
ProductHighlights:
FastLibraryPrep,OptimizedforSmallGenomes,PCRAmplicons,andPlasmids
- Rapidlibrarypreparation–completelibraryprepinaslittleas90minuteswithonly15minutesofhands-ontime
- Fasttimetoresults–gofromDNAtodatain8hourswithourbenchtopsequencers.
- Optimizedforsmallgenomes,PCRamplicons,andplasmids–onelibraryprepkitformanyapplications
- Innovativesamplenormalization–eliminatestheneedforlibraryquantificationbeforesamplepoolingandsequencing
WithNexteratechnology,DNAissimultaneouslyfragmentedandtaggedwithsequencingadaptersinasingletubeenzymaticreaction.NexteraXTsupportsultra-lowDNAinputofonly1ng.Itenablesawiderangeofinputsamples,including smallgenomes,PCRampliconsgreaterthan300bp,plasmids,microbialgenomes,concatenatedamplicons,anddouble-strandedCDNA.
Multiplexingofupto384samplesperNexteraXTlibraryisalsoavailableforthoseprojectsrequiringgreaterthroughput.Plus,thekitincludesaninnovativebead-basedsamplenormalizationthateliminatestheneedforlibraryquantificationpriortopoolingandsequencing.LibrariespreparedwithNexteraXTkitsarecompatIBLewithallIlluminasequencers.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
Specifications:
AssayTime | 90minutes |
Hands-OnTime | 15minutes |
MechanismofAction | EnzymaticFragmentation |
Multiplexing | Upto384uniquelyindexedsamplesmaybepooledandsequencedtogether. |
InputQuantity | 1ngDNA |
SpeciesCategory | Drosophila,AnySpecies,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria |
SystemCompatibility | MiSeq,NextSeq550,NextSeq500,MiSeqFGxinResearchMode |
Method | ShotgunSequencing,Whole-GenomeSequencing,DeNovoSequencing,16SrRNASequencing,AmpliconSequencing,TargetedDNASequencing |
VariantClass | SingleNucleotidePolymorphisms(SNPs),StructuralVariants |
SpecializedSampleTypes | LowInput,SingleCells |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
NexteraXTLibraryPreparationWorkflowimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-1.png:
ebiomall.com






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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!


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