
Name | 101Bio Denaturing Protein Solubilization Reagent | ||||||
Cat. # | P5W9 How to pay with ![]() Also can buy from : ![]() ![]() | ||||||
Application | This reagent is formulated for dissolving protein pellets extracted using protein kits (such as 101Bio Cat.#: P503, P511, P513, P518 and P523 kits). This product is for research use only. | ||||||
Product Size | 10 ml | ||||||
Description | This reagent is formulated for dissolving protein pellets extracted using protein kits (such as 101Bio Cat.#: P503, P511, P513, P518 and P523 kits).The solubilized proteins are denatured due to the presence of an anionic detergent (SDS).Proteins dissolved in this reagent is mainly used for SDS-PAGE electrophoresis and Western blotting.In some cases, the solubilized protein can also be used for immunoprecipitation and trypsin digestion if SDS is removed using our SDS-Remover(Cat. # P5W12). | ||||||
Shipping / Storage | Ship at ambient temperature and stored at RT. | ||||||
Shelf Life | 12 months | ||||||
Manual (protocol) | ![]() | ||||||
Components |
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Name | Cat. # | Size | ||
---|---|---|---|---|
101Bio HRP Substrate Kit | P5W1 | 30 ml /30 ml | ||
101Bio Antibody Enhancer | P5W2 | 30 ml | ||
101Bio WB Stripping Solution | P5W3 | 250 ml | ||
101Bio Ponceau S Stain | P5W4 | 250 ml | ||
101Bio Peroxidase Suppressor | P5W5 | 30 ml | ||
101Bio High Efficiency Protein Precipitation kit | P5W6 | 30 ml /30 ml | ||
101Bio Red Blood Cell Lysis Buffer | P5W7 | 250 ml | ||
101Bio WB Blocking Solution | P5W8 | 250 ml | ||
101Bio Denaturing Protein Solubilization Reagent | P5W9 | 10 ml | ||
101Bio Non-Denatured Protein Solubilization Reagent | P5W10 | 10 ml | ||
101Bio Protein Solubilization Reagent for MS | P5W11 | 2 ml | ||
101Bio SDS-Remover | P5W12 | 10 ml | ||
101Bio Albumin Depletion Reagent for Plasma and Serum | P5W13 | 10 ml | ||
101Bio Protein A+G Agarose Beads | W0349 | 1 ml | ||
101Bio Protease Inhibitor Cocktail (100x) | W2200 | 1 ml | ||
101Bio Yeast Total Protein Extraction Kit for SDS-PAGE | Y203-50 | 50 rxn |

![]() | Exosome Isolation | Purity > 95% |
![]() | Protein Extraction | 1 min total protein, 40 min membrane protein |
![]() | 3D Cell Culture Gel | 30% < mkt=""> |
![]() | PCR Kits | 50% < mkt=""> |
![]() | Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput |
![]() | Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |
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第一代热启动Taq酶是一种DNA聚合酶。 第一代指的是直接从Taq(这是一种细菌)体内提取的天然聚合酶,没有进行化学修饰的,为了达到更好的聚合催化效果,往往需要经过酶工程处理,得到的聚合酶催化效率更高。 热启动是指这种酶的反应温度条件
Taq DNA polymerase是从克隆有Thermu aquaticus DNA Polymerase基因的大肠杆菌经诱导表达后,再经过柱纯化分离出来的一个约94 KD的重组蛋白。无外源核酸酶和细菌DNA污染,稳定性好,特异性强,适用于常规PCR扩增。
https://www.nature.com/articles/ni.3830.epdf?referrer_access_token=-Pjl2rt1nZv_1Z8JIoznz9RgN0jAjWel9jnR3ZoTv0PyprITrhfI0J5ucCRfVO-aui0xL-EVR7N2JR4xNKbXmYtj4yXphjh43zAiP6r70OSxNUbkTgT9CKdP6rhb181-RAaL3nGfbbFavHe89R525v6eyOP-tI8-8kcwVAVmKJT1UwT0dPSmOENdZt03DCnok55kvZGMawTEwNU1ehYjDg%3D%3D&tracking_referrer=news.sciencenet.cn
针对DDX家族成员在RNA识别和代谢及其在调控抗病毒天然免疫应答中发挥的重要功能,通过筛选多种DDX家族成员在病毒感染巨噬细胞天然免疫应答中的作用,发现了DDX46能显著抑制病毒感染诱导的干扰素表达
DDX46能结合到抗病毒效应分子mRNA的CCGGUU保守基序上,当病毒感染时DDX46与m6A去甲基化酶ALKBH5结合增加,使得与DDX46结合的抗病毒效应分子mRNA发生去甲基化修饰而导致其核滞留,阻滞了这些抗病毒效应分子的蛋白表达从而降低干扰素产生,最终抑制了抗病毒天然免疫应答反应
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
核酶(ribozyme)是指一类具有催化功能的RNA分子,通过催化靶位点RNA链中磷酸二酯键的断裂,特异性地剪切底物RNA分子,从而阻断基因的表达.
核酶的催化功能与其空间结构有密切关系,目前已知有多种特殊结构的核酶:
RNaseP的RNA碱基(M1 RNA)、锤头型、发夹型丁型肝炎病毒RNA、1类内含子和2类内含子,大多有hammerhead structure。
不同的核酶可分为两类:
1 剪切型核酶:只剪不接,如M1 RNA
2 剪接型核酶:该酶具有序列特异的内切核酸酶、RNA连接酶等多种酶活性,如1类和2类内含子向左转|向右转

