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The PromoCell Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. The M2-Macrophage Generation Medium DXF contains M-CSF and allows for the generation of M2-polarized macrophages. PromoCells M2-Macrophage Generation Medium DXF is a ready-to-use medium including cytokines for the directed differentiation of M2-like polarized MDM.
The Macrophage Media DXF are defined and xeno-free formulations for use with freshly isolated cells. Due to the utilization of exclusively synthetic, recombinant or plant-sourced materials, this medium is free of all animal-derived components and substances of human origin with human serum albumin as the only exception.
All media are optimized for primary human cells. However, we have received feedback from customers that this medium can also be used for murine macrophages.
To view a list of references where this medium was used with other species/cell types please click here.
Components:
- Basal Medium M2 (250ml)
- SupplementMix M2 (for 250ml)
- CytokineMix M2: M-CSF (for 250ml)


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量水用精细般瓶装乙醚即
采用6孔板接毒后,铺上低溶解度琼脂糖,第二天就出来了这些病变,正常细胞没有出现这种现象。
按说空斑应该出现多个,但我的六孔板中一个空最多也就出现了2个空斑,也不像文献报道的那样规则,而且很大!
由于没有做梯度稀释,会不会是接毒量过大造成的啊?请大侠指点,万分感谢!

