Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 22222 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV1 | 22222-AAV1 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV9 | 22222-AAV9 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backboneAAV-FLEX(Search Vector Database)
- Backbone manufacturerScott Sternson
- Backbone sizew/o insert(bp)5000
- Vector typeMammalian Expression, AAV, Cre/Lox ; adeno-associated virus
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)NEB Stable
- Growth instructionsUse recombinase-free E. coli (e.g., Invitrogen's Stbl3), grow at 30C
- Copy numberLow Copy
Gene/Insert
- Gene/Insert nameArch
- Alt nameArch-GFP
- Alt namearchaerhodopsin-3
- Alt nameH. sodomense archaerhodopsin-3
- SpeciesH. sodomense
- Insert Size (bp)1500
- Mutationno
- PromoterCAG
- Tag/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteKpnI(not destroyed)
- 3′ cloning siteBsrGI(not destroyed)
- 5′ sequencing primerCCCATAACTTCGTATAATGTATGC (Common Sequencing Primers)
Resource Information
- Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
- genOway Notice of RIghts
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 15 References
Depositor Comments
PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.
In the map below, the Selected Features list should be labeled as follows (instead of the 3 loxP sites listed from 3629-3692 and 5299-5403): lox2272: 3629-3596 , loxp: 3725-3692 lox2272: 5266-5399 , loxp: 5370-5403
Information for AAV1 (Catalog # 22222-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA.
CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition.These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneGFP (Cre-dependent)
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Using FLEX vectors in vivo: LoxP sites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activated (i.e., "flipped") viral vectors. Addgene has measured this occurs in 0.01-0.03% of viral vectors in our typical production protocol. This can lead to a small number of cells exhibiting Cre-independent transgene expression in vivo. To address this, we recommend titrating to find the optimal AAV dosage required for Cre-dependent transgene expression and function in vivo. This may include reducing the viral vector dosage in order to reduce the likelihood of Cre-independent expression.
Information for AAV9 (Catalog # 22222-AAV9)(Back to top)
Purpose
Ready-to-use AAV9 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA.
CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition.These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneGFP (Cre-dependent)
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Using FLEX vectors in vivo: LoxP sites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activated (i.e., "flipped") viral vectors. Addgene has measured this occurs in 0.01-0.03% of viral vectors in our typical production protocol. This can lead to a small number of cells exhibiting Cre-independent transgene expression in vivo. To address this, we recommend titrating to find the optimal AAV dosage required for Cre-dependent transgene expression and function in vivo. This may include reducing the viral vector dosage in order to reduce the likelihood of Cre-independent expression.
Data submitted about 22222-AAV9 by requesting scientist(s):
- Data 1: Mouse, Brain parenchyma
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简介：天平，亦称天秤，在物理学上，是一种利用作用在物体上的重力以平衡原理测定物体质量或确定作为质量函数的其他量值、参数或特性的仪器。由支点（轴）在梁的中心支着天平梁而形成两个臂，每个臂上挂着一个盘，其中一个盘里放着已知质量的物体，另一个盘里放待测物体，固定在梁上的指针在不摆动且指向正中刻度时的偏转就指示出待测物体的质量。向左转|向右转基本结构：
普通天平主要由立柱、横梁、吊挂系统、底座和制动装置组成。立柱垂直固定在底座上，用以支撑横梁。立柱下部装有分度牌，顶部装有托架，在天平不工作时支托横梁。在横梁中部装有一把中刀。天平工作时，中刀搁置在与升降杆顶端连接的刀承上，作为支点。中刀两边装有两把边刀，分别作为重点和力点，起承受和传递载荷的作用。中刀下横梁底面装有指针，指针上固定有可上下移动以调节横梁重心位置的重心砣，它起调整天平灵敏度的作用。横梁顶部刻有分度标尺，标尺上有一移动游码。横梁两端还装有可调整天平空载平衡位置的平衡螺母。吊挂系统包括小吊环，挂盘架和秤盘。挂盘架吊挂在小吊环吊钩上，两把边刀分别通过小吊环承受秤盘砝码和被称物的重力。底座装有两个调整天平水平的螺旋调整脚，底座上面还安置有水准器以显示天平水平度。调整水平是为避免天平不水平而产生称量误差。制动装置主要由开关旋钮、开关轴和偏心凸轮（或连杆）组成。转动旋钮使凸轮（或偏心连杆）偏转一定角度，即可使立柱中的升降杆上下移动，通过中刀承将横梁托起或落下，以开启或关闭天平。使用注意事项：1、要放置在水平的地方。2、使用前要使天平左右平衡（游码必须归“0”平衡螺母向相反方向调，使用口诀：左高端，向左调）。3、砝码不能用手拿要用镊子夹取.千万不能把砝码弄湿、弄脏（这样会让砝码腐蚀生锈，砝码质量变大，测量结果不准确），游码也要用镊子拨动。4、被测物体的质量不能超过天平量程或低于天平游码最小刻度。5、潮湿的物体和化学药品不能直接放在天平的盘中。6、称量时注意左物右码（游码示值以左边对齐刻度线为准）。7、称量后要把游码归零，砝码用镊子放回砝码盒。
希望和大家分享这一点小小的经验。
请问，临床前研究用的电子天平，需要符合GMP要求吗？需要3Q认证吗？相关数据用于申报临床试验的，谢谢
二个分度值的具体含义：
1.d是天平的最小刻度，称为分度值，也可叫作最小读数精度。按规定标准称其为天平实际标尺分度值，以符号“d”来代表。
2.e是天平检定标尺分度值，即检定时所用的分度值。检定时用的分度值和天平实际标尺分度值d不同，它不和d值一样是固定不变的，而是需要根据人为规定变化的值。可以规定e=5d，也可以规定e=10d，或者e=2d均可成立，只要不违反国家计量检定规程的规定就可以。
电子天平实际标尺分度值d是表示电子天平的最小精度是多少，检定标尺分度值e是电子天平进行检定时考核技术指标用的。准确的说，看电子天平的最佳精准度不能只看d值，还要看e值。特别是精确称量、科学实验及量值传递等部门在选择精密电子天平如0.01mg电子天平时最好要看检定标尺分度值e。
(2) 向盘中加减砝码时要用镊子，不能用手接触砝码，不能把砝码弄湿、弄脏；（否则，砝码会沾上污物或被手上的汗水等锈蚀砝码，造成称量结果不精确）
(3) 潮湿的物体和化学物品不能直接放到天平盘中；（否则，会腐蚀天平托盘，减少天平使用寿命）
另外，还应注意：
(4) 搬动天平时，应轻拿轻放，不可让天平受到强烈振动；（否则，会损伤天平，造成天平的称量的灵敏度和精确度降低或减少天平的使用寿命）
(5) 用毕，应将游码归零，砝码归盒；横梁用固定圈固定，保证支架刀口不受损伤（此条适用于学生天平）；（否则，会损伤天平，造成天平的称量的灵敏度和精确度降低或减少天平的使用寿命。）
亲：希望我的回答能够帮助到你，如果满意，请采纳或点赞支持，给我更多助人的动力！
我公司新购进的一台梅特勒XUS205U的十万分之一天平，他可以进行十万分之一与万分之一的转换，d值0.01mg/0.1mg跟随工程师做了四角偏差，重复性的测试，关于最小称样量工程师拒绝给做。想请教各位有没有知道怎么做的。目前了解的就是用1g的砝码做标准偏差然后反推，但是还不是很清楚怎么做
