StressMarq/Anti-beta 4 Spectrin Antibody [S393-2]/SMC-484D-A594/100-µg_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城

商品信息

联系客服

郑重提醒：

无质量问题不接受退换货，下单前请仔细核对信息。

下单后请及时联系客服核对商品价格，订单生效后再付款。

StressMarq/Anti-beta 4 Spectrin Antibody [S393-2]/SMC-484D-A594/100-µg

品牌 /

StressMarq

货号 /

SMC-484D-A594

官网地址

美元价：

登录查看

(友情提示：该价格仅为参考，欢迎联系客服询价！)

数量：

免费咨询热线

4000-520-616

商品介绍售后保障相关文章商品咨询

联系客服

Overview:

Product Name beta 4 Spectrin Antibody

Description

Mouse Anti-Human beta 4 Spectrin Monoclonal IgG1

Species Reactivity Human, Mouse, Rat

Applications WB, IHC, ICC/IF

Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.

Host Species Mouse

Immunogen Species Human

Immunogen Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin

Concentration 1 mg/ml

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex
Small phycobiliprotein
Isolated from red algae
Large stokes shift (195 nm)
Molecular Weight: 35 kDa

PerCP Datasheet

PerCP Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield
High rate of photobleaching
Good solubility in water
Broad emission spectrum
pH dependent spectra
Molecular formula: C₂₀H₁₂O₅
Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

ATTO 700

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 575 g/mol

ATTO 700 Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

ATTO 680

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 631 g/mol

ATTO 680 Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 633

Overview:

High fluorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in polar solvents
Stable at pH 4 – 11
Cationic dye, perchlorate salt
Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 594

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 1137 g/mol

ATTO 594 Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 565

Overview:

High fluorescence yield
High thermal and photostability
Good solubility in polar solvents
Excellent solubility in water
Very little aggregation
Rhodamine dye derivative
Molar Mass: 611 g/mol

ATTO 565 Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

APC (Allophycocyanin)

Overview:

High quantum yield
Large phycobiliprotein
6 chromophores per molecule
Isolated from red algae
Molecular Weight: 105 kDa

APC Datasheet

APC Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
Molecular weight: 53 kDa
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target
Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors
Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
High turnover rate enables rapid generation of a strong signal
44 kDa glycoprotein
Extinction coefficient: 100 (403 nm)
Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
Molecular weight: 140 kDa
Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum
High quantum yield
Photostable
Member of the phycobiliprotein family
Isolated from red algae
Excellent solubility in water
Molecular Weight: 250 kDa

R-PE Datasheet

R-PE Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

Properties

Storage Buffer	PBS pH7.4, 50% glycerol, 0.1% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Protein G Purified
Clonality	Monoclonal
Clone Number	S393-2
Isotype	IgG1
Specificity	Detects ~>200kDa. Does not cross react with other Beta-spectrins
Cite This Product	StressMarq Biosciences Cat# SMC-484, RRID: AB_2702423
Certificate of Analysis	A 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	BetaIV-spectrin sigma1 Antibody, Non-erythrocytic spectrin beta chain 4 Antibody, Non-erythroidspectrin beta chain 3 Antibody, SPTBN4 Antibody, SPTBN3 Antibody, Spnb4 Antibody, KIAA1642 Antibody, EMBL BAB83243.1 Antibody, Ensembl ENSRNOP00000028393 Antibody
Research Areas	Cell Signaling, Neuroscience, Scaffold Proteins, Scaffolds
Cellular Localization	Cytoplasm, Cell Cortex, Cytoskeleton
Accession Number	NP_066022.2
Gene ID	57731
Swiss Prot	Q9H254
Scientific Background	Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart (1). Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier (2).
References	1. Genes and mapped phenotypes. (n.d.). Retrieved March 12, 2015, from http://www.ncbi.nlm.nih.gov/gene/57731 2. Yang Y., Lacas-Gervais S., Morest D.K., Solimena M., and Rasband M.N. (2004) J Neurosci. 24(33): 7230-7240.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2 (SMC-484). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody (SMC-484) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) beta 4 Spectrin Antibody (D) Composite.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2 (SMC-484). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody (SMC-484) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) beta 4 Spectrin Antibody (D) Composite.

