Toensurereliableexperimentalresultsandenablethehighestperformancefromyoursolutions,itisessentialtousetherightfilter.Thefeaturesofafiltersuchasdiameter,poresize,membranematerial,andhousing,areallimportant.Atossila,wehaveselectedarangeofsyringefilterswhichcanbeusedformostapplicationsandareallcompatIBLewithoursolvent-safepolypropylenesyringes. |
|
|
|
| |
ProductList
Non-AqueousSyringeFilters
| ProductCode | C101 | C102 | C103 | C104 | C2006S2 | C2006S1 | ||
| PoreSize | 0.1μm | 0.22μm | 0.45μm | 1.2μm | 0.1μm | 0.45μm | ||
| Membrane | PTFE(Hydrophobic) | PVDF(Hydrophobic) | ||||||
| FilterDiameter | 13mm | |||||||
| FiltrationArea | >1.3cm2 | |||||||
| RetainedFluid | <100μl | |||||||
| PackSize | 100filters | |||||||
| PackageType | Lowdustpolyethylenebox | |||||||
*Ifyourequireaporesizeordiameternotlistedinthetablepleasecontactustoseeifwecanprovideyouwithasyringefiltertomeetyourspecifications.
AqueousSyringeFilters
| ProductCode | C2007S1 | C2007S2 | C2008S1 | C2008S2 | C2009S1 | C2009S2 | ||
| PoreSize | 0.45μm | 0.1μm | 0.45μm | 0.1μm | 0.45μm | 0.1μm | ||
| Membrane | Nylon | PTFE(Hydrophillic) | PES | |||||
| FilterDiameter | 13mm | |||||||
| FiltrationArea | >1.3cm2 | |||||||
| RetainedFluid | <100μl | |||||||
| PackSize | 100filters | |||||||
| PackageType | Lowdustpolyethylenebox | |||||||
*Ifyourequireaporesizeordiameternotlistedinthetablepleasecontactustoseeifwecanprovideyouwithasyringefiltertomeetyourspecifications.
Specifications
| FilterHousing | Polypropylene/HighDensityPolyethylene |
| InletAttachment | FemaleLuerlock |
| OutletAttachment | MaleLuerSlip |
| MaximumOperatingTemperature | 60℃ |
| Sterilization | Autoclaveat121℃for20minutes |
MembraneFiltrationOverview
Membranefiltrationisamethodofremovingparticulatesfromsolutionwithouttheneedforanyheating.Thisfiltrationmethodistypicallyusedinotherprocesses,suchassublimationanddistillation.Itworkssimplybyrestrictingthemovementofparticlesaboveaspecificsizethroughaporousmaterial.Typicallymembranefiltrationisseparatedintoseveralcategoriesdependentuponparticlesize.Thesecategoriesare:particlefiltration,microfiltration,ultrafiltration,nanofiltraion,andreverseosmosis.Thesedifferentcategoriescoverparticlesizes,rangingfromcentimetresdowntoangstroms.Belowisanimagehighlightingtherangecoveredbymicrofiltrationmembraneshighlightingthetypesofmaterialsthatcanbefilteredout.
Membranefiltrationisusedinavarietyofscientificfieldsincludingbiotechnology,pharmaceuticals,medicine,chemistry,andsemiconductormanufacturing.Eachfieldhasverydifferentrequirementsforthefiltersandthechoiceoffiltertypecandependuponthesolventbeingfiltered,thesizeofparticlesthatneedremoving,whetherthefiltersneedtobesterile,thevolumeofsolutionbeingfiltered,andthetemperatureofyoursolution.Ifyouareunsureaboutwhatparticularmembranewouldbestsuityourneedspleaseseethefollowingsection.
