General Category:
- Monoclonal antibodies » Biodefense
Disease Category:
- Biodefense » Monoclonal Antibodies
MAb FB11 recognizes LPS of virulent and vaccine strains of Fr. tularensis. There is no crossreactivity with Fr. novicida, Br. abortus, Br. suis, Br. melitensis, Br. ovis, Y. pestis, Y. enterocolitica, Y. pseudotuberculosis, E. coli, and V. cholerae.
The binding site for MAb FB11 is located on the O-antigen polysaccharide chain which consists of tetrasaccharide fragments and has the following structure: -4)α-D-GalpNAcAN-(1-4)-α-DGalpNAcAN-(1-3)-β-D-QuipNAc-(1-2)-β-Quip4NFm-(1.
Tetrasaccharide D-GalpNAcAN-(1-4)-α-D-GalpNAcAN-(1-3)-β-D-QuipNAc-(1-2)-β-D-Quip4NFm and trisaccharide D-GalpNAcAN-(1-3)-β-D-QuipNAc-(1-2)-β-D-Quip4NFm compete in ELISA for binding MAb FB11 with LPS of Fr. tularensis.
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量水用精细般瓶装乙醚即
采用6孔板接毒后,铺上低溶解度琼脂糖,第二天就出来了这些病变,正常细胞没有出现这种现象。
按说空斑应该出现多个,但我的六孔板中一个空最多也就出现了2个空斑,也不像文献报道的那样规则,而且很大!
由于没有做梯度稀释,会不会是接毒量过大造成的啊?请大侠指点,万分感谢!

