
ProductHighlights:
GeneratemRNA-focusedsequencinglibrariesfromtotalRNA,withenhancedmultiplexcapABIlityandasimplifiedworkflowwithmaster-mixedreagents.
Kitsfeature24uniqueindexes,deliveringenhancedmultiplexperformanceforprocessinglargenumbersofsamples(includingupto384RNA-SeqsamplesonasingleHiSeq2500Systemrun).
Master-mixedreagentseliminatethemajorityofpipettingstepsandreducetheamountofclean-up,ascomparedtopreviousmethods,minimizinghands-ontime.Thisresultsineconomical,high-throughputRNAsequencingstudiesachievedwiththeeasiest-to-usesamplepreparationworkflowofferedbyanyNGSplatform.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | ~10.5hours |
Hands-OnTime | ~4.5hours |
InputQuantity | 0.1-1ugtotalRNAor10-400ngpreviouslyisolatedmRNA(fromspecieswithpolyAtails) |
ContentSpecifications | Capturesthecodingtranscriptome(withoutstrandinformation) |
MechanismofAction | Oligo-dTbeadscapturepolyAtails |
Multiplexing | Upto24-plexperlane |
SystemCompatibility | GenomeAnalyzerIIx,HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |
SpeciesCategory | Other,Mammalian,Bovine,Mouse,Human,Rat |
VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,NovelTranscripts,TranscriptVariants |
Technology | Sequencing |
Method | mRNASequencing |
AutomationCapability | LiquidHandlingRobots |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!


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