ChoosingtheCorrectSyringeFilter
AtOssila,weofferfivedifferentmembranematerialsforoursyringefilters:
- HydrophobicPTFE(Polytetrafluoroethylene)
- HydrophilicPTFE
- HydrophobicPVDF(Polyvinylidenefluoride)
- PES(Polyethersulfone)
- Nylon
PTFEcanbeusedwithalmostanysolventduetoitslowreactivityandlowextractables.Additionally,PTFEhasextremelyhighthermalstABIlity,allowingforawiderrangeofprocessingtemperaturescomparedtostandardmembranematerials.However,PTFEisnaturallyhydrophobic,whichcanmakethefilteringofaqueoussolutionsdifficult.Tocounterthis,weoffertheoptionofhydrophilicPTFE-inwhichthemembranesurfacehasbeentreatedtoimprovethewettingonaqueoussolutions.
PVDFlikePTFEisnaturallyhydrophobicandlikePTFEoffershighchemicalresistanceagainstawiderangeofdifferentchemicals.PVDFalsohastheadvantageoflowproteinbindingincomparisontomaterialssuchasPTFE.
Duetoitsnaturallyhydrophilicnature,PESiscompatiblewithaqueoussolutionsandalcohols.Infact,PESishighlyrecommendedfortheuseofforfilteringaqueoussolutions.Thisisduetothereducedpresenceofextractablesfromnothavingtotreatthemembranessurfacewithwettingagents.Inaddition,duetoitsextremelylowproteinbinding,PESofferssuperiorperformanceforusewithBIOLOGicalsamplesandculturemedia.
LikePES,nylonisnaturallyhydrophilic -makingitsuitableforuseforfilteringaqueoussolutions,andlikePESitresultsinalowlevelofextractableswithinthefilteredsolution.NylonhasahigherchemicalresistancethanPESmakingitsuitableforawiderrangeoforganicsolventsincludingketones,esters,DMFandDMSO.
Belowisatableshowingthesizesofsolutionssuitablefordifferentfilterdiameters:
| Diameter | SolutionVolume |
| 5mm | <1ml |
| 13mm | <10ml |
| 25mm | <50ml |
Wehavefoundthat13mmdiameterfiltersprovidethebestcombinationoffilteringcapability/capacity,withminimalsolutionloss.
Theporesizeofyourchosenfilterdependsonthematerialyouwishtoremove.Asthemembranematerialhasaporesizedistributionofaroundanaveragesize,itispossiblethatparticlesbiggerthanthestatedporesizemaypassthrough.Werecommendchoosingaporesizejustbelowtheminimumsizeofparticleyouwishtoremove.
Tothebestofourknowledgethetechnicalinformationprovidedhereisaccurate.However,Ossilaassumenoliabilityfortheaccuracyofthisinformation.Thevaluesprovidedherearetypicalatthetimeofmanufactureandmayvaryovertimeandfrombatchtobatch.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
它最初由在德国生物学家罗伯特·科赫手下工作的细菌学家朱利斯·理查德·佩特里（1852－1921）于1887年设计，故又称为“佩特里皿”。
====================
其实就是常见的圆形的上下盖培养皿，你可以看看它的英文，就叫做petri dish，直译过来就叫做——佩特里小碟子——也就是皮氏培养皿
=====================
第二，三段为原创手打……第一段为摘抄
如题~
已经基本养好了贴壁的原代细胞了，准备鉴定了，看到好多都在说细胞爬片的问题，我可以不做爬片直接在皿里操作吗？具体需要注意些什么呢？感谢各位大神
ps：大皿和6孔板里细胞密度、培养条件等等都几乎一样。
我看园子里有人提过类似的问题，但还没人给出答案，希望有经验或有想法的战友帮忙分析分析啊~谢谢！
想问下各位大神，本人用皿养的PC-9GR细胞突然有大片飘起，也并没有成团状飘起，求解决方法？
所谓上皿式指的是称量盘在支架上面；而称量盘吊挂在支架下面的为下皿式。
目前使用较为广泛的是上皿式电子天平。
实验需要请问有没有养THP-1细胞的同仁？能否寄一皿我，我在广州南方医科大学，不胜感激！
同学说传代必须是一皿传多皿，一皿传一皿就不算传代~求高手指点~~
传代后5天，依然没有长满，怕影响活力，想传代和冻存，不知这样是否可以？？
