
Intended Use:
The AESQC quality controls are reagents obtained from pools of human sera available with different autoantibodies that allow a multi parametric control in autoimmune disease diagnosis.These controls were developed to help lab managers to ensure that analytical error stays within acceptable limits.
AESQCs are unassayed ready to use reagents which should be handled in the same way as patient samples and can be used for monitoring the performance of ELISA and FARR assays.
Our AESQC controls are formulated to give positive reactions for the respective autoantibody and the lab should establish their own working ranges for each new lot depending from the test system and procedures being used.
Antibodies:
hRF IgM isotype, CCP IgG isotype, LCP igG isotype (Linear citrullinated peptides)
ebiomall.com






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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!
各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!
便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合

