ProductDescription
ElectrospunGelatinDiscsarefabricatedusingelectrospinningtechniquestocreateaconstructwithauniquestructuresuitableasabiomimeticthree-dimensional(3D)scaffold.Theproductcontainsoverlaidelectrospunfilamentscreatingaporousnetwork,whichpermitscellsandnutrientstoflowcompletelythroughtheporesandprovidesanincreasedsurfaceareaforcellattachment,growthandmigration.
ElectrospunGelatinDiscsarelightlycross-linkedforincreasedmechanicalstrengthanddurABIlityforshortandlong-termtissuecultureexperiments,yetisstillbiodegradableoverthelonger-term.Theaveragefilamentdiametertypicallyrangesfrom500nmto1.5microns.Theelectrospungelatindiscsareapproximately8mmindiameterand1-2mmthick.Theelectrospundiscsfitintoa48wellcultureplateorlarger.Eachpackagecontainsfivediscs.Thisproductissterilizedandready-to-use.
Table1:
| Parameter,Testing,andMethod | ElectrospunGelatinDiscs#5214 |
| DiscDiameter | 8mm |
| PackageSize | 5Discs/Package |
| DiscThickness | 0.1-0.2mm |
| FiberDiameter | 500nm-1.5um |
| StorageTemperature | RoomTemperature |
| ShelfLife | Minimumof6monthsfromdateofreceipt |
| SterilizationMethod | IrrADIation |
| GelatinSource | PorcineGelatin |
DirectionsforUse
DownloadthefullPDFversionorcontinuereadingbelow:
- PreparationandSeeding:
Note:Cellattachmenttothediscisgenerallythemostcriticalstepintissueculture.Temperature,pH,gasexchangeandcellconcentrationcanaffecttherateandefficiencyofattachment.Optimumseedingratedependsonthetypeofcellbeingcultured.
- AsepticallyremovetheElectrospunGelatinDiscsfromthepackaginginalaminarflowworkstation.
- Carefullyplacethediscsintothewellsofa48welltissuecultureplateorlargerusingasterileinstrument.Becarefulnottodamagetheproductasitisbeingtransferred.Itisrecommendedtousenon-treatedtissuecultureplasticware.Note:Tissue-coatedplasticwaremayneedtobecoatedwithagarosetopreventcellattachmenttotheplasticandpromoteattachmenttothedisc.
- SUSPendcellsatdesiredconcentration(approximately5,000cells/cm2)anddispensesufficientvolumeofcellsolutionontopofthediscplacedinthewell.
Note:Avoiddispensingthecellsolutiontoorapidlyasthismaycausedamagetothesponge.
- Transfertoa37ºCincubatorforabout1–2hourstoallowforinitialcellattachment.
- After1–2hour,removetheplatefromtheincubatorandcheckforcellattachment.Additionaltestingmayberequiredtooptimizethetimeittakesforthecellstoattachtothediscs.Checkthemorphologyofthecells.Celladherenceandspreadingwilldictatethetimeneededforattachment.
- Oncethecellshaveadequatelyattachedtothedisc,increasethefinalvolumeineachwelltofullycoverandprovideadequatemediumfortheculturesystem.
- ChangingtheMedia:
- Changethemedia24to36hoursaftertheinitialseeding.Thefrequencyofchangeswillbedeterminedbycelltype,cellattachmentefficiency,andpH.Morefrequentmediumchangesmayberequiredcomparedto2Dculturesystems.
- HarvestingofCells:
Note:Proteasedigestionisthestandardmethodofreleasingcellsfromthediscs.Thestrengthoftheattachmentofthecellstothediscswillvaryfromcelllinetocellline.Theenzymeconcentrationanddigestiontimewillvarydependingupontheactivityoftheenzymeandtheconfluenceofthecells.Collagenaseand/ortrypsinmaybethepreferredmethod.
