| Source | M.W. | 4963.55 | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH | ||||
| Formulation | Lyophilized in a solution of acetonitrile and TFA. | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized peptide shouldbe reconstituted in sterile PBS. | ||||
| Stability | The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1 month at 4 °C, but shouldbe stored in aliquots at -80 °C for longer term. Avoid repeated freeze andthaw. | ||||
| Purity | Greater than 95% as determined by RP-HPLC analysis | ||||
| Biological Activity | |||||
| Country of Origin | USA | ||||
Thymosinsare small polypeptides originally isolated from the thymus, but most areactually present in many other tissues. The archetypical β-Thymosin isThymosin-β4, which is a cellular constituent in many tissues. In addition toits role as a major actin-sequestering molecule, Thymosin-β4 has a role intissue repair. Such effect have been shown in animal models of tissue damageand now in human clinical trials. Recently,Thymosin-β4 was shown to stimulate regeneration of adult cardiac progenitorcells in a paper published in Nature (Smart,et al., Nature, 2011, June 8th). AminoAcid Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH
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需要测量转染之后细胞某mRNA表达变化。
实验组给予转染,空白组做对照。
做PCR的时候肯定是要求两组的细胞计数是一样的,请问是转染之前还是转染之后对两组进行计数呢?如果样品两边计数不一样,怎么让做实验的时候两边细胞数目变成一样呢?
型 号:IC1000
品 牌:Countstar
单 价:45000.00元/台
最小起订量:1 台
供货总量:5 台
发货期限:自买家付款之日起 7 天内发货
这样的回答可能没分,好和不好都是比较出来的,一群国产的放在一起比总有个性能比较均衡,比较突出性价比好的,
还是没有和说那个厂家的好桂林的呢好像是优利特这个厂家在做,特康的前身呢就是百特,现在的百特呢基本上是在做试剂,这些东西呢。
也搜索的到的,不过答案还是比较接近的,但想知道的是国产那个厂家生产的比较好,而只回答了一半就和介绍了下国产的血球分析仪有那个几个大的厂家,没有和区分那个最好那个第二那个次点,或许一堆鸡蛋里实在挑不出骨头。
具所知呢迈瑞的好点特康实力没有迈瑞好,迈瑞吸引了一批外资在搞研发,桂林的呢好像有个优利特现在出血分析仪,他做的尿分析仪市场的占有还可以,血的反应不是很强烈。
实在是想找个内行的给比较下。
以前用countstar,一块计数板5个孔,后来实验室又换了biorad的TC20,一块板子两个孔,只能计两个数,一盒板子30片500块,价格越来越贵。这样我一个12孔板计个数就要花百来块钱啊。大家都是这么用的?板子是塑料的,有没有什么可以清洗之后重复利用的方法?
首先,电子细胞技术仪是机器计数,全自动计数,快速准确,比人工有效率多了,有条件可以购买一台计数器咯~~bodboge是我们在用的机器
其次,细胞计数板需要人工操作,取样什么的都要注意,不然就会有偏差,而且速度还很慢
所以,总的来说,肯定是机器计数会比较准确

