- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody against Thr181 phosphorylated Tau protein
Purification:Protein G affinity purified
Target Protein:Human Thr181 phosphorylated Tau
Immunogen:KLH conjugated with a short peptide [Ala-Pro-Lys-Thr(p)-Pro-Pro-Ser-Ser-Gly-Glu-Cys], corresponding to the amino acid sequence 177-187 on the phosphorylated Tau protein.
Fusion Myeloma:Sp2/0-Ag14
Specificity:This antibody reacts with the unconjugated Thr181 phosphorylated Tau peptide.
Cross-reactivity:Cross-reactivity with un-phosphorylated peptide [Ala-Pro-Lys-Thr-Pro-Pro-Ser-Ser-Gly-Glu-Cys] was observed.
Species Reacitvity:Human, mouse
Host / Isotype: Mouse, IgG2a Kappa
Formulation:Lyophilized from a solution in 0.01M PBS pH7.2
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL. Avoid repeated freeze and thaw cycle.
Storage: Store at -20oC
Research Area:Alzheimer’s disease and other neurodegenerative diseases
Background:
Tau is a highly soluble microtubule associated protein found in high concentration in neuron of central nervous system. The main function of the protein is stabilizing the microtubule in nerve fibre (axon). There are six tau isoforms which are formed by alternate splicing. Hyperphosphorylation of Tau protein results in aggregation and formation of neurofibrillary tangles, which is significantly associated with cognitive impairment in Alzheimer’s disease. Tau Thr181 phosphorylation has been used as a biomarker for the differentiation of Alzheimer’s disease and other primary dementia such as dementia with Lewy bodies..
Application:
1.Indirect ELISA: The antibody reacts with phosphorylated peptide [Ala-Pro-Lys-Thr(p)-Pro-Pro-Ser-Ser-Gly-Glu-Cys] coated ELISA plate in indirect ELISA. The antibody cross- reacts with un-phosphorylated peptide.
2.Western Blot: The antibody detected a 46kD band in mouse brain tissue lysate.
Detect the mouse brain tissue lysates using antibody (Tau-pho181 2A4) at 1:2000 (0.5 μg/mL) dilution.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Thr181 Phosphorylated Tau, 2A4 |
|---|---|
| Application | EIA, WB |
| Size | 0.1 mg |
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超净工作台操作使用程序及规范(1)操作者需带好一次性口罩、帽子及医用乳胶手套。。(2)用双手轻轻抬起IVC笼盒外端，沿笼架搁挡向外移出笼盒，放在超净工作台旁的不锈钢小推车上。(3)用药液喷雾器充分喷洒双手手套外表及IVC笼盒的外面，消毒灭菌并沾粘住表面的尘埃，防止进入超净台时被层流气体吹扬。也可用带手套的双手浸入药液容器中，捞起并拧干药液毛巾，擦干手套外液滴，同时擦一下笼盒的外表。(4)打开超净工作台移门，把笼盒移入超净工作台。(5)适当拉下超净工作台移门。打开IVC笼盒盖底之间的紧固扣并打开笼盖。侧卧放在一边。更多超净工作台知识可进入上海巴玖查询！
1. 超净台：单人单面的，垂直层流。
2. 倒置显微镜：够基本的观察用就行（但效果要好），无需成像之类的。
3. 液氮罐：两个，30-50升左右的。
4. 用于玻璃移液管的电动移液器一支。
前三个东东都国产的就行，电动移液器可以是进口的，大家给推荐一下，哪个厂家的哪个型号的哪个性价比好些～～（money不多哦）
:):):)
超净工作台只能保护在工作台内操作的试剂等不受污染，并不保护工作人员，而生物安全柜是负压系统，能有效保护工作人员。
而且，紫外在培养基中造成的富氧离子会杀伤细胞。
望采纳^O^
怀着欣喜激动的心情做实验，可是。。。。。可是。。。。。超净台紫外灯忘记关了。。。。。痛心疾首，感觉不会再爱了，我连冻存都没冻过。。。。。
请各位大神指点焦虑的菜鸟，我的细胞系被照了四十多分钟，会有多大的影响，能不能继续用？对人的皮肤会不会有影响，现在过去了几个小时皮肤还挺正常的。
感谢！
