
Format : | Purified |
Amount : | 100 µg |
Isotype : | Mouse IgG1 Kappa |
Purification : | Protein G Chromatography |
Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Gene : | DNMT1 |
Gene ID : | 1786 |
Uniprot ID : | P26358 |
Alternative Name : | DNMT1, AIM, CXXC9, DNMT |
Immunogen Information : | A partial length recombinant DNMT1 protein (amino acids 128-429) was used as the immunogen for this antibody. |
Dnmt1(DNA (cytosine-5-)-methyltransferase 1 ) is one of the most abundant DNA methyltransferase in mammalian tissues, where it associates with the replication machinery and restores symmetrical methylation at hemimethylated CpG sites generated by the semi-conservative DNA replication process. Dnmt1 comprises a regulatory N-terminal region and a C-terminal catalytic domain connected by a linker of seven glycine-lysine repeats. The N-terminal part contains a PCNA binding domain (PBD), a heterochromatin targeting sequence (TS), a CXXC-type zinc finger domain and two Bromo-Adjacent Homology domains (BAH1 and BAH2). The C-terminal domains of mammalian Dnmts contain all ten catalytic motifs identified in bacterial DNA (cytosine-5) methyltransferases. Dnmt1 maintains methylation patterns with high fidelity and is essential for embryonic development and genome integrity. The molecules interacting with Dnmt1, including RNA polymerase II, some RNA-binding proteins, and some specific Dnmt1-inhibitory RNA molecules are involved in chromatin organization, DNA repair, cell cycle regulation, and apoptosis.
Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml,FACS analysis: 0.5 µg/10^6 cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Nucleus |
Post transnational modification: | Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome. |
Tissue Specificity: | Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. |
BioGrid: | 108123.92 interactions. |
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各位大侠:
颗粒筛分及检测堆实密度的意义何在?检测堆实密度时的振荡次数一般是多少?是统一规定的还是各国有各自的要求?
请不吝赐教!!!小弟在此先抱拳谢过。。。
核子密度仪功能:
•可以和你所有的EDG共享数据,并且可以输出Excel数据表格;
•下载工作数据、土模数据;
•从下载的工作数据中创建客户端报告;
•从下载的土模数据中生成报告;
•检测位置的访问地图,使用Google Earth;
•每个检测都会标出时间/日期和GPS;
•针对中国用户提供了中文视频演示光盘,可让您轻松操作使用EDG。
通过测量两次重量,直接数字显示密度值,自动得出密度结果,省去人工计算。
选橡胶密度计,还需要看橡胶密度计的质量,精度,专业性等多方面来考量。
希望你能选到满意的橡胶密度计,如有问题可咨询仪特诺,也可继续追问我。
纯水校正时,请检查测量池中没有任何气泡。
请更改参数,放宽校正判断的基准值。
百度搜索:上海劳瑞仪器设备
还不错,希望你采纳。

