- Plumbagin
- 360A
- 360A iodide
- AT13387
- Vorinostat (SAHA, MK0683)
- R8-T198wt
| PyridostatinDrug used for promoting growth arrest |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
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- Purity = 98.00%
- COA (Certificate Of Analysis)
- HPLC (Retest)
- MS
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
Related Biological Data
Related Biological Data
| Cell experiment [1]: | |
Cell lines | HeLa, HT1080, U2OS and WI-38 cell lines |
Preparation method | The solubility of this compound in DMSO is >20.85 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 0–40 μM for 72 h |
Applications | A previous study investigated the growth inhibition after 3 days of exposure to pyridostatin on a panel of four human cell lines: HeLa (adenocarcinoma), HT1080 (fibrosarcoma), U2OS (osteosarcoma), and WI-38 (normal lung fibroblasts), the latter being non-cancerous. Pyridostatin showed growth inhibition at high nanomolar to low micromolar concentrations against these tested cell lines. In addition, pyridostatin exhibited an 18.5-fold selectivity for HT1080 cells over WI-38 cells. |
References: [1] Müller S, Sanders D A, Di Antonio M, et al.Pyridostatin analogues promote telomere dysfunction and long-term growth inhibition in human cancer cells. Organic & biomolecular chemistry, 2012, 10(32): 6537-6546. | |
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| Cas No. | 1085412-37-8 | SDF | Download SDF |
| Synonyms | RR-82;RR82;RR 82 | ||
| Chemical Name | 4-(2-aminoethoxy)-2-N,6-N-bis[4-(2-aminoethoxy)quinolin-2-yl]pyridine-2,6-dicarboxamide | ||
| Canonical SMILES | C1=CC=C2C(=C1)C(=CC(=N2)NC(=O)C3=CC(=CC(=N3)C(=O)NC4=NC5=CC=CC=C5C(=C4)OCCN)OCCN)OCCN | ||
| Formula | C31H32N8O5 | M.Wt | 596.64 |
| Solubility | ≥20.85 mg/mL in DMSO, ≥30.87 mg/mL in EtOH with gentle warming, ≥9.66 mg/mL in H2O with ultrasonic and warming | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Pyridostatin is a synthetic small-molecule stabilizer of G-quadruplexe [1].G-quadruplexe is a kind of secondary structure of DNA that usually exists in the end of the chromosome or the telomeres. Since G-quadruplexe is also enriched in the promoters of a serious of proto-oncogenes including c-kit, K-ras and Bcl-2, they are thought to participate in the regulation of gene replication and transcription. Besides that, G-quadruplexe has been found to affect the elongation, replication and capping of telomeres. Based on these findings, a lot of small molecules that can interact with G-quadruplexe have been designed and synthesized to help demonstrate the existence and roles of G-quadruplexe or to be developed as selective anti-cancer drugs. It has been reported that some small molecules interacting with G-quadruplexe can cause the progressive shortening of telomeres and subsequently the active the DNA damage response resulting in cell cycle arrest. Among these molecules, pyridostatin is a synthetic small-molecule stabilizer of G-quadruplexe with the ability to adapt the dynamic and diverse structures of G-quadruplex. Pyridostatin competed for binding with the telomere associated proteins and induced the dysfunction of telomeres [1 and 2]. In the FRET melting assay using human telomeric G-quadruplex-forming sequence and ds-DNA, pyridostatin showed maximal stabilization effect of the G-quadruplex sequence at concentration of 1 μM while showed no effect on the ds-DNA. In a panel of three cancer cell lines (HeLa, U2OS and HT1080) and a normal cell line (WI-38), treatment of pyridostatin significantly inhibited cell growth with IC50 values of 0.89 to 10 μM after 72 hours. The selectivity of pyridostatin against HT1080 cells was 18-fold higher than that against the normal cells [1 and 3]. References:[1] Mela I, Kranaster R, Henderson R M, et al. Demonstration of ligand decoration, and ligand-induced perturbation, of G-quadruplexes in a plasmid using atomic force microscopy. Biochemistry, 2012, 51(2): 578-585.[2] Müller S, Sanders D A, Di Antonio M, et al. Pyridostatin analogues promote telomere dysfunction and long-term growth inhibition in human cancer cells. Organic & biomolecular chemistry, 2012, 10(32): 6537-6546.[3] McLuckie K I E, Di Antonio M, Zecchini H, et al. G-quadruplex DNA as a molecular target for induced synthetic lethality in cancer cells. Journal of the American Chemical Society, 2013, 135(26): 9640-9643.
