- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Key Benefits
- Homogenous assay for active caspase 3/7.
- Breakthrough in cell lysis buffer and preservation of caspase activity.
- Results in a no-wash, one-step assay.
- No need to wash out media from cell samples, just add the reagent directly to your experimental samples.
- Easy to Use: No need to make cell lysates or run Western blots.
- Works with suspension and adherent cells.
Additional information
| Kit Size | 100, 500, 1000 |
|---|
Cell Technology’s APO 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase3/7, form the quenching DEVD sequence, the free dye excites at 488nm and emits at 515-530 nm. As a result of a novel and proprietary Lysis Buffer System, the APO 3/7 HTS Assay is a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.
Figure. In this figure, Jurkat cells were stimulated with various concentrations of staurosporine for 3 hours, after which caspase 3/7 activity was analyzed using the APO 3/7 HTS kit.
| Document Title |
| Apo3HTSprotocol |
| APO 3 HTS Datasheet |
| msds.APO3HTS |
| Reference |
| Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial Killers: ordering caspase activation events in apoptosis. Cell Death and Differ. 6:1067-1074. |
| Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C. R. Ferenz, S. Franklin, T. Ghayur, M. C. Hackett and L. D.Hammill. 1994. Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting Enzyme: A (p20/p10)2 Homodimer. Cell 78:343-352. |
| Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A.Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D. J.Livingston. 1994. Structure and mechanism of interleukin-1 beta converting enzyme. Nature 370: 270-275. |
| Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle,E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N.A. Thornberry and J.W. Becker. 1996. The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7): 619-625. |
| Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066. |
| Alnemri, E.S. et al (1996) Cell 87:171 |
| Trends Biochem Sci 22,388 (1997) |
| Part# | Reagent | Temperature |
| Part# 4004 | Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 1 Vial | -20C |
| Part# 3005 | Cell Lysis Buffer, 1 Bottle | 2-8C |
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一、首先你要明白肽是什么...........................肽是氨基酸通过酰胺键结合而成的东西.........
二、你要明白氨基酸是什么..........................氨基酸是构成蛋白质的基本单位,多种氨基酸结合为长肽链,几条长肽链再盘旋就形成了蛋白质......................
三、关于小分子肽、短肽、多肽、寡肽.......其实都是肽.......区别只是由多少个氨基酸构成而已............
所以,你的问题可以很粗暴地理解为“蛋白质对人体有没有副作用”.......
如果你营养足够的情况下,再补充这个,会导致营养过剩,从而加重身体代谢的负荷............类似就是这样子的了...........小分子肽,一般现在用于化妆品上比较多(一ye子.植物肽面膜就是这个).......小分子肽(可以简单理解为纳米胶原蛋白),这比蛋白质(也可以粗暴理解为胶原蛋白)更加容易吸收......而用在食品上,要视乎是何种小分子肽了.....大豆肽、花生肽、大米肽....不同的肽有不同的功效.......主要可以改善风味、改善吸收、增强胃肠道功能等等.......
就是蛋白质分子的小片断
是氨基酸形成的
Musmusculus2dayspregnantadultfemaleovaryCDNA,RIKENfull-lengthenrichedlibrary,clone:E330021J16product:inferred:SRY-boxcontaininggene4/sox-4,fullinsertsequence。
product:inferred:SRY-boxcontaininggene4/sox-4,fullinsertsequence是什么意思,这个基因推测产物是sox-4?fullinsertsequence是什么意思?
为了使供输血用的血液能保存一定时间,加入适当的抗凝剂,并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低,尚加入葡萄糖。ACD液(含柠檬酸三钠、柠檬酸、葡萄糖)一般用于血库。添加ACD的血液放在4—6℃条件下保存,可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
转运体是介导分子或离子转运跨过生物膜的物质。通常是蛋白质或酶。说得简单一点就是“运输工具”
受体是能与细胞外专一信号分子(配体)结合引起细胞反应的蛋白质。受体与配体结合即发生分子构象变化,从而引起细胞反应,如介导细胞间信号转导、细胞间黏合、细胞胞吞等细胞过程。它起的是“传达员” 的作用
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