<p>Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2 (SMC-484). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg protein. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (SMC-484) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.</p>

Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2 (SMC-484). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg protein. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (SMC-484) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.

Product Citations (0)

Currently there are no citations for this product.

ATTO 594

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 1137 g/mol

ATTO 594 Datasheet

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

蚂蚁淘电商平台
ebiomall.com

公司简介

蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...

蚂蚁淘承诺

正品保证： 全球直采在线追溯蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权；同时客户可以通过订单详情查看到货物从厂方至客户的所有流程，确保货物的来源；正规报关，提供13%增值税发票。

及时交付： 限时必达畅选无忧蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节；同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上，部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。

轻松采购： 在线下单简单省事蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价；报价单与合同可以直接在线生成或打印；就像在京东购物一样，您的鼠标点击几次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。

售后申请： 耐心讲解优质服务蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时，您可通过我的订单提交您的“申请售后”，蚂蚁淘产品顾问会第一时间为您处理，在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。

重庆市卫生健康委员会

2018-07-16

amresco SM缓冲液【1218】，amresco现货。Amresco公司来自美国，成立于 1976 年，为高质量生化试剂 / 试剂盒的生产商及供应商，产品服务于生物科研领域。用于体外诊断及医药中间体的美国 FDA 注册。amresco SM缓冲液【1218】021-61806666 33779006品牌：AMRESCO数量：大量保存条件：4℃供应商：AMRESCO保质期：1年amresco SM缓冲液【1218】CodeItem 查看更多>

德国Merck Dtube透析管说明书

2021-09-03

上海研生实业有限公司所提供的Citrate 柠檬酸盐缓冲液（0.01M pH6.0）质量可靠、规格齐全,上海研生实业有限公司不仅具有精湛的技术水平,更有良好的售后服务和优质的解决方案,欢迎您来电咨询此产品具体参数及价格等详细信息！查看更多>

miRNA慢病毒构建与包装服务

2018-07-15

本页关键字：amresco总蛋白细胞裂解缓冲液 amresco报价 amresco总蛋白细胞裂解缓冲液，amresco报价。提供amresco总蛋白细胞裂解缓冲液报价、咨询及技术服务，欢迎来电订购amresco总蛋白细胞裂解缓冲液。中国试剂网经营实验试剂、实验耗材、实验仪器及实验技术服务。公司简介 Amresco 是全球著名的生化试剂生产商，其产品种类齐全，质量稳定，性价比好。深受广大用户的喜爱。Amresco公司来自美国，成立于 19 查看更多>

TAE (50X)(Tris乙酸盐EDTA缓冲液)(ST716)

2021-09-09

上海信帆生物科技有限公司在发布的TAE缓冲液, 50X供应信息，浏览与TAE缓冲液, 50X相关的产品或在搜索更多与TAE缓冲液, 50X相关的内容。查看更多>

4%多聚甲醛溶液的配制方法

2021-07-27

北京绿百草科技发展有限公司在发布的4% 多聚甲醛磷酸盐缓冲液4%-Paraformaldehyde Phosphate Buffer Solution供应信息，浏览与4% 多聚甲醛磷酸盐缓冲液4%-Paraformaldehyde Phosphate Buffer Solution相关的产品或在搜索更多与4% 多聚甲醛磷酸盐缓冲液4%-Paraformaldehyde Phosphate Buffer Solution相关的内容。查看更多>

沸石分子筛_供应美国ZEOCHEM分子筛批发沸石分子筛 ...

2021-08-17

Enzymatics国内代理面议上海市上海起发实验试剂有限公司 2016-11-24 在线询价>猜你喜欢硝唑尼特杂质提供图谱深圳远扬化学技术有限公司奈必洛尔杂质对照品标... 查看更多>

吉林省德商药业股份有限公司所属域名dspharm.com.cn备案详细信息

2018-07-16

amresco蛋白浓缩胶缓冲液【1218】，amresco现货。Amresco公司来自美国，成立于 1976 年，为高质量生化试剂 / 试剂盒的生产商及供应商，产品服务于生物科研领域。用于体外诊断及医药中间体的美国 FDA 注册。amresco蛋白浓缩胶缓冲液【1218】021-61806666 33779006品牌：AMRESCO数量：大量保存条件：4℃供应商：AMRESCO保质期：1年amresco蛋白浓缩胶缓冲液【1218】Cod 查看更多>

