Recombinant Human Tau 412 (1N4R) Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
SP-501
| Formulation | 2.1 mg/ml (50μM) in PBS pH 7.4 |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Tau
Tau is a microtubule-associated protein expressed primarily in neurons. Carboxy-terminal domains of Tau associate with and stabilize microtubule structure, while other domains bind to the plasma membrane. Abnormal Tau phosphorylationmay result in the self-assembly of tangles of paired helical and/or straight filaments, which are involved in the pathogenesis of Alzheimer"s disease and other neurodegenerative diseases. Properly folded Tau is highly soluble, but when the protein becomes misfolded it forms insoluble aggregates that can damage cytoplasmic functions, interfere with axonal transport and ultimately lead to cell death. There are multiple forms of Tau--this412 amino acid isoform is known as "1N4R," "Isoform Tau-E"or "Tau 412" and is referenced in UniProt as P10636-7. This recombinant protein is untagged.
- Billingsley M.L. & Kincaid R.L.(1997) Biochem. J. 323:577
- Bloom G.S. (2014) JAMA Neurol. 71: 505
- Cripps D. et al.(2006) J. Biol. Chem. 281: 10825
- Harada A. et al (1994) Nature 369: 488
- Lei P. et al. (2010) Int. J. Biochem. Cell Biol. 42: 1775
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问：1如才能提高柱子吸附蛋白量？
2样品中的核酸对吸附有无太大影响？
3我拟采用阴离子交换树脂，问pH8.0条件下除去核酸可否？
4怎么提高样品的澄清度？
另外还想问一下，还有其他可行的破碎方法吗
谢谢
我怀疑溶菌酶有问题，因为其消化的30min内菌液几乎没大变化，而我曾看到别人用溶菌酶消化时菌液变的挺粘稠。
今下午再做时还是没什么变化，但发现溶菌酶在冰上静置2－3h后竟然沉淀下来了，而且上清和下面的白色溶菌酶分界明显，是我的溶菌酶配的有问题吗？出现这种情况溶菌酶还能用吗？我是－20度保存的。
下一步怎么处理菌液呢？
请高手多多指教！
大家请赐教！
