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Description
Details
Description:Mouse monoclonal antibody to human cluster of differentiation 3 (CD3) antigen
Purification: Prootein G affinity purified
Product Type:Primary antibody
Target Protein:Human peripheral T cell CD3 antigen
Immunogen:Human peripheral T lymphocytes
Fusion Myeloma:Sp2/0-Ag14
Specificity:This antibody recognizes human peripheral blood T cell CD3 antigen.
Reactivity: Human, others not tested
Host / Isotype:Mouse, IgG2a Kappa
Formulation:Lyophilized from a solution in 0.01M PBS pH7.2
Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.
Storage: Store at -20oC
Research Area:Immunology and Oncology
Background:
CD3 is a surface marker expressed on the cell membrane of all matured T-cells and is virtually not found on the surface of other cells. Therefore, CD3 is used as a T-cell specific marker. In addition, CD3 is involved with T-cell activation by functioning as a co-receptor in the T-cell receptor complex (which is comprised of T cell receptor, ζ-chain and CD3).
Applications:Immuno-fluorescence Staining
References:
1. Kebing Wang et al., Dendritic cells transduced with a PSMA-encoding adenovirus and cocultured with autologous cytokine-induced lymphocytes induce a specific and strong immune response against prostate cancer cells. Urologic Oncology: Seminars and Original Investigations 27 (2009) 26–32.
2. Jun Pang et al., Enhanced antitumor effects by the coculture of allotumor RNA-pulsed dendritic cells with autologous cytokine-induced killer cells on hormone-refractory prostate cancer. Cancer Investigation, 25:527–534, 2007.
3. Jian Wang et al., Inducible Costimulator (ICOS) Enhances the Cytolytic Activity of Cytokine-Induced Killer Cells Against Gallbladder Cancer In Vitro and In Vivo. Cancer Investigation, 27:244–250, 2009.
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
Product Specificity | mAb anti-Human CD3, JXT3 |
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Application | IF |
Size | 0.1 mg |
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(2)荧光标记法 : 使用二乙酸荧光素(FDP)、碘化丙啶(PI)或异硫氰酸荧光素钠标记的荧光染料与细胞共孵育,用流式细胞仪检测荧光染色阳性细胞的比率。此法其实是(1)法的“荧光”版,但其在灵敏性和准确性方面明显要优于后者。
(3)硝酸镧(La)示踪法: 在正常的生物组织中镧微粒可沉积于细胞间隙,但不能穿过具有1~ 2nm 微小间隙的细胞膜性结构(包括细胞膜和细胞器膜),也不能穿过细胞间的紧密连接。在膜性结构通透性增高时, 镧微粒则可进入细胞、细胞器和紧密连接内, 并在电镜下显示, 镧盐标记技术被认为是一种有效的监测细胞膜通透性变化的标记技术。
(4)LDH释放法: 在正常情况下,细胞内大分子物质LDH 是不能通过细胞膜的, 但在细胞膜受损伤而通透性增加时,可通过受损的细胞膜释放出来。LDH 能较好地反映细胞膜损伤程度。类似的还有检测细胞外K+的漏出率等。
有几个疑问
1:荧光标记到细胞是标记到细胞表面还是细胞质内?
2:荧光应该随着细胞的分化和增殖逐渐消失?是不是分化增殖越快,荧光消失速度越快?
3:有哪些容易操作,成本便宜的荧光物质?
谢谢各位战友
比如你用的CD1a-FITC(如果是鼠单抗IgG1,那对照抗体就要用相同物种的非特异性IgG1-FITC)。注意浓度要相同。一般提供抗体的公司BD,santa cruz等有提供的。其他就按照说明书的推荐浓度和孵育时间。