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tRNA不是一条直的单链，而是弯曲的，呈现一个三叶草的形状。在弯曲的部位，tRNA自己的碱基跟自己的碱基互补配对连起来，碱基对中存在氢键。
其他的RNA一般为单链不存在氢链，但双链的由于碱基互补配对存在氢键。
探针就是DNA弹针。用于检测特定的DNA片段。
基因探针，即核酸探针，是一段带有检测标记，且顺序已知的，与目的基因互补的核酸序列(DNA或RNA)。基因探针通过分子杂交与目的基因结合，产生杂交信号，能从浩翰的基因组中把目的基因显示出来。根据杂交原理，作为探针的核酸序列至少必须具备以下两个条件：①应是单链，若为双链，必须先行变性处理。②应带有容易被检测的标记。它可以包括整个基因，也可以仅仅是基因的一部分；可以是DNA本身，也可以是由之转录而来的RNA。
进行分子突变需要大量的探针拷贝，后者一般是通过分子克隆（molecular cloning）获得的。克隆是指用无性繁殖方法获得同一个体、细胞或分子的大量复制品。当制备基因组DNA探针进，应先制备基因组文库，即把基因组DNA打断，或用限制性酶作不完全水解，得到许多大小不等的随机片段，将这些片段体外重组到运载体（噬菌体、质粒等）中去，再将后者转染适当的宿主细胞如大肠肝菌，这时在固体培养基上可以得到许多携带有不同DNA片段的克隆噬菌斑，通过原位杂交，从中可筛出含有目的基因片段的克隆，然后通过细胞扩增，制备出大量的探针。
为了制备cDNA 探针，首先需分离纯化相应mRNA，这从含有大量mRNA的组织、细胞中比较容易做到，如从造血细胞中制备α或β珠蛋白mRNA。有了mRNA作模板后，在逆转录酶的作用下，就可以合成与之互补的DNA(即cDNA)，cDNA与待测基因的编码区有完全相同的碱基顺序，但内含子已在加工过程中切除。
寡核苷酸探针是人工合成的，与已知基因DNA互补的，长度可从十几到几十个核苷酸的片段。如仅知蛋白质的氨基酸顺序量，也可以按氨基酸的密码推导出核苷酸序列，并用化学方法合成。
二：主要功能：①运输功能②在逆转录作用中作为合成互补链DNA链的引物。③在细菌细胞壁、叶绿素、脂多糖和氨酰磷脂酰甘油的合成中都与某些tRNA的参与有关。
tRNA的二级结构：单链内某些区域靠氢键配对形成局部双链，并折叠形成其二级结构-三叶草型结构
三级结构是在二级结构基础上进一步折叠形成，呈“倒L”型。
的问题在于使siRNA导入细胞，
将sirna导入细胞内常见的方法是
将靶向特定基因的大约21碱基长短的双链 siRNAs small interfering RNAs。
或者是45 50 mer的发夹结构 RNA 转染到细胞。
此外通过质粒表达siRNAs 同样可以抑制特定基因的表达。
各种系统都可以使用，AlleleID7.0没搞的定
本软件只作为学习研究之用，研究完后马上删除，支持正版。。。
感谢ddd198599及其他司机的信息及启发
http://www.stemcell8.cn/thread-20673-1-1.html
AlleleID6破解版.rar(44952.85k)
我是这样进行预测的：在“SearchMode:”中选择“rRNAscanonly:”，在“sorce”中选择“mito/chloroplast”,粘贴序列，运行程序。得到了21个tRNA，而鱼中应该是22个tRNA，我觉得很奇怪，在“SearchMode:”和“rRNAscanonly:”中进行其它的设置，都不能得到22个tRNA.
我看的一篇文献“CompletemitochondrialDNAsequenceoftheJapanese
flyingfishCypselurushiraii”中也是说22个tRNA,但是我把文章提交的序列（Genbank号为AB182653.）拿到网上提交，只有21个tRNA，我觉得很奇怪。
不知道是怎么回事？请高手指点，那个软件好像是比较权威的。
