A 1:20000 dilution of SMC-212 was sufficient for detection of LC3C in lysate from cells expressing recombinant GST-tagged LC3C by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Biological Description
Alternative Names
MAP1LC3C Antibody, ATG8J Antibody, Autophagy-related protein LC3 C Antibody, Autophagy-related ubiquitin-like modifier LC3 C Antibody, LC3 like protein 2 Antibody, MAP1 light chain 3 like protein 2 Antibody, MAP1 light chain 3 like protein 3 Antibody, MAP1 light chain 3-like protein 3 Antibody, MAP1A/MAP1B LC3 C Antibody, MAP1A/MAP1B light chain 3 C Antibody, MAP1LC3C Antibody, Microtubule-associated protein 1 light chain 3 gamma Antibody, Microtubule-associated proteins 1A/1B light chain 3C Antibody, MLP3C_HUMAN Antibody
Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3C is one of three isoforms which exhibits the lowest expression relative to the other two isoforms. Its highest expression is seen in placenta, lung and ovary.
References
1. Tanida, I., Ueno, T., & Kominami, E. (2008). LC3 and Autophagy. Methods Mol Biol. 445: 77-88.
2. He, H., Dang, Y., Dai, F., Guo, Z., Wu, J., She, X., et al. (2003). J Biol Chem. 278(31): 29278-87.
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Biotin
Properties:
Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C10H16N2O3S
Applications: Western blot, immunohistochemistry, and ELISA
【1】酸碱缓冲溶液：其中的一般酸碱缓冲溶液是指控制溶液酸碱度的溶液.他的作用是在此溶液中,加入少量的强酸或强碱,或者将其稍加稀释时,溶液的ph值基本上保持不变.【2】可以维持溶液酸碱度稳定的原因：主要原因是因为构成一般酸碱缓冲溶液的组分是“一元共轭酸碱”.例如“醋酸-醋酸钠”酸碱缓冲溶液.由PH的计算式：pH = p Ka +log[C酸/C碱] ,可见C酸/C碱的微小改变,其PH值,基本不变.
![StressMarq/Anti-LC3C Antibody [VB2]/SMC-212A-BI/50-µg](images/no_picture.gif)
