
ProductHighlights:
TheNexteraMatePairLibraryPreparationKitisanidealapproachfordenovosequencing,genomefinishing,andthedetectionofstructuralvariation.ItiscompatIBLewithlargeDNAgenomes—eventhemostcomplexgenomessuchasthosederivedfromcancer.
- SequenceprecioussampleswhenlimitedDNAisavailable
- Generatehighlydiverselibrarieswithpremierdataquality
- Preparesequencing-readylibrariesinlessthantwodays
Requires10TimesLessDNAInputThanOtherProtocols
Bothgel-freeandgel-plusprotocolsareavailable:
- Thegel-freeprotocolallowsmatepairsequencingwhenlimitedDNAisavailable.Itisdesignedforapplicationssuchasdenovoassemblyofsmallgenomes.
- Thegel-plusprotocolisidealforchallengingmatepairapplications,suchasdenovoassemblyofcomplexgenomes.
IncreasedLibraryDiversityandDataQuality
Thekitprovidesidentifiablejunctionsequencesforaccurateidentificationofthetwohalvesofthematepairfragment.
- OnlybiotinylatesDNAmoleculesatfragmentationsites,avoidingtroublesomeinternalbiotinylation
- Allowsforthecreationofmillionsofuniquefragments
- Increasedlibrarydiversitygeneratesfewerduplicatereadsandpremierdataquality
FastandSimpleMatePairWorkflow
TheNexteraMatePairkitusesTruSeqDNALibraryPreparationmaster-mixedreagents,therebyreducingthenumberofassaystepsandhands-ontimerequired.
- Fewerpipettingstepssimplifiesworkflowandreducessampleloss
- Gel-freeprotocoloptionandon-beadreactionssimplifypurificationstepsandshortenprotocolhands-ontime
Specifications:
AssayTime | 1.5-2days |
Hands-OnTime | 1.5-2hours |
InputQuantity | 1ugDNAforgel-freeprotocol,4ugDNAforgel-plusprotocol |
Multiplexing | Canmultiplexupto12samples,dependingongenomesize.CompatiblewithmostlargeDNAgenomes. |
MechanismofAction | Methodsthattargetthejunctionsiteoflargecircularizedfragments.Mechanicalfragmentation(COVARIS). |
SystemCompatibility | MiSeq,HiSeq3000,HiSeq2500,HiSeq4000 |
SpeciesCategory | Other,Mammalian,Mouse,Human,Rat,Plant,Bacteria |
VariantClass | SingleNucleotidePolymorphisms(SNPs),StructuralVariants,Insertions-Deletions(indels) |
Method | Whole-GenomeSequencing,DeNovoSequencing,Long-ReadSequencing |
Technology | Sequencing |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!


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