Overview:
| Product Name | RhoA Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | Rabbit Anti-Human RhoA Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Applications | WB, IHC | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Antibody Dilution | WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen | A synthesized peptide derived from human RhoA | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet Biotin | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| R-PE (R-Phycoerythrin) | ||
Overview:
R-PE Datasheet | ![]() | Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
Properties
| Storage Buffer | PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
| Storage Temperature | -20ºC |
| Shipping Temperature | Blue Ice or 4ºC |
| Purification | Affinity Purified |
| Clonality | Polyclonal |
| Isotype | IgG |
| Specificity | Detects ~22kDa, endogenous levels of total RhoA |
| Cite This Product | StressMarq Biosciences Cat# SPC-1332, RRID: AB_2713763 |
| Certificate of Analysis | A 1:1000 dilution of SPC-1332 was sufficient for detection of RhoA in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Biological Description
| Alternative Names | Aplysia ras related homolog 12 Antibody, ARH12 Antibody, ARHA Antibody, H12 Antibody, Ras homolog family member A Antibody, Rho A Antibody, RHO H12 Antibody, RHOA Antibody, Small GTP binding protein Rho A Antibody, Transforming protein Rho A Antibody |
| Research Areas | Cancer, Cell Signaling |
| Cellular Localization | Cytoplasm, Cell membrane, Cytoskeleton |
| Accession Number | NP_001655.1 |
| Gene ID | 387 |
| Swiss Prot | P61586 |
| Scientific Background | RhoA is a small GTPase protein of the Rho family. The Rho family acts as molecular swithces and regulators of processes such as cell migration, proliferation and differentiation. RhoA specifically regulates actomyosin contractility, cytokinesis, focal adhesion assembly and cell polarity. |
| References |
1. Bi D., et al. (2005) Circ. Res. 96: 890-897. 2. Kimura K., et al. (2000) J. Biol. Chem. 275: 17233-17236. 3. Barry S.T. and Critchley D.R. (1994) J. Cell Sci. 107 ( Pt 7): 2033-2045. 4. Van Keymeulen A., et al. (2006) J. Cell Biol. 174: 437-445. |
Product Images
Western blot analysis of Human HeLa cell lysates showing detection of ~22kDa RhoA protein using Rabbit Anti-RhoA Polyclonal Antibody (SPC-1332). Lane 1: Human HeLa treated with the immunizing peptide. Lane 2: Human HeLa. Primary Antibody: Rabbit Anti-RhoA Polyclonal Antibody (SPC-1332) at 1:1000. Predicted/Observed Size: ~22kDa.
Product Citations (0)
Currently there are no citations for this product.
| ATTO 488 | ||
Overview:
ATTO 488 Datasheet | ![]() | Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
常用流动相加酸碱后PH的总结,希望大家能够提供一点自己测过的结果,谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液,再调pH到7.4;(我们试着用这个做了下,发现挂不上柱)
2.配置磷酸钠盐缓冲液:按NaH2PO4:Na2HPO4以19:81的摩尔比配制成pH7.4的缓冲液?(附一张百度出来的配方
)
3.如果是磷酸钠盐缓冲液,可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗?
再者,2和3这两个方法配制的磷酸钠盐缓冲液有什么区别?最终效果是一样的吗?如果不一样,有什么理论的知识支撑呢?个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外,我还想问一下,pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗?是怎么影响的?谢谢大家了!
有了源数据之后把源数据按照大小排列,
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响,样品又不好改变其PH值,这种情况怎么办?希望有经验的高手指教。
我的流动相是甲醇-水(90:10)
谢谢赐教!
请进子版按格式发贴,自行修改,谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液,能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响,从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6?能否直接用pH计测量?谢谢!















