ProductHighlights:
TruSeqCustomAmpliconisafullycustomizable,amplicon-basedassayfortargetedresequencing.Theassayenablesyoutofocusinterrogationonkeyregionsofgenomicinterest.Upto1536ampliconscanbesequencedinasinglereactionusingasimpleworkflow.Thishighlytargetedapproachoffersunparalleledefficiencyfordiscovering,validating,andscreeninggeneticvariantsinareasofresearchfocus.
Requiringaslittleas50ngofinputgenomicDNA(gDNA),thiskitcanbeusedwithawiderangeofsampletypes.TheABIlitytomultiplexupto1536ampliconsperreactionallowscoverageofupto650kbofcumulativesequence.Further,anintegrateddualindexingschemesupportsupto96samplesperrun,enablingyoutofullybenefitfromdesktopsequenceroutputcapabilities.
ThisassayprovidesimprovedperformanceindifficulttoaddressGC-richregions,aswellasincreasedlibraryyield,uniformity,andstability.Additionally,optimizedamplificationstepsenablesampleinputrequirementstodropfrom150ngto50ng.
SimplifiedProbeDesign
DesigntargetedTruSeqCustomAmpliconoligonucleotideprobeswithDesignStudio,afree,easy-to-useonlinetoolthatprovidesoptimizedcoverage.
LearnmoreaboutDesignStudio
StartaprojectinDesignStudionow(loginrequired)
SimpleDataAnalysis
AutomateddataanalysisusingtheBaseSpaceTruSeqAmpliconAppallowsresearcherstoeasilyreviewprojectdata,includingvariantsdetected,acrossmultipleruns.
SpeciesCompatibility
TruSeqCustomAmpliconsupportshuman,mouse,rat,andbovinesamples.DesignStudiodesignalgorithmsarealsoavailableformaize,rice,pig,dog,soybean,chicken,andsheep;however,assayperformancecannotbeguaranteedastheresultingassayshavenotbeentested.Inaddition,IlluminaConciergeservicescandesigncustomassaysand/orprovideassayoptimizationsupportforanyspeciesofinterest.
InquireaboutIlluminaConciergeservicesFindanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 1day |
Hands-OnTime | 4hours |
InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
Multiplexing | Upto384samplespersequencingrun |
MechanismofAction | Amplification |
VariantClass | ShortTandemRepeats(STRs),SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels) |
SystemCompatibility | HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq1500,NextSeq500,MiSeqFGxinResearchMode,HiSeq2500,HiSeq4000 |
SpeciesCategory | Other,Bovine,Mouse,Human,Rat |
SpecializedSampleTypes | FFPE |
Technology | Sequencing |
AutomationCapability | LiquidHandlingRobots |
Method | AmpliconSequencing,CustomSequencing |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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