
- SynonymNGAL,LCN2,Lipocalin-2,24p3,MSFI
- SourceHuman Lipocalin-2, His Tag (LC2-H5222) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Gly 198 (Accession # NP_005555.2).Predicted N-terminus: Gln 21Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.4 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehaloseis added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human Lipocalin-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundLipocalin-2 (LCN2) is also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), MSFI, The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor. LCN2 is strongly upregulated during inflammation and is upregulated by interleukin 1 (but not TNF alpha) in humans. There are indications that some forms of acne could be caused due to the gene not being transcribed, and that Isotretinoin corrects this. NFAT3 (NFATc4)NFAT by blocking the expression of LCN2 inhibits breast carcinoma cell motility. Recent studies have revealed that NGAL plays an important role in the physiopathology of chronic myeloid leukaemia (CML) mediated by BCR-ABL.
- References
- (1)Flo TH, et al., 2004, Nature 432 (7019): 917–21.
- (2)Schmidt-Ott KM, et al., 2007, J. Am. Soc. Nephrol. 18 (2): 407–13.
- (3)Nelson AM, et al., 2008, J. Clin. Invest. 118 (4): 1468–78.
- (4)Fougère, M, et al., 2010, Oncogene 29 (15): 2292–301.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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变阻法计数在大多数细胞计数器中是利用小孔管换能器装置实现的。
在仪器的取样杯内装有一根吸样管,吸样管下部开有一个小孔(宝石制作),因此也叫做小孔管。小孔管内外各置一只铂金电极,两电极间施加一个恒定的电流。测试时,先将待测血液用洁净的电解液充分稀释,使血细胞在电解液中成为游散状态,然后在小孔管上端施以负压,在负压的抽吸下,混有血细胞的电解液便被均匀地抽进小孔管。当血细胞通过小孔时,排开了等体积的电解液,使电解液的等效电阻瞬间变大,这个变大的电阻在恒流源的作用下引起一个等比例增大的电压。当细胞离开小孔附近后,电解液的等效阻值又恢复正常,直到下一个细胞到达小孔。这样血细胞连续地通过小孔,就在电极两端产生一连串电压脉冲。脉冲的个数与通过小孔的细胞个数相当,脉冲的幅度与细胞体积成正比。
首先,电子细胞技术仪是机器计数,全自动计数,快速准确,比人工有效率多了,有条件可以购买一台计数器咯~~bodboge是我们在用的机器
其次,细胞计数板需要人工操作,取样什么的都要注意,不然就会有偏差,而且速度还很慢
所以,总的来说,肯定是机器计数会比较准确
这样的回答可能没分,好和不好都是比较出来的,一群国产的放在一起比总有个性能比较均衡,比较突出性价比好的,
还是没有和说那个厂家的好桂林的呢好像是优利特这个厂家在做,特康的前身呢就是百特,现在的百特呢基本上是在做试剂,这些东西呢。
也搜索的到的,不过答案还是比较接近的,但想知道的是国产那个厂家生产的比较好,而只回答了一半就和介绍了下国产的血球分析仪有那个几个大的厂家,没有和区分那个最好那个第二那个次点,或许一堆鸡蛋里实在挑不出骨头。
具所知呢迈瑞的好点特康实力没有迈瑞好,迈瑞吸引了一批外资在搞研发,桂林的呢好像有个优利特现在出血分析仪,他做的尿分析仪市场的占有还可以,血的反应不是很强烈。
实在是想找个内行的给比较下。

