ProductHighlights:
TheNexteraMatePairLibraryPreparationKitisanidealapproachfordenovosequencing,genomefinishing,andthedetectionofstructuralvariation.ItiscompatIBLewithlargeDNAgenomes—eventhemostcomplexgenomessuchasthosederivedfromcancer.
- SequenceprecioussampleswhenlimitedDNAisavailable
- Generatehighlydiverselibrarieswithpremierdataquality
- Preparesequencing-readylibrariesinlessthantwodays
Requires10TimesLessDNAInputThanOtherProtocols
Bothgel-freeandgel-plusprotocolsareavailable:
- Thegel-freeprotocolallowsmatepairsequencingwhenlimitedDNAisavailable.Itisdesignedforapplicationssuchasdenovoassemblyofsmallgenomes.
- Thegel-plusprotocolisidealforchallengingmatepairapplications,suchasdenovoassemblyofcomplexgenomes.
IncreasedLibraryDiversityandDataQuality
Thekitprovidesidentifiablejunctionsequencesforaccurateidentificationofthetwohalvesofthematepairfragment.
- OnlybiotinylatesDNAmoleculesatfragmentationsites,avoidingtroublesomeinternalbiotinylation
- Allowsforthecreationofmillionsofuniquefragments
- Increasedlibrarydiversitygeneratesfewerduplicatereadsandpremierdataquality
FastandSimpleMatePairWorkflow
TheNexteraMatePairkitusesTruSeqDNALibraryPreparationmaster-mixedreagents,therebyreducingthenumberofassaystepsandhands-ontimerequired.
- Fewerpipettingstepssimplifiesworkflowandreducessampleloss
- Gel-freeprotocoloptionandon-beadreactionssimplifypurificationstepsandshortenprotocolhands-ontime
Specifications:
AssayTime | 1.5-2days |
Hands-OnTime | 1.5-2hours |
InputQuantity | 1ugDNAforgel-freeprotocol,4ugDNAforgel-plusprotocol |
Multiplexing | Canmultiplexupto12samples,dependingongenomesize.CompatiblewithmostlargeDNAgenomes. |
MechanismofAction | Methodsthattargetthejunctionsiteoflargecircularizedfragments.Mechanicalfragmentation(COVARIS). |
SystemCompatibility | MiSeq,HiSeq3000,HiSeq2500,HiSeq4000 |
SpeciesCategory | Other,Mammalian,Mouse,Human,Rat,Plant,Bacteria |
VariantClass | SingleNucleotidePolymorphisms(SNPs),StructuralVariants,Insertions-Deletions(indels) |
Method | Whole-GenomeSequencing,DeNovoSequencing,Long-ReadSequencing |
Technology | Sequencing |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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