10xsaltscanbeautoclavedandstored.Turnsyellowafterautoclaving.MEMFAFix 10xMEMFASalts 1part10xMEMFAsalts 1MMOPS 1part37%formaldehyde 20mMEGTA 8partswater 10mMMgSO4
ForlacZstaining:fixfor30min-1houratroomtemp
Forinsitus:fixfor2hours.
Bouin"sFix | ||
70%picricacid | ||
25%37%formaldehyde | ||
5%glacialaceticacid |
Bouin"sfixstopsthechromogenicsubstratesveryeffectivelyandalsohelpsremovesomeofthetrappedbackgroundstain.ItstopsthecolorreactionveryeffectivelyandmuchbetterthanMEMFAwhichcanresultintheembryosturningblackeverywhere.Bouin"sdoesstaintheembryosyellowhowever.ThisyellowstaincanberemovedwithmultiplewashesinbufferedethanolorPBS-Tween.DonotuseBouin"stofixbeforeinsitu.WedidthisonceaccidentlyandwereunabletodetectRNAatall.