Inidentifyingpeptidesfromproteinswithaknownsequence,itisoftenusefultobeabletopredicthowapeptidewillmigrateduringelectrophoresis,chromatography,ortwo-dimensionalpeptidemapping. ThePepsortprogram,whichrunsasanadd-ontotheGCG/UWpackageofsequenceanalysisprograms,willcalculateelectrophoreticandchromatographicmobilitiesforpeptidesderivedfromasequencefile.ThisisaprogramwrittenbyattheSalkInstitutebyLisaCaballerousingdataofTonyHunter"s.Itisbynomeansfoolproof,butcanpredictthemobilitiesofsomepeptidesveryaccurately. TorunPepsort,youneedtohavetheGCGsequenceanalysispackageonyourcomputer. YoucanobtainPepsortfromtheSalkInstitutebyftpwithyourbrowserusingthislink ftp://axp1.salk.edu/mobility/ Thisdirectorycontainsareadmefilethatexplainswhattodownloadandhowtoruntheprogram. Partsofthereadmecanbefoundonthislinkedpage. PepsortisusedinconjunctionwithaprogramcalledMobilitythatwillplotthepredictedmobilitiesofyourpeptides.Itisincludedinthesameftpdirectoryonaxp1.salk.edu.
