognizes all isoforms of CD45.
References:
1. U. Dianzani, et al, (1992) Eur J Immunol 22: 365-371.
2. I.S. Trowbridge & M.L. Thomas, (1994) Annu Rev Immunol 12: 85-116.
3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 386-399.
4. D. Leitenberg, et al, (1996) J Exp Med 183: 249-259.
5. M Vigano, de Girolamo, et al. (Feb 2017) J Exp Orthopaedics 4: 8.
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细胞质中有各种细胞器,细胞内的化学反应大多都是在细胞质中进行,因此是代谢中心.
细胞核中有DNA,是遗传信息库,是细胞代谢和遗传的控制中心.
解析:1.细胞质基质(也叫胞质溶胶)是指除细胞器外细胞质的其余部分。细胞质基质是活细胞进行新陈代谢的主要场所。
2.细胞新陈代谢的次要场所是:细胞核、线粒体基质、叶绿体基质等基质。
不破坏细胞结构,同步动态侦测细胞有氧呼吸,糖酵解OCR/ECA(总或乳酸ECA);
i.过氧敏感荧光素(或pH敏感荧光素),Ex340-380/535/Em630-680nm,实时测量线粒体/胞内/胞外重要代谢指标,荧光素为非结构性结合可逆转改变;
ii.可同时或单个测量OCR/ECA或其他参数,不会做成浪费,可采用时间分辨荧光技术可加强系统信噪比
iii.通过两个加药口,可对检测细胞加入适当抑制剂/刺激实时监测细胞对不同毒素作用