- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody to human C reactive protein (CRP)
Purification:Protein G affinity purified
Product Type:Primary antibody- Capture antibody in matched antibody pair
Target Protein:Human CRP
Immunogen:Purified CRP from human pleural fluid
Fusion Myeloma:Sp2/0-Ag14
Specificity:No detectable cross-reaction with human normal serum proteins.
Species Reacitvity: Human, others not tested
Host / Isotype: Mouse, IgG1 Kappa
Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.
Storage: Store at -20oC
Research Area:Inflammatory and cardiovascular diseases
Background:
C –reactive protein (CRP) is an acute phase protein that is comprised of five identical 23kDa subunits. The protein increases rapidly in response to inflammation, trauma and infection, and drops to normal level when the conditions no longer present. CRP plays a role in innate immunity by binding to phosphachlolin on the surface of damaged cells or certain microbes, and activating the C1q complex in complement system. CRP is primarily produced by hepatocytes under the stimulation by inflammatory cytokines including IL-6 and TNF-alpha. Elevated CRP level is implicated with bacterial infection, autoimmune diseases, trauma and post-surgical complications. Study has shown that people with higher CRP baseline have a higher disposition to develop cardiovascular diseases such as heart attack and stroke.
Application:
ELISA:This antibody can be used as capturer in ELISA for CRP detection in combination with HRP conjugated CRP polyclonal antibody [Cat No: AI-20009P].
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-human CRP, 63F4, Capture |
|---|---|
| Application | ELISA |
| Size | 0.1 mg |
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想通过亲和层析来纯化抗血小板特异性抗体（抗GPIIb/IIIa抗体和抗GPIbα抗体），能否做到呢？谢谢！
一抗的英语是 resistance No.1，二抗resistance No.2.
我的实验是这样的，先纯化质粒，然后做酶切，再纯化DNA。
最后一步其实也可以用切胶回收试剂盒，我怕做不好，所以想选用DNA纯化试剂盒，但不知跟质粒DNA提取试剂盒是否有区别，因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊，觉得那个是提取质粒的小提啊。所以产生这个疑问，请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗？
用哪个公司的DNA纯化试剂盒比较好。谢谢啦！