南方科技大学本科生招生系统

2021-07-23

磷酸盐缓冲液（Phosphate Buffered Saline，简称PBS）的是常用的用于生物学研究的一个缓冲溶液。... 查看更多>

人维生素D(VD)试剂盒(ELISA)

2021-09-21

四川展峥生物科技有限公司在发布的Restore Western Blot 抗体剥离缓冲液供应信息，浏览与Restore Western Blot 抗体剥离缓冲液相关的产品或在搜索更多与Restore Western Blot 抗体剥离缓冲液相关的内容。查看更多>

CAU22114,Biomatik,,Biomatik,+,,Array.Array.Array蚂蚁淘厂家直采.

2021-09-14

北京雷根生物技术有限公司在发布的CGS缓冲液(pH7.0)供应信息，浏览与CGS缓冲液(pH7.0)相关的产品或在搜索更多与CGS缓冲液(pH7.0)相关的内容。查看更多>

Yellow page list of zip 15049, harwick city, page 1 ...

2021-08-25

青岛捷世康生物科技有限公司在发布的Tris-HCl缓冲液(1mol/L,pH9.0)供应信息，浏览与Tris-HCl缓冲液(1mol/L,pH9.0)相关的产品或在搜索更多与Tris-HCl缓冲液(1mol/L,pH9.0)相关的内容。查看更多>

ssc(柠檬酸钠缓冲液)

2018-07-16

amresco SSC缓冲液【1218】，amresco现货。Amresco公司来自美国，成立于 1976 年，为高质量生化试剂 / 试剂盒的生产商及供应商，产品服务于生物科研领域。用于体外诊断及医药中间体的美国 FDA 注册。amresco SSC缓冲液【1218】021-61806666 33779006品牌：AMRESCO数量：大量保存条件：4℃供应商：AMRESCO保质期：1年amresco SSC缓冲液【1218】CodeIt 查看更多>

常见问题

蚂蚁淘所售产品均为正品吗？

蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑； Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。

下单后可以修改订单吗？

未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。

商品几天可以发货？

现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。

订单如何取消？

如订单处于未确定状态，进入“我的订单"页面，找到要取消的订单，点击“取消订单”按钮。

可以开发票吗？

本网站所售商品都是正规清关，均开具13%正规发票，发票金额含配送费金额，另有说明的除外。

如何联系商家？

蚂蚁淘任何页面都有在线咨询功能，点击“联系客服”、“咨询”或“在线咨询”按钮，均可咨询蚂蚁淘在线客服人员，或拨打4000-520-616，除此之外客户可在联系我们页面找到更多的联系方式。

收到的商品少了/发错了怎么办？

同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

退换货/维修需要多长时间？

一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

商品咨询

【求助】关于流动相添加剂分析技术论坛123

快乐药师2021-07-22

我们常用的色谱柱的PH耐受大概是PH（2-9），最近摸条件，但是pH计坏了，想调一下流动相的PH，苦于PH试纸的不精确性，特此求助：

常用流动相加酸碱后PH的总结，希望大家能够提供一点自己测过的结果，谢谢先

短期内输库存血,病人容易发生的酸碱平衡紊乱是() 123

fdsez2015-08-06

相关疾病：高钾血症碱中毒代谢性酸中毒输入库存血，钾离子大量释放入血，会导致高钾血症，同时容易伴发代谢性碱中毒，查了相关资料解释说：大量输...

