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Addgene/pENN.AAV.CB7.CI.eGFP.WPRE.rBG/1unit/105542-AAV8
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105542-AAV8
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4000-520-616

Ordering

ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid105542Standard format: Plasmid sent in bacteria as agar stab1$75
Add to Cart
AAV1105542-AAV1Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV2105542-AAV2Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV5105542-AAV5Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV8105542-AAV8Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV9105542-AAV9Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information
$380
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
    (Search Vector Database)
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP
  • PromoterCB7

Cloning Information

  • Cloning methodUnknown

Resource Information

  • Supplemental Documents
    • pENN.AAV.CB7.CI.EGFP.WPRE.RBG (P1963).gb
  • Terms and Licenses
    • UBMTA
    • Ancillary Agreement for Plasmids Containing FP Materials
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
    • 2 References

Depositor Comments

Penn Vector Core number (1963

Information for AAV1 (Catalog # 105542-AAV1)(Back to top)

Purpose

Ready-to-use AAV1 particles produced from pENN.AAV.CB7.CI.eGFP.WPRE.rBG (#105542). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.eGFP.WPRE.rBG plasmid DNA.

CB7-driven eGFP. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV1
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 (Catalog # 105542-AAV2)(Back to top)

Purpose

Ready-to-use AAV2 particles produced from pENN.AAV.CB7.CI.eGFP.WPRE.rBG (#105542). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.eGFP.WPRE.rBG plasmid DNA.

CB7-driven eGFP. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 7×10¹² vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV2
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 (Catalog # 105542-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pENN.AAV.CB7.CI.eGFP.WPRE.rBG (#105542). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.eGFP.WPRE.rBG plasmid DNA.

CB7-driven eGFP. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 7×10¹² vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV5
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 (Catalog # 105542-AAV8)(Back to top)

Purpose

Ready-to-use AAV8 particles produced from pENN.AAV.CB7.CI.eGFP.WPRE.rBG (#105542). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.eGFP.WPRE.rBG plasmid DNA.

CB7-driven eGFP. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV8
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 (Catalog # 105542-AAV9)(Back to top)

Purpose

Ready-to-use AAV9 particles produced from pENN.AAV.CB7.CI.eGFP.WPRE.rBG (#105542). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.eGFP.WPRE.rBG plasmid DNA.

CB7-driven eGFP. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV9
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

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蚂蚁淘(www.ebiomall.cn)是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台, 该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁 淘搜索全球供应信息,找到合适的产品后在蚂蚁淘下单,然后蚂蚁淘的海外买手进行跨境采购、 运输到中国口岸,最后由蚂蚁淘国内团队报关运输给客户...
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精子密度仪是一种是专门用于测量悬浮液中细胞密度在600nm的小型易用工具,适于测量所有类型的细胞增长率,包括大肠杆菌、酵母,设计的还可以和其它分光光度仪的数据进行比较。... 查看更多>
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河南省伟信招标管理咨询有限公司受洛阳市妇女儿童医疗保健中心的委托,对洛阳市妇女儿童医疗保健中心超声骨密度仪和孕期营养门诊诊疗系统设备采购项目进行公开招标,欢迎符合资格条件的投标人积极参加投标。一、项目名称:洛阳市妇女儿童医疗保健中心超声骨密度仪和孕期营养门诊诊疗系统设备采购项目二、招标编号:HNWXLY(2018)122号三、招标项目简要说明: 1、标段划分:本次招标共划分为2个标段 ,其中Ⅰ标 查看更多>
工作原理简述装有粉末或颗粒的刻度量筒固定在机械振动装置上,振动电机带动机械振动装置垂直上下振动,装有粉或颗粒的刻度量筒随机械振动装置而发生有节拍的振动,随着振动次数的增加,刻度量筒里的粉末或颗粒逐渐振实,振动次数达到设定的次数后,机械振动装置停止振动,读出刻度量筒的... 查看更多>
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商品咨询
一般卖实验器材的地方都有吧,只是精度和质量会有区别,要是仪器的话,淄博盛康电气专业生产密度仪,有其他问题您可以电话咨询一下!
专为薄膜开发的大成精密软X射线面密度厚度测量仪可以精准的测量精度、良好的实现陶瓷隔离膜的厚度和面密度监控。
请高手详细介绍一些western与免疫组化,(除了钱和可操作性之外)可不可以说western更准确呢。

各位大侠:

颗粒筛分及检测堆实密度的意义何在?检测堆实密度时的振荡次数一般是多少?是统一规定的还是各国有各自的要求?

请不吝赐教!!!小弟在此先抱拳谢过。。。

L表示亮度(Luminosity),a表示从洋红色至绿色的范围,b表示从黄色至蓝色的范围。 去看看Lab色彩空间就知道是什么回事了。
差距大很正常,灌砂法测压实度受人为操作影响太大,我在学校一般只采用一种压实度测定方法得到的数据,比如核子密度仪法
Xrite爱色丽的eXact密度仪听说是触屏的,比之前的528升级了,不知道好用么?
超声波骨密度仪的适用群体范围更广,可涵盖所有的年龄层,而双能X线骨密度仪则具有一定的局限性。 海力孚超声波骨密度仪为您解答
TB核子密度仪123
hgk7002017-10-24
标准计数作出来的数据不准要自己输入一组数字应该怎么设定
基本上的电器都带有一定的辐射吧,只是多少问题,基本上不存在问题,电器产品基本都是经过国标的(个人观点)
结合磁珠实验资讯123
wanglemimamima2017-10-24
当然需要!没有最大干密度怎么得出压实度呢!
橡胶密度计,选电子直读式的橡胶密度计。

通过测量两次重量,直接数字显示密度值,自动得出密度结果,省去人工计算。

选橡胶密度计,还需要看橡胶密度计的质量,精度,专业性等多方面来考量。

希望你能选到满意的橡胶密度计,如有问题可咨询仪特诺,也可继续追问我。
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