- WashingthediscswithEDTA-PBSmayassisttheproteasedigestion.Addsufficientvolumetocoverthedisc.
- AspiratetheEDTA-PBSsolutionfromthewell.
- Addsufficientdissociationsolutiontothewelltofullysubmergethediscs.
- Transfertoa37ºCincubator.Checkforcelldetachmentperiodically.
- Oncethecellshavefullydetached,removethecellsanddispenseinacentrifugetube.
- Centrifugethecellsasrequired.
ProductCertificateofAnalysis
SafetyandDocumentation
SafetyDataSheet
CertificateofOrigin
ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
美国AdvancedBioMatrix（简称ABM） www.advancedbiomatrix.comAdvancedBioMatrix（简称ABM）是美国一家著名的生物公司，获得了AllerganInc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺)，将Allergan的专业和技术用于蛋白生产与检测，致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。AdvancedBioMatrix不断丰富已有产品线，目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。AdvancedBioMatrix是组织培养，细胞分析和细胞增殖三维（3D）应用的生命科学领域的领导者。我们的产品被公认为纯度，功能性和一致性的标准。我们在生产，分离，纯化，冷冻干燥，细胞培养和蛋白质测试，粘附肽，附着因子，底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。我们的专业技术和知识正在被用来确保我们的产品质量最高，批次之间一致且易于为我们的研究客户使用。
美国AdvancedBioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。AdvancedBioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3Dmatrix 产品开发方面有着丰富的经验。AdvancedBioMatrix的研发经验和专业知识确保其产品可达到最佳质量，并保证产品之间一致性，方便研究客户使用。以下为AdvancedBioMatrix3DMatrices 产品竞争优势：1. 提供高纯度和成分确定的胞外基质；2. 超过1000余篇文献引用PureCol产品，品质非常均一；3. 在3D培养基领域可提供最全面的产品线；4. 唯一可提供特异性刚性有机硅基板的公司（CytoSoft）;5. 唯一可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）；6. 如果客户首次接触3D胶原凝胶，AdvancedBioMatrix还是唯一的预制胶原蛋白(PureColEZGel)供应商；
以下产品为AdvancedBioMatrix全球畅销品：1.PureCol 牛源I型胶原蛋白 3mg/ml#5005-100ML2.Nutragen牛源I型胶原蛋白 6mg/ml#5010-50ML3.FibriCol 牛源I型胶原蛋白 10mg/ml#5133-20ML4.VitroCol 人源I型胶原蛋白 #5007-20ML5. 弹性蛋白原 #5052-1MG6.ECMSelectArraykitUltra-36#5170-1EA7.CytoSoft（刚性可变的基底，AdvancedBioMatrix最新添加产品5190-7EA）8. 人III型胶原蛋白 #5021-10MG9. 人IV型胶原蛋白 #5022-5MG10.SilkFibroin溶液 #5154-20ML11.Fibronectin#5080-5MG12.Vitronectin#5051-0.1MG
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
浓度的测定，需测定在230、260和280nm处的消光值，经验数据表明，纯净的核酸溶液A260/A230的消光值比大于或等于2.0；A260/A280大于或等于1.80。A260/A280值过小，说明蛋白未脱净；A260/A230过小，说明有杂质（一般为多酚类或色素）。
这样的提问没有意义
建议自己下去查查资料
仪器性能：拍摄对象：核酸琼脂糖凝胶电泳X光胶片功能：获取并处理图像测定图像光密度操作步骤：1. 开启成像系统，电脑电源2. 打开Quality One，调整光源所示图像3. 观察并调整曝光时间，获得图像并保存4. 调整图像，并对图像进行光密度分析5. 关闭所有电源
一是紫外灯照射出问题了；
也可能是门没关紧，经常接触又断掉，不稳定；
如果成像仪没问题，那很可能就是电脑上操纵拍照的软件在设置上有了问题，应该有配套的说明书的，可以参考；
实在不行，可以报修的，没必要自己找烦恼。
