| Cytochalasin Cinhibits actin polymerization |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
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- Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
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| Cas No. | 22144-76-9 | SDF | Download SDF |
| Chemical Name | (3S,3aR,6S,6aR,7E,10S,12R,13E,15R,15aR)-15-(acetyloxy)-3,3a,6,6a,9,10,12,15-octahydro-6,12-dihydroxy-4,5,10,12-tetramethyl-3-(phenylmethyl)-1H-cycloundec[d]isoindole-1,11(2H)-dione | ||
| Canonical SMILES | O[C@H]1[C@H]2[C@@]3([C@@H](/C=C/[C@@](C)(O)C([C@@H](C)C/C=C/2)=O)OC(C)=O)C([C@H](CC4=CC=CC=C4)NC3=O)C(C)=C1C | ||
| Formula | C30H37NO6 | M.Wt | 507.6 |
| Solubility | Soluble in ethanol;Soluble in methanol;Soluble in DMSO;Soluble in dimethyl formamide | Storage | Store at -20°C |
| Physical Appearance | A white powder | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Cytochalasin C inhibits actin polymerization.
The cytochalasins are cell-permeable fungal metabolites inhibiting actin polymerization. This interferes with such diverse processes as cell growth, movement, phagocytosis, degranulation, as well as secretion.
In vitro: Previous study described the first results of actin assembly assays in the presence of the different cytochalasins. Acceleration of actin assembly in the presence of several cytochalasins was apparent from the more extensive assembly at shorter times. Cytochalasin C and its analogs, cytochalasin D, H, and J made up the same Group, which was characterized by fast assembly, so that the extent of assembly was reached before the first FPR data trace could be obtained, less than 4 min into the reaction. Thus, the members of this group including cytochalasin C were both strong accelerators of assembly and also inhibitors of steadystate extent of assembly.Moreover, cytochalasin C and D were found to be much stronger inhibitors than cytochalasin H and J, which were moderate and weak inhibitors, respectively. In addition, the effects of these cytochalasins on the diffusion coefficients of actin filaments at steady state was also have examined. Results showd that cytochalasin D and H had significantly higher diffusion coefficients. In contrast, cytochalasin C and cytochalasin A, B, J displayed a weak shortening activity [1].
In vivo: In zebrafish, cytochalasin D at 0.2 μM gave an approximate LD50, while cytochalasin B was fully tolerated at 5 μM, and gave an LD50 of 10 μM. Cytochalasin C was tolerated fully at 1 μM, which was ten-fold higher than the level for cytochalasin D that was tolerated [2].
Clinical trial: Up to now, there is no clinical data reported.
References:[1] Walling, E.A.,Krafft, G.A. and Ware, B.R. Actin assembly activity of cytochalasins and cytochalasin analogs assayed using fluorescence photobleaching recovery. Archives of Biochemistry and Biophysics 264(1), 321-332 (1988).[2] Trendowski M, Wong V, Wellington K, Hatfield S, Fondy TP. Tolerated doses in zebrafish of cytochalasins and jasplakinolide for comparison with tolerated doses in mice in the evaluation of pre-clinical activity of microfilament-directed agents in tumor model systems in vivo. In Vivo. 2014 Nov-Dec;28(6):1021-31.
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可以用CCR3的抗体标记其他细胞，再反推中性粒细胞所占的比例吗？肺泡灌洗液中主要有嗜酸性粒细胞，淋巴细胞，中性粒细胞和巨噬细胞。
有几个疑问
1：荧光标记到细胞是标记到细胞表面还是细胞质内？
2：荧光应该随着细胞的分化和增殖逐渐消失？是不是分化增殖越快，荧光消失速度越快？
3：有哪些容易操作，成本便宜的荧光物质？
谢谢各位战友
比如你用的CD1a－FITC（如果是鼠单抗IgG1，那对照抗体就要用相同物种的非特异性IgG1-FITC）。注意浓度要相同。一般提供抗体的公司BD，santa cruz等有提供的。其他就按照说明书的推荐浓度和孵育时间。
