
Uniprot ID : | P0DTC2 |
Alternative Name : | nCov, Sars-Cov-2 |
Format : | Purified |
Amount : | 100 µg |
Isotype : | Rabbit IgG |
Immunogen Information : | A partial length recombinant coronavirus Spike Protein (amino acids 850-1050) was used as the immunogen for this antibody. |
The spike (S) protein of nCoV/SARS-CoV-2/COVID-19 is one of the structural glycoproteins that remains embedded in viral envelope and acts as the fundamental component of early viral infection of nCoV/SARS-CoV-2/COVID-19 upon binding the host receptor. The nCoV/SARS-CoV-2/COVID-19 has a trimeric spike protein which has two main domains such as S1 domain for receptor binding and S2 domain for membrane fusion and several specific cleavage sites in S1- S2 boundary junction that needs a novel, endocytic protease- primed cleavage to get activated during infection. It mainly binds to the furin protein on the cell membrane which performs trypsin like proteolytic cleavage and then the protein gets activated facilitating its entry into the host. This transmembrane spike protein of nCoV/SARS-CoV-2/COVID-19 shares binding property to the Angiotensin Converting Enzyme 2 (ACE2) likely to that of SARS- CoV. The high affinity of nCoV/SARS-CoV-2/COVID-19 Spike protein for human ACE2 may contribute to the apparent ease with which nCoV/SARS-CoV-2/COVID-19 can spread from human-to-human and make nCoV/SARS-CoV-2/COVID-19 pandemic.
Purification : | Protein A Purification |
Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Recommended dilutions: WB: 2-4 µg/ml. However, this need to be optimized based on the research applications.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
1. Minimal system for assembly of SARS-CoV-2 virus like particles: Heather Swann, Abhimanyu Sharma, Benjamin Preece, Abby Peterson, Crystal Eldridge, David M. Belnap, Michael Vershinin, Saveez Saffarian bioRxiv 2020.06.01.128058; doi: https://doi.org/10.1101/2020.06.01.128058
There are currently no product reviews Write a review on this product! |
Customers who purchased this product also purchased
Recombinant Anti-SARS-CoV-2 Sp...


Recombinant Anti-SARS-CoV-2 Sp...


Monoclonal Antibody to ZIKA NS...


Monoclonal Antibody to ZIKA En...


Monoclonal Antibody to ZIKA E ...


Monoclonal Antibody to Ebola G...


Monoclonal Antibody to Ebola G...


Coronavirus (COVID-19) Spike A...


Mouse Monoclonal Antibody to M...


Mouse Monoclonal Antibody to p...


Mouse Monoclonal Antibody to G...


Mouse Monoclonal Antibody to S...


Mouse Monoclonal Antibody to A...


Mouse Monoclonal Antibody to I...

Recombinant Anti-SARS-CoV-2 Sp...


Recombinant Anti-SARS-CoV-2 Sp...


Monoclonal Antibody to ZIKA NS...


Monoclonal Antibody to ZIKA En...


Monoclonal Antibody to ZIKA E ...


Monoclonal Antibody to Ebola G...


Monoclonal Antibody to Ebola G...


Coronavirus (COVID-19) Spike A...


Mouse Monoclonal Antibody to M...


Mouse Monoclonal Antibody to p...


Mouse Monoclonal Antibody to G...


Mouse Monoclonal Antibody to S...


Mouse Monoclonal Antibody to A...


Mouse Monoclonal Antibody to I...

Most viewed Products

Recombinant Human Thioredoxin-Like ...


Monoclonal antibody to Human PD-L1 ...


Monoclonal Antibody to Caspase-3 (P...


Polyclonal Antibody to Beta actin


Monoclonal Antibody to GAPDH (Clone...


Monoclonal Antibody to Human IL-1be...


Monoclonal antibody to B7-H4 (Clone...


Polyclonal Antibody to Beta Tubulin


Peroxidase conjugated Goat anti Mou...


Monoclonal Antibody to EpCAM (CD326...

ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
1 mmol/LEDTA(pH 8.0)
因为含有以上两种物质,所以称为TE。
配制分三步:
1)1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
2)0.5 M EDTA(pH 8.0)50 ml的配制:称取EDTA-Na2·2H2O 9.306 g,加超纯水35 ml,剧烈搅拌,用约1 g NaOH颗粒调pH至8.0,定容至50 ml。(EDTA二钠盐需加入NaOH将pH调至接近8.0时,才会溶解。)
3)1×TE(10 mM Tris-HCl,pH 8.0;1 mM EDTA,pH 8.0)的配制:
作用:
TE缓冲液是弱碱性,对DNA的碱基有保护性,(DNA在它是的稳定性较好,不易破坏其完整性或产生开环及断裂),包括提取好的DNA也要放在TE缓冲液是保存. 10mMTris-Hcl,pH有7.47.68.0三种。
EDTA调到8.0是为了更好溶解,其他只要调到相应pH就可以。Tris在7-8附近缓冲能力很强,所以加8.0的EDTA下去后,不会改变pH。
TBST中含有Tris-Hcl,NaCl,Tween20这三种物质,是做WESTERNBLOT中常用的一种缓冲液。
TBST缓冲液的配制
1000ml×TBST的配置
先称量NaCl40g,倒入烧杯中,加DDW蒸馏水400ml,再称量NaCl47.6g,倒入刚才的那个烧杯中(PS:由于NaCl的量太多,一次称量不方便,所以分两次称量,且易于溶解)。往烧杯中加入Tris—HCl缓冲液100ml,最后加(吐温20)5ml,转入1000ml容量瓶中,在定容,转移即可。
TBST缓冲液的应用:
1.主要用于免疫组化和原位杂交,酶联免疫等实验中,清洗免疫印。
2.迹膜;
注意事项:
1.TBST缓冲液,PH7.2-7.5;
2.颜色为无色透明液体;
3.为了您的安全和健康,请穿实验服并戴防护手套操作;
1、组成成分:
A、1×TE缓冲液:10mmol/LTris.Cl;1mmol/LEDTA,pH8.0。
B、1×TAE缓冲液:40mmol/LTris-乙酸;2mmol/LEDTA,pH8.0。
C、1×TBE缓冲液:45mmol/LTris-硼酸;1mmol/LEDTA,pH8.0。
2、用途:
A、TE缓冲液:一般用作溶解剂或保持剂,常用于溶解DNA,能稳定储存DNA。
B、TAE缓冲液:生物学中使用最广泛的核酸电泳缓冲液,主要用于DNA的琼脂糖凝胶电泳。
C、TBE缓冲液:生物学中常用的核酸电泳缓冲液,主要用于DNA的琼脂糖凝胶电泳。
3、TAE和TBE缓冲液的选择:
TAE和TBE缓冲液都可以用于DNA的琼脂糖凝胶电泳,两者各有利弊,应根据实际情况选择不同的缓冲液。
4、注意:
TBE浓溶液长时间存放后会形成沉淀物,出现沉淀后应予以废弃。

