Trisisafairlycomplex,Tris-bufferedphysiologicalsalinesolution.ItisusedtowashcellsandispreferabletoTD,unlessyouneedaCa++/Mg++freesolution. Itis: 136.8mMNaCl5mMKCl0.9mMCaCl20.7mMMgCl2(6H20)0.7mMNa2HPO425mMTris-HCl,pH7.4 TDisavariantofTristhatlacksthecalciumandmagnesium.Itisusedtowashcellspriortotrypsinization.Calciumandmagnesiuminhibitcelldispersal.Tryp/TDis0.25%BactotrypsininTD.Itisstoredfrozen.PBSisaphosphate-bufferedphysiologicalsalinesolution.ItisusefulifyouwanttoavoidTrisbuffer.LikeTris,itcontainscalciumandmagnesium.ThereisararelyusedvariantlackingcalciumandmagnesiumcalledPBSa. PBSis: 136.8mMNaCl2.5mMKCl0.8mMNa2HPO41.47mMKH2PO40.9mMCaCl20.5mMMgCl2(6H2O) VEisTDthatcontainsEDTA.OneveryoldbrandnameforEDTAisVersene,hencetheacronym.VEishelpfulintrypsinizingveryadherentcells.NotethatEDTAinhibitsthegrowthofsomecells,soitmustbegottenridofinmanycases.Bart"sCookbook--
ThekitchenmakesTris,TD,Tryp/TD,PBS,andVE.
