Name | 101Bio Denaturing Protein Solubilization Reagent | ||||||
Cat. # | P5W9 How to pay with Also can buy from : | ||||||
Application | This reagent is formulated for dissolving protein pellets extracted using protein kits (such as 101Bio Cat.#: P503, P511, P513, P518 and P523 kits). This product is for research use only. | ||||||
Product Size | 10 ml | ||||||
Description | This reagent is formulated for dissolving protein pellets extracted using protein kits (such as 101Bio Cat.#: P503, P511, P513, P518 and P523 kits).The solubilized proteins are denatured due to the presence of an anionic detergent (SDS).Proteins dissolved in this reagent is mainly used for SDS-PAGE electrophoresis and Western blotting.In some cases, the solubilized protein can also be used for immunoprecipitation and trypsin digestion if SDS is removed using our SDS-Remover(Cat. # P5W12). | ||||||
Shipping / Storage | Ship at ambient temperature and stored at RT. | ||||||
Shelf Life | 12 months | ||||||
Manual (protocol) | 101Bio.com Denaturing Protein Solubilization Reagent | ||||||
Components |
|
Name | Cat. # | Size | ||
---|---|---|---|---|
101Bio HRP Substrate Kit | P5W1 | 30 ml /30 ml | ||
101Bio Antibody Enhancer | P5W2 | 30 ml | ||
101Bio WB Stripping Solution | P5W3 | 250 ml | ||
101Bio Ponceau S Stain | P5W4 | 250 ml | ||
101Bio Peroxidase Suppressor | P5W5 | 30 ml | ||
101Bio High Efficiency Protein Precipitation kit | P5W6 | 30 ml /30 ml | ||
101Bio Red Blood Cell Lysis Buffer | P5W7 | 250 ml | ||
101Bio WB Blocking Solution | P5W8 | 250 ml | ||
101Bio Denaturing Protein Solubilization Reagent | P5W9 | 10 ml | ||
101Bio Non-Denatured Protein Solubilization Reagent | P5W10 | 10 ml | ||
101Bio Protein Solubilization Reagent for MS | P5W11 | 2 ml | ||
101Bio SDS-Remover | P5W12 | 10 ml | ||
101Bio Albumin Depletion Reagent for Plasma and Serum | P5W13 | 10 ml | ||
101Bio Protein A+G Agarose Beads | W0349 | 1 ml | ||
101Bio Protease Inhibitor Cocktail (100x) | W2200 | 1 ml | ||
101Bio Yeast Total Protein Extraction Kit for SDS-PAGE | Y203-50 | 50 rxn |
Exosome Isolation | Purity > 95% | |
Protein Extraction | 1 min total protein, 40 min membrane protein | |
3D Cell Culture Gel | 30% < mkt=""> | |
PCR Kits | 50% < mkt=""> | |
Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput | |
Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |
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可分为以下几个类群:(1)依赖DNA的DNA聚合酶;(2)依赖RNA的DNA聚合酶;(3)依赖DNA的RNA聚合酶;(4)依赖RNA的RNA聚合酶。前两者是DNA聚合酶,它使DNA复制链按模板顺序延长。如在原核生物中仅就大肠杆菌中已被发现的就有三种(分别简称为PolⅠ,PolⅡ和PolⅢ等);DNA聚合酶只能在有引物的基础上,即在DNA或RNA引物的3′-OH延伸,这DNA的合成方向记为5′→3′。换言之DNA聚合酶催化反应除底物(αNTP)外,还需要Mg2+ 、模板DNA和引物,迄今细胞内尚无发现可从单体起始DNA的合成。同样,上述(3)和(4)是催化RNA生物合成反应中最主要的RNA合成酶,它们以四种三磷酸核糖核苷(NTP)为底物,并需有DNA模板以及Mn2 及Mg2 的存在下,在前一个核苷酸3′-OH与下一个核苷酸的5′-P聚合形成3′,5′-磷酸二酯键,其新生链的方向也是5′→3′。RNA聚合酶也大量存在于原核和真核生物的细胞中。如大肠杆菌RNA聚合酶分子量4.8×105,由5条多肽链组成,分别命名为α,α,β,β′,和γ,全酶可用α2ββ′λ表示。真核生物RNA聚合酶分子大于5×105,由10~12个大小不等亚基组成。聚合酶除作为自然界生命活动中不可缺少的组分外,在实验室中大多用作生命科学研究的工具酶类之一。
只要是细胞,就得走向衰老,现代研究表明,细胞的衰老和端粒的变短有关系。端粒就是DNA,随着细胞分裂次数的增多,端粒在不断变短,端粒酶就是组织端粒变短的。然而正常细胞中,端粒酶的活性受抑制,端粒酶是怎么染端粒变长的呢?实际上端粒酶是由RNA组成的,可以根据碱基互补配对逆转形成DNA,使得变短的端粒变长。所以说端粒酶可以逆转录形成端粒,使端粒不减短!
1970科家致癌RNA病毒发现种特殊RNA病毒聚合酶,该酶能RNA模板,根据碱基互补原则.程与般病毒转录向相反,故称逆转录,催化程酶称逆转录酶,发现哺乳物胚胎细胞裂淋巴细胞含.
逆转录病毒称携带逆转录酶病毒,先侵入宿主细胞病毒RNA模板,靠酶形DNA环化,合宿主细胞染色体原病毒形式宿主细胞代代传.
HIV(艾滋病病毒)典型逆转录病毒
TERT是端粒酶逆转录酶
人的端粒酶是由端粒酶逆转录酶、端粒酶RNA和一种假尿嘧啶合成酶组成
不过。。。。我时常用那种过期一两年的。。。。也能反转出来。只要你保存得当就可以了。一般是-20以下保存。
有DNase、RNase、核酸酶S1等,可水解相应的DNA和RNA,核酸酶S1可降解单链DNA和RNA,用量增大也可降解双链核酸。它可用于切去ds-cDNA合成中产生的发夹环。
(2)修饰酶
有些酶可在其他酶的作用下,将酶的结构进行共价修饰,使该酶活性发生改变,
核酶的催化功能与其空间结构有密切关系。
不同的核酶可分为两类:
1 剪切型核酶:只剪不接,如M1 RNA。
2 剪接型核酶:该酶具有序列特异的内切核酸酶、RNA连接酶等多种酶活性。
加工场所在生物体细胞质(或相关实验设备中)。