Certificate of Analysis | |
Antibody Clone: | 6A1-IgE |
Vial: | Orange Cap; 200µl |
Origin: | Monoclonal antibody derived from a patient allergic to cat. |
Specificity: | Fel d 1, Felis domesticus allergen. |
Allergen: | Natural Fel d 1 (product code NA-FD1-1) |
IgE Concentration: | 50,000 IU/mL based on ImmunoCAP using WHO International Standard Immunoglobulin E (IgE), human serum (NIBSC code 11/234) |
Quantity: | 10,000 IU in 0.2mL (1.0 IU= ~2.4ng IgE) |
| Formulation: | In phophate buffered saline, pH 7.4 and 0.05% Tween-20. 0.22µm filtered,preservative free. |
Storage: | Maintain at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles. Store product undiluted. No stability data available. |
| Product Resources: | E-6A1 Certificate of Analysis |
| References: | Wurth, M.A. et al., Human IgE mAbs define variability in commercialAspergillus extract allergen composition, JCI Insight. 2018;3(20):e123387. Bazaral, M. and Hamburger, R.N., Standardization and stability ofimmunoglobulin E, J Allergy Clin Immunol 1972; 49:189-191. PMID4622102. |
For research and in vitro diagnostic use only:Not for human in vivo or therapeutic use. | |
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般基克隆物体直接取基包括面几步骤
提取植物或者物基组DNA(试剂盒)————做PCR需要基扩增(顺利2搞定)——扩增基做酶切——基连接想插入载体面(顺利1-2)——鉴定克隆基
步骤都商品化试剂或者试剂盒 要思路清晰做起快 1-2周能完
没证实情况任何没依据推断都假想空想
现在小弟碰见一个难题,我想克隆一个基因,但是我在NCBI一检索,发现别人只发表了这个基因的DNA全长,但是,CDNA没有发表,也没有发表文章,想请问下,在不知道CDNA的情况下,请问我应该如何做这个基因的克隆???(全基因组也不知道,其他生物没有这种基因)
详见:http://zhidao.baidu.com/question/1831844001436785260.html?fr=iks&word=%B2%A1%B6%BE%B5%C4%B8%B4%D6%C6%D6%DC%C6%DA%B5%C4%B9%FD%B3%CC%3F&ie=gbk