磷酸钠缓冲液的配制方法 123

高展远瞩2014-10-12

看了GE公司的NiSephrose6FastFlow说明书，说bindingbuffer建议20mMsodiumphosphate,0.5MNaCl,pH7.4,elutingbuffer中是在bindingbuffer中加了高浓度的咪唑，我想问一下，这个怎么配制啊？那个sodiumphosphate是什么？磷酸钠溶液？貌似不具有缓冲性，磷酸钠缓冲液？有可能，但是这个咋配啊？有我能想到的三个配制方法，想求助一下;
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液，再调pH到7.4；（我们试着用这个做了下，发现挂不上柱）
2.配置磷酸钠盐缓冲液：按NaH2PO4：Na2HPO4以19：81的摩尔比配制成pH7.4的缓冲液？（附一张百度出来的配方
）

3.如果是磷酸钠盐缓冲液，可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗？
再者，2和3这两个方法配制的磷酸钠盐缓冲液有什么区别？最终效果是一样的吗？如果不一样，有什么理论的知识支撑呢？个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外，我还想问一下，pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗？是怎么影响的？谢谢大家了！

酸碱加入到缓冲液做的excel的散点图怎么做123

怪小米米2017-10-03

首先你需要有源数据，
有了源数据之后把源数据按照大小排列，
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图

我是菜鸟,请教各位:强酸碱性样品可以用普通C18柱吗?谢谢了~ ...123

yandongliu2021-07-24

我的实验是考察药物不同PH值（水溶液，HCL和NaOH调PH值）下的稳定性情况等，PH从2-13。请问各位大侠：这种强酸性或强碱性水溶液样品可以直接进普通C18柱进行分析吗？
因为是考察不同PH对药物的影响，样品又不好改变其PH值，这种情况怎么办？希望有经验的高手指教。

我的流动相是甲醇-水（90：10）

谢谢赐教！

请进子版按格式发贴，自行修改，谢谢。

各类真空泵原理图解_二哈的世界你永远不懂!_真空泵原理123

2017-10-03

缓冲物质更重要。
由弱酸及其盐、弱碱及其盐组成的混合溶液，能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响，从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。

【求助】蛋白质酸碱性对离子交换色谱填料的选择蛋白质和糖学...123

ldx74226632009-08-05

求助：我目前需用离子交换法进行蛋白质的分离和纯化，但是我不知道要纯化的蛋白质的等电点，怎么样选择柱料及洗脱缓冲液呢？如果必须知道，有没有什么简便的方法测定蛋白质的等电点呢？谢谢

凝胶电泳缓冲液的配制123

haina1682021-07-23

我按下面方法配制电泳缓冲液：
Tris-base15.1g
甘氨酸94g
SDS5g
H2O1000ml
听人说就这样配制，PH值就会在8.3左右，都不要怎么调PH的，但不知我这样配制后PH值在6.4左右？迷惑，配制了几次还是这样。本人初次做sds-page，请前辈们指教。
另外Tris是以前师兄留下的，几年了已经，SDS也是以前的，但是没有开封，密封的很好。甘氨酸是新买进口封装的。会不会试剂过期的原因？

培养基高压蒸气灭菌后pH值的变化及其对细菌生长的影响123

mars7472005-08-25

:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?

一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?

如何选择缓冲溶液?_123

sweetwater2021-07-21

做试验的时候用到很多种缓冲液，Tris,triethanolamine,HEPES,还有磷酸缓冲液，不知道这些缓冲液之间有没有大的区别，选择缓冲液的依据是什么？

如果只是为了缓冲酸碱度，是不是可以用一种缓冲液来代替所有其他的？这样做试验就方便多了。谢谢指教

一种缓冲溶液是一个共轭酸碱的混合物,那么为什么邻苯二甲酸氢钾、四硼 ...123

TWDAFRA01242017-10-03

缓冲溶液是含共轭酸碱对的，其中共轭酸与共轭碱都必须是弱酸或弱碱，这样才能组成缓冲液。因为邻苯二甲酸是弱酸，氨是弱碱，并且它们的同浓度的电离度相似，所以组成的缓冲溶液缓冲能力强。

药典中,纯化水酸碱度能用PH计测量吗?《中国药典》2020版讨论区...123

rainbow09282021-07-25

药典上说取本品10ml，加甲基红指示液（变色范围ph4.2-6.3，红~黄）2滴不得显红色；加溴麝香草酚蓝（变色范围ph6.0-7.6，黄~蓝）5滴不得显蓝色。
是否可以理解为纯化水得PH范围为6.3-7.6？能否直接用pH计测量？谢谢!