- Overview
- Data/Specifications
- Literature/Support
- How It Works
- Related Products
Overview
Human HtrA1 protease was initially identified in human fibroblasts and belongs to the high temperature requirement factor A (HtrA) family of serine proteases that can be distinguished from other serine proteases by sequence homology, by the presence of a trypsin-type protease domain and one or two carboxyterminal PDZ domains and by their oligomeric architecture.
The loss of mammalian HtrA activity is correlated with severe diseases, including arthritis, cancer, familial ischemic cerebral small vessel disease and age related macular degeneration, as well as Parkinson’s disease and Alzheimer’s disease.
Epigenetic silencing occurs in various cancers, and the loss of HtrA1 correlates with decreased sensitivity to anticancer drugs and increased cell migration. In addition, overexpression of HtrA1 inhibited proliferation in vitro and tumor growth in vivo. These data suggest that HtrA1 might function as a tumor suppressor.
In the extracellular matrix, HtrA1 cleaves numerous secreted proteins, such as fibronectin, decorin, fibro- modulin, aggrecan, type II collagen, biglycan, clusterin, a disintegrin and metalloproteinase domain-contain- ing 9 (ADAM9), vitronectin, α-2-macroglobulin and the amyloid precursor protein fragment Aβ. The degra- dation of extracellular matrix components and the strong upregulation of HtrA1 in samples from patients implicate HtrA1 in arthritic diseases, in which it might affect the degradation of cartilage as well as inflam- mation.
It is unknown, how the cellular distribution of HtrA1 is regulated. In addition, little is known about the inter- action partners of mammalian HtrAs. It will be important to identify proteins that function as determinants of the cellular localization, substrate specificity and regulation of HtrA proteases.
Data/Specifications
Species: human
Sample Type: serum, tissue, cell culture supernates
Sample Size: 500 uL
Standard Curve Range: 0.391 - 25 ng/mL
Sensitivity: 391 pg/ml
Assay Length:4 hours
Literature/Support
Product Insert:
human HtrA1 ELISA Insert (PDF)
Articles/Troublshooting:
ELISA Troubleshooting Guide
ELISA Data Reduction Guide
How It Works
This quantitative assay is based on a two site sandwich format. A highly specific monoclonal antibody against HtrA1 is immobilised on the plate. HtrA1 will be bound to the wells, other components of the sample are removed by discarding/drying by taping and washing of the plate. The analyte is detected in two steps using a secondary biotin-labeled monoclonal antibody and a highly polymerised streptavidin-peroxidase conjugate. Any excess is removed by discarding/drying by taping and washing after each detection step. The amount of peroxidase bound to each well is determined by the addition of TMB Substrate. The reaction is stopped by adding the Stop Solution and the resultant color is read in a microplate reader at 450 nm. The concentration of HtrA1 in a sample is determined by interpolation from the standard curve.
Related Products
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斜率的计算是和电极测量你所配制的溶液的电位有关系的。
三个标准缓冲液，标定的时候一般选择两个就行，如果三个都参与标定，斜率会相应的有差别。
电极的话，分立的电极是参比和测量各一支，分别接在仪器后面，使用时一起测量。复合电极，有的是参比和测量复合电极，应该只有一个球泡。还有一种三复合电极，是参比，测量，还有温度都集于一支电极上，上面除了玻璃球泡，那个小黑的是测量溶液温度的。
请问BAC蛋白浓度测定波长是562，我们这边酶标仪的滤光片波长最接近的是595，这样也可以测吗
酶标仪选择重复性好的，线性范围宽的，故障率底的，售后服务跟的上的厂家的仪器就好了。
1.两种方法哪一种更加准确？
2.那一种方法更方便？
3.需要的仪器上海哪家单位有？
