Recombinant Human Tetra-Ub/Ub4 WT Chains (K63/K48/K63), CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UCM-210
| Formulation | Lyophilized from a solution in deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Tetra-Ubiquitin
With a predicted molecular weight of 34 kDa, Tetra-Ubiquitin chains are composed of four Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8). Tetra-Ub can be used to investigate mechanisms of binding and recognition by deubiquitinating enzymes, E3 ligases or other proteins that contain Ubiquitin-associated domains (UBAs) or Ubiquitin-interacting motifs (UIMs). Tetra-Ub is the minimal unit necessary for recognition by the 26S Proteasome and contains structural characteristic (such as repeating hydrophobic patches) not present in di-Ub. This product is made with wild-type human recombinant Ubiquitin and linkage-specific enzymes, which results in one K48-linkage between two K63-linkages.
- Scheffner, M. et al. (1995) Nature 373:81.
- Sharp, P.M. & W.-H. Li (1987) Trends Ecol. Evol. 2:328.
- Behrends, C. & J.W. Harper (2011) Nat. Struct. Mol. Biol. 18:520.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Henry, A.G. et al. (2012) Dev. Cell 23:519.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Zhang, J. et al. (2012) J. Biol. Chem. 287:28646.
Citation for Recombinant Human Tetra-Ub/Ub4 WT Chains (K63/K48/K63), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Vps13D Encodes a Ubiquitin-Binding Protein that Is Required for the Regulation of Mitochondrial Size and ClearanceAuthors: AL Anding, C Wang, TK Chang, DA Sliter, CM Powers, K Hofmann, RJ Youle, EH BaehreckeCurr. Biol., 2018;28(2):287-295.e6.Applications: Bioassay
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超净工作台操作使用程序及规范(1)操作者需带好一次性口罩、帽子及医用乳胶手套。。(2)用双手轻轻抬起IVC笼盒外端，沿笼架搁挡向外移出笼盒，放在超净工作台旁的不锈钢小推车上。(3)用药液喷雾器充分喷洒双手手套外表及IVC笼盒的外面，消毒灭菌并沾粘住表面的尘埃，防止进入超净台时被层流气体吹扬。也可用带手套的双手浸入药液容器中，捞起并拧干药液毛巾，擦干手套外液滴，同时擦一下笼盒的外表。(4)打开超净工作台移门，把笼盒移入超净工作台。(5)适当拉下超净工作台移门。打开IVC笼盒盖底之间的紧固扣并打开笼盖。侧卧放在一边。更多超净工作台知识可进入上海巴玖查询！
1. 超净台：单人单面的，垂直层流。
2. 倒置显微镜：够基本的观察用就行（但效果要好），无需成像之类的。
3. 液氮罐：两个，30-50升左右的。
4. 用于玻璃移液管的电动移液器一支。
前三个东东都国产的就行，电动移液器可以是进口的，大家给推荐一下，哪个厂家的哪个型号的哪个性价比好些～～（money不多哦）
:):):)
超净工作台只能保护在工作台内操作的试剂等不受污染，并不保护工作人员，而生物安全柜是负压系统，能有效保护工作人员。
而且，紫外在培养基中造成的富氧离子会杀伤细胞。
望采纳^O^
怀着欣喜激动的心情做实验，可是。。。。。可是。。。。。超净台紫外灯忘记关了。。。。。痛心疾首，感觉不会再爱了，我连冻存都没冻过。。。。。
请各位大神指点焦虑的菜鸟，我的细胞系被照了四十多分钟，会有多大的影响，能不能继续用？对人的皮肤会不会有影响，现在过去了几个小时皮肤还挺正常的。
感谢！
