Recombinant Human His6-S5a/Angiocidin Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
SP-400
| Formulation | Supplied as a solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: S5a/Angiocidin
S5a/Angiocidin, also known as Anti-secretory Factor (ASF), is classified under the gene PSMD4, but is often referred to by a different name depending on the context in which it is described. S5a and ASF have identical 377 amino acid (aa) sequences, while Angiocidin is described as having an additional Gly255Glu256Arg257 sequence in its C-Terminus (1-3). The human protein shares 96% and 99% aa sequence identity with its mouse and rat orthologs, respectively. Structurally, it contains an N-terminal von Willebrand Factor type A domain and two C-terminal Ubiquitin-interacting motifs (UIM). It acts as a Ubiquitin-binding protein where it is most commonly referred to as S5a or in yeast as Rpn10. It is part of the 19S regulatory subunit of the 26S Proteasome where its UIM recognizes poly-ubiquitinated proteins destined for degradation (4). As a part of the proteasome complex, it may also recognize the Ubiquitin-like modifier FAT10 (5). Free cytoplasmic forms also exist where its ubiquitination is catalyzed by a range of Ubiquitin E3 ligases from different classes. Therefore, experimentally S5a/Angiocidin may act as a useful substrate to monitor the activity of (E3) ligases, independent of their specific mechanisms of action (6). In cancer biology, where it is often referred to as Angiocidin, it is shown to slow tumor progression. It is found in the extracellular matrix of certain tumor subtypes, and it may act by suppressing angiogenesis or by directly inhibiting tumor cell growth (7,8). It also is found in several biological fluids where it is known primarily as ASF. It suppresses fluid secretion in response to enterotoxin and may act as an anti-inflammatory factor (9-11).
- Nilsson, L.A. et al. (1992) J. Parasitol. 78:1055.
- Young, P. et al. (1998) J. Biol. Chem. 273:5461.
- Zhou, J. et al. (2004) J. Cell. Biochem. 92:125.
- Walters, K.J. et al. (2002) Biochemistry 41:1767.
- Rani, N. et al. (2011) Nature Commun. 3:749.
- Kim, H.T. & A.L. Goldberg (2012) Methods Mol. Biol. 832:653.
- Sabherwal, Y. et al. (2006) Exp. Cell Res. 312:2443.
- Godek, J. et al. (2011) Exp. Mol. Pathol. 90:244.
- Davidson, T.S. & W.J. Hickey (2004) J. Leukoc. Biol. 76:835.
- Davidson, T.S. & W.J. Hickey (2004) Lab. Invest. 84:307.
- Ulgheri, C. et al. (2010) Nutr. Res. Rev. 23:300.
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不知道有没有这样的仪器？
型 号：IC1000
品 牌：Countstar
单 价：45000.00元/台
最小起订量：1 台
供货总量：5 台
发货期限：自买家付款之日起 7 天内发货
6840临床检验分析仪器
首先，电子细胞技术仪是机器计数，全自动计数，快速准确，比人工有效率多了，有条件可以购买一台计数器咯~~bodboge是我们在用的机器
其次，细胞计数板需要人工操作，取样什么的都要注意，不然就会有偏差，而且速度还很慢
所以，总的来说，肯定是机器计数会比较准确
