AQUApure Di-Ub Chains (K6-linked) Protein, CF Summary
Ub-AQUA analysis:
K6 : 99.19%
K63: 0.40%
K48: 0.22%
K11: 0.14%
All other linkages ≤ 0.03%
Ubiquitin chains vary in length, linkage, and function. K6-linked Di-Ubiquitin Chains (Ub2) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °c="" for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-11B
| Formulation | 1 mg/ml (58 μM) in sterile, deionized water |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Di-Ubiquitin
Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB"s") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF"s and/or other Ubiquitin-sensing elements.
K6-linked Di-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates. The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.
- Kirkpatrick D.S., et al. (2006) Nat Cell Biol. 8(7): 700-10
- Ordureau, A., et al. (2014) Mol. Cell 56(3): 360–375
- Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA 112(21): 6637–6642
- Phu L., et al. (2011) Mol Cell Proteomics 10(5): M110.003756
Citations for AQUApure Di-Ub Chains (K6-linked) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
8Citations: Showing 1 - 8Filter your results:
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- The ubiquitin interacting motifs of USP37 act on the proximal Ub of a di-Ub chain to enhance catalytic efficiencyAuthors: N Manczyk, G Veggiani, J Teyra, AW Strilchuk, SS Sidhu, F SicheriSci Rep, 2019;9(1):4119.Applications: Bioassay
- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- PINK1 autophosphorylation is required for ubiquitin recognitionAuthors: S Rasool, N Soya, L Truong, N Croteau, GL Lukacs, JF TrempeEMBO Rep., 2018;0(0):.Species: Insect - Tribolium castaneumSample Types: Recombinant ProteinApplications: Bioassay
- OTUD4 Is a Phospho-Activated K63 Deubiquitinase that Regulates MyD88-Dependent SignalingAuthors: Y Zhao, MC Mudge, JM Soll, RB Rodrigues, AK Byrum, EA Schwarzkop, TR Bradstreet, SP Gygi, BT Edelson, N MosammaparMol. Cell, 2018;69(3):505-516.e5.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidationAuthors: YJ Wang, Y Bian, J Luo, M Lu, Y Xiong, SY Guo, HY Yin, X Lin, Q Li, CCY Chang, TY Chang, BL Li, BL SongNat. Cell Biol., 2017;19(7):808-819.Species: N/ASample Types: Applications: Control
- Characterization of the deubiquitination activity and substrate specificity of the chicken ubiquitin-specific protease 1/USP associated factor 1 complexAuthors: H Zheng, M Wang, C Zhao, S Wu, P Yu, Y Lü, T Wang, Y AiPLoS ONE, 2017;12(11):e0186535.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- In�Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the ProteasomeAuthors: H Tsuchiya, F Ohtake, N Arai, A Kaiho, S Yasuda, K Tanaka, Y SaekiMol. Cell, 2017;66(4):488-502.e7.Applications: Bioassay
- Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin ChainsAuthors: F He, HP Wollscheid, U Nowicka, M Biancospin, E Valentini, A Ehlinger, F Acconcia, E Magistrati, S Polo, KJ WaltersCell Rep, 2016;14(11):2683-94.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
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不知道有没有这样的仪器？
型 号：IC1000
品 牌：Countstar
单 价：45000.00元/台
最小起订量：1 台
供货总量：5 台
发货期限：自买家付款之日起 7 天内发货
6840临床检验分析仪器
首先，电子细胞技术仪是机器计数，全自动计数，快速准确，比人工有效率多了，有条件可以购买一台计数器咯~~bodboge是我们在用的机器
其次，细胞计数板需要人工操作，取样什么的都要注意，不然就会有偏差，而且速度还很慢
所以，总的来说，肯定是机器计数会比较准确
