- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Type III collagen is the second most abundant collagen in tissues and is found most commonly in tissues exhibiting elastic properties such as skin, lungs, intestinal walls and walls of blood vessels. It is a homotrimer comprised of three alpha-1 chains and resembles other fibrillar collagens in structure and function. It is synthesized as procollagen, similary to collagen I, but the N-terminal propeptide remains attached in the mature fibrillar type III form.
Symbols/Related Terms:
- COL1A1
- COL1A2
- COL3A1
- Collagen type III, alpha 1
- osteogenesis imperfecta
Data/Specifications
Purity:
Canine collagen type I and III - 98%
Canine Collagen (other types) - <1%
Non-collagen proteins - below 0.5%
Form: 10 mg lyophilized, salt-free
Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.
Source: Canine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III were purified by using differential salt precipitation.
Reconstitution: Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils.
Storage:Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Literature/Support
Canine Type I and III Collagen Insert (PDF)
Note: inserts are for review only. Please refer to the insert shipped with your product to ensure the most up-to-date revision is being used.
How To Use
Key Applicatons:
- Coating material for cell culture studies.
Note: May not be suitable for 3-D gel formation.
Related Products
ebiomall.com
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1.如果在引物的5’引物引入了T7启动子，3‘引物也引入了polyT,PCR扩增出全长片段，回收纯化之后可以用来直接做体外转录么？为什么看到文献里都是先把这个PCR片段连接到载体里面，扩增之后提取出来线性化之后，再进行体外转录。是不是转录对模板的量有要求？
2.如果使用一些带有T7启动子序列的载体比如pBluescriptSK，在启动子序列和酶切位点之间还有多余的一段序列，这段序列也会被启动子转录，这一小段多余的序列是否会对接下来的转染以及病毒拯救带来影响？
3.有人在做反向遗传么，哪些载体可以用来进行体外转录？插入片段为7.5K。
建议：做肝功能检查再结合肝功能情况遵医嘱进行抗病毒治疗
2) 克隆技术使用使倾向于量繁殖现种群利用价值体,按自规律促进整种群优胜劣汰.意义说,克隆技术干扰自进化程.
3) 克隆技术种昂贵技术,需要量金钱物专业士参与,失败率非高.莉277实验唯.虽现发展更先进技术,功率能达2-3%.
4) 转基物提高疾病传染风险.例,产药物牛奶牛染病毒,种病毒能通牛奶染病
5) 克隆技术应用于体导致代遗传性状工控制.克隆技术引起争论核能否允许发育初期类胚胎进行遗传操作.伦理家所能接受.
6) 克隆技术用创造超,或拥健壮体格却智力低.且,克隆技术能够类效运用,男性失遗传意义.
7) 克隆技术家庭关系带影响巨.由父亲DNA克隆孩看作父亲双胞胎兄弟,延迟几十已.难设想,发现自另外完全复制品,（或）受
（1）种相互作用否细胞内自发即蛋白细胞命否同间表达且定位同区域
（2）某些蛋白依赖于遍蛋白蛋白酶解途径员具普遍蛋白间相互作用能力
（3）些实际没任何相互作用相同模体（motif）两亲a-螺旋蛋白质间发相互作用十酵母双杂交技术直消除假阳性面断改进并且已取较效〔23〕
酵母双杂交应用遇假阴性现象所谓假阴性即两蛋白本应发相互作用报告基表达或表达程度甚低至于检测〔4〕造假阴性原主要两 面：融合蛋白表达细胞毒性应该选择敏性低菌株或拷贝数低载体二蛋白间相互作用较弱应选择高敏菌株及拷贝载体目前假阴性现象虽实验主要问题应予重视
内容发布在蚂蚁淘和螺旋网上，有兴趣的战友也可以去那里看一下。
内容分三部分：
一、概述
1、克隆性染色体异常是肿瘤的特征
2、染色体异常常见的类型
3、染色体异常的检测方法
二、荧光原位杂交及其探针
1、荧光原位杂交的原理
2、荧光原位杂交的探针
三、荧光原位杂交探针的制备和荧光原位杂交（按试验流程介绍）
:D
FISH.pdf(1237.